Relation between Resistance to Antipseudomonal β-Lactams and ampC and mexC Genes of Pseudomonas aeruginosa
Background: In order to select a better antibiotic choice for treatment of Pseudomonas aeruginosa infections, this study was conducted to determine the frequency of resistance to some antipseudomonal β-lactams in P. aeruginosa isolates from patients in Tehran, Iran. In addition, the relation between...
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| Format: | Article |
| Language: | English |
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Iranian Society of Pathology
2016-01-01
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| Series: | Iranian Journal of Pathology |
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| Online Access: | https://ijp.iranpath.org/article_14583_df11f76839f91abfda21e73161324d0a.pdf |
| _version_ | 1819096906065772544 |
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| author | Fatemeh Rezaei Horieh Saderi Shahram Boroumandi Soghrat Faghihzadeh |
| author_facet | Fatemeh Rezaei Horieh Saderi Shahram Boroumandi Soghrat Faghihzadeh |
| author_sort | Fatemeh Rezaei |
| collection | DOAJ |
| description | Background: In order to select a better antibiotic choice for treatment of Pseudomonas aeruginosa infections, this study was conducted to determine the frequency of resistance to some antipseudomonal β-lactams in P. aeruginosa isolates from patients in Tehran, Iran. In addition, the relation between presence of genes known to be responsible for resistance to β-lactams (ampC, mexC1,2,and mexC3,4 genes) and resistance phenotype among P. aeroginosa isolates was evaluated. Methods: P. aeruginosa strains were isolated and identified by routine methods and PCR for oprL gene. Disk diffusion method was employed to determine the antimicrobial susceptibility pattern according to CLSI recommendations. PCR was used to detect the resistance genes. Results: Among 100 isolates of P. aeruginosa, 82% had ampC, 86% mexC1,2and 89% mexC3,4 genes and combinations of these genes were seen in most of isolates and only 3% of isolates had none of these genes. Resistance to mezlocillin, cefepime, ceftazidime and piperacillin/ tazobactam was seen in 46%, 41%, 36% and 29% of isolates, respectively. Significant relation (P value ≤0.05 by Chi-square or Fisher Exact test) was observed between the presence of ampC gene and resistance to all the studied β-lactams in this study. No relation was observedfor mexC genes,although many ofisolates containing these two genes were phenotypically resistant. Conclusion: This study had shown for the first time, the presence of ampC and mexC genes in significant percent of clinical isolates of P. aeruginosa in Tehran, Iran, and relation between presence of ampC gene and resistance to β-lactams. |
| first_indexed | 2024-12-22T00:06:38Z |
| format | Article |
| id | doaj.art-458c9642105649139ce867682f9e426a |
| institution | Directory Open Access Journal |
| issn | 1735-5303 2345-3656 |
| language | English |
| last_indexed | 2024-12-22T00:06:38Z |
| publishDate | 2016-01-01 |
| publisher | Iranian Society of Pathology |
| record_format | Article |
| series | Iranian Journal of Pathology |
| spelling | doaj.art-458c9642105649139ce867682f9e426a2022-12-21T18:45:32ZengIranian Society of PathologyIranian Journal of Pathology1735-53032345-36562016-01-01111475314583Relation between Resistance to Antipseudomonal β-Lactams and ampC and mexC Genes of Pseudomonas aeruginosaFatemeh Rezaei0Horieh Saderi1Shahram Boroumandi2Soghrat Faghihzadeh3Dept. of Microbiology, School of Medicine, Shahed University, Tehran, IranDept. of Microbiology, School of Medicine, Shahed University, Tehran, IranPars Advanced and Minimally Invasive Research Center, Iran University of Medical Sciences, Tehran, IranDept. of Biostatistics and Epidemiology, Zanjan University of Medical Science, Zanjan, IranBackground: In order to select a better antibiotic choice for treatment of Pseudomonas aeruginosa infections, this study was conducted to determine the frequency of resistance to some antipseudomonal β-lactams in P. aeruginosa isolates from patients in Tehran, Iran. In addition, the relation between presence of genes known to be responsible for resistance to β-lactams (ampC, mexC1,2,and mexC3,4 genes) and resistance phenotype among P. aeroginosa isolates was evaluated. Methods: P. aeruginosa strains were isolated and identified by routine methods and PCR for oprL gene. Disk diffusion method was employed to determine the antimicrobial susceptibility pattern according to CLSI recommendations. PCR was used to detect the resistance genes. Results: Among 100 isolates of P. aeruginosa, 82% had ampC, 86% mexC1,2and 89% mexC3,4 genes and combinations of these genes were seen in most of isolates and only 3% of isolates had none of these genes. Resistance to mezlocillin, cefepime, ceftazidime and piperacillin/ tazobactam was seen in 46%, 41%, 36% and 29% of isolates, respectively. Significant relation (P value ≤0.05 by Chi-square or Fisher Exact test) was observed between the presence of ampC gene and resistance to all the studied β-lactams in this study. No relation was observedfor mexC genes,although many ofisolates containing these two genes were phenotypically resistant. Conclusion: This study had shown for the first time, the presence of ampC and mexC genes in significant percent of clinical isolates of P. aeruginosa in Tehran, Iran, and relation between presence of ampC gene and resistance to β-lactams.https://ijp.iranpath.org/article_14583_df11f76839f91abfda21e73161324d0a.pdfpseudomonas aeruginosaβ-lactamsmexcampc |
| spellingShingle | Fatemeh Rezaei Horieh Saderi Shahram Boroumandi Soghrat Faghihzadeh Relation between Resistance to Antipseudomonal β-Lactams and ampC and mexC Genes of Pseudomonas aeruginosa Iranian Journal of Pathology pseudomonas aeruginosa β-lactams mexc ampc |
| title | Relation between Resistance to Antipseudomonal β-Lactams and ampC and mexC Genes of Pseudomonas aeruginosa |
| title_full | Relation between Resistance to Antipseudomonal β-Lactams and ampC and mexC Genes of Pseudomonas aeruginosa |
| title_fullStr | Relation between Resistance to Antipseudomonal β-Lactams and ampC and mexC Genes of Pseudomonas aeruginosa |
| title_full_unstemmed | Relation between Resistance to Antipseudomonal β-Lactams and ampC and mexC Genes of Pseudomonas aeruginosa |
| title_short | Relation between Resistance to Antipseudomonal β-Lactams and ampC and mexC Genes of Pseudomonas aeruginosa |
| title_sort | relation between resistance to antipseudomonal β lactams and ampc and mexc genes of pseudomonas aeruginosa |
| topic | pseudomonas aeruginosa β-lactams mexc ampc |
| url | https://ijp.iranpath.org/article_14583_df11f76839f91abfda21e73161324d0a.pdf |
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