Association of ovine gammaherpesvirus 2 with an outbreak of acute respiratory disease in dairy cattle
Abstract This study investigated the cause of an outbreak of an acute respiratory disease syndrome followed by episodes of diarrhea in a dairy cattle herd from Southern Brazil. Deep nasal swabs (DNS) from asymptomatic calves, calves with pulmonary discomfort, and diarrheic calves after episodes of r...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-30133-w |
| _version_ | 1797850092463456256 |
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| author | Selwyn Arlington Headley Alais Maria Dall Agnol José Antonio Bessegato Ana Paula Souza Frucchi Érika Fernandes Lopes Maturana Rafael Vince Rodrigues Ana Aparecida Correa Xavier Alice Fernandes Alfieri Amauri Alcindo Alfieri |
| author_facet | Selwyn Arlington Headley Alais Maria Dall Agnol José Antonio Bessegato Ana Paula Souza Frucchi Érika Fernandes Lopes Maturana Rafael Vince Rodrigues Ana Aparecida Correa Xavier Alice Fernandes Alfieri Amauri Alcindo Alfieri |
| author_sort | Selwyn Arlington Headley |
| collection | DOAJ |
| description | Abstract This study investigated the cause of an outbreak of an acute respiratory disease syndrome followed by episodes of diarrhea in a dairy cattle herd from Southern Brazil. Deep nasal swabs (DNS) from asymptomatic calves, calves with pulmonary discomfort, and diarrheic calves after episodes of respiratory distress were used in molecular assays designed to detect the principal pathogens associated with bovine respiratory disease (BRD). Fecal samples were used for the molecular detection of bovine enteric disease agents. Pulmonary tissues from three calves and a cow that died were evaluated by molecular assays to identify 11 agents associated with the development of BRD. The intestinal and pulmonary fragments of one calf and the cow revealed atrophic enteritis and interstitial pneumonia by histopathology, respectively. Immunohistochemistry (IHC) identified intralesional antigens of a malignant catarrhal fever virus, genus Macavirus, within epithelial cells of the lungs and intestines. Molecular assays amplified ovine gammaherpesvirus 2 (OvGHV2) from most of the DNS, and the pulmonary and intestinal fragments from the animals that died, confirming that the Macavirus identified by IHC was OvGHV2. Concomitant pulmonary infections of OvGHV2 with bovine gammaherpesvirus 6 and bovine coronavirus were identified. Additionally, bovine viral diarrhea virus 1b and Aichivirus B were detected in the fecal samples. These findings demonstrated that OvGHV2, a Macavirus, was the disease agent most frequently (81.2%; 13/16) associated with singular pulmonary infections during this outbreak of BRD, suggesting that this virus may be another potential agent of respiratory disease of cattle. |
| first_indexed | 2024-04-09T18:54:47Z |
| format | Article |
| id | doaj.art-458cfd61ec5f4743bb9e96d192014881 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T18:54:47Z |
| publishDate | 2023-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-458cfd61ec5f4743bb9e96d1920148812023-04-09T11:13:50ZengNature PortfolioScientific Reports2045-23222023-04-0113111110.1038/s41598-023-30133-wAssociation of ovine gammaherpesvirus 2 with an outbreak of acute respiratory disease in dairy cattleSelwyn Arlington Headley0Alais Maria Dall Agnol1José Antonio Bessegato2Ana Paula Souza Frucchi3Érika Fernandes Lopes Maturana4Rafael Vince Rodrigues5Ana Aparecida Correa Xavier6Alice Fernandes Alfieri7Amauri Alcindo Alfieri8Laboratory of Animal Pathology, Department of Preventive Veterinary Medicine, Universidade Estadual de LondrinaNational Institute of Science and Technology for Dairy Production Chain (INCT – LEITE), Department of Preventive Veterinary Medicine, Universidade Estadual de LondrinaConsulting Veterinarian, Herd Bovinos - Consultoria PecuáriaLaboratory of Animal Virology, Department of Preventive Veterinary Medicine, Universidade Estadual de LondrinaLaboratory of Animal Pathology, Department of Preventive Veterinary Medicine, Universidade Estadual de LondrinaLaboratory of Animal Pathology, Department of Preventive Veterinary Medicine, Universidade Estadual de LondrinaLaboratory of Animal Pathology, Department of Preventive Veterinary Medicine, Universidade Estadual de LondrinaNational Institute of Science and Technology for Dairy Production Chain (INCT – LEITE), Department of Preventive Veterinary Medicine, Universidade Estadual de LondrinaNational Institute of Science and Technology for Dairy Production Chain (INCT – LEITE), Department of Preventive Veterinary Medicine, Universidade Estadual de LondrinaAbstract This study investigated the cause of an outbreak of an acute respiratory disease syndrome followed by episodes of diarrhea in a dairy cattle herd from Southern Brazil. Deep nasal swabs (DNS) from asymptomatic calves, calves with pulmonary discomfort, and diarrheic calves after episodes of respiratory distress were used in molecular assays designed to detect the principal pathogens associated with bovine respiratory disease (BRD). Fecal samples were used for the molecular detection of bovine enteric disease agents. Pulmonary tissues from three calves and a cow that died were evaluated by molecular assays to identify 11 agents associated with the development of BRD. The intestinal and pulmonary fragments of one calf and the cow revealed atrophic enteritis and interstitial pneumonia by histopathology, respectively. Immunohistochemistry (IHC) identified intralesional antigens of a malignant catarrhal fever virus, genus Macavirus, within epithelial cells of the lungs and intestines. Molecular assays amplified ovine gammaherpesvirus 2 (OvGHV2) from most of the DNS, and the pulmonary and intestinal fragments from the animals that died, confirming that the Macavirus identified by IHC was OvGHV2. Concomitant pulmonary infections of OvGHV2 with bovine gammaherpesvirus 6 and bovine coronavirus were identified. Additionally, bovine viral diarrhea virus 1b and Aichivirus B were detected in the fecal samples. These findings demonstrated that OvGHV2, a Macavirus, was the disease agent most frequently (81.2%; 13/16) associated with singular pulmonary infections during this outbreak of BRD, suggesting that this virus may be another potential agent of respiratory disease of cattle.https://doi.org/10.1038/s41598-023-30133-w |
| spellingShingle | Selwyn Arlington Headley Alais Maria Dall Agnol José Antonio Bessegato Ana Paula Souza Frucchi Érika Fernandes Lopes Maturana Rafael Vince Rodrigues Ana Aparecida Correa Xavier Alice Fernandes Alfieri Amauri Alcindo Alfieri Association of ovine gammaherpesvirus 2 with an outbreak of acute respiratory disease in dairy cattle Scientific Reports |
| title | Association of ovine gammaherpesvirus 2 with an outbreak of acute respiratory disease in dairy cattle |
| title_full | Association of ovine gammaherpesvirus 2 with an outbreak of acute respiratory disease in dairy cattle |
| title_fullStr | Association of ovine gammaherpesvirus 2 with an outbreak of acute respiratory disease in dairy cattle |
| title_full_unstemmed | Association of ovine gammaherpesvirus 2 with an outbreak of acute respiratory disease in dairy cattle |
| title_short | Association of ovine gammaherpesvirus 2 with an outbreak of acute respiratory disease in dairy cattle |
| title_sort | association of ovine gammaherpesvirus 2 with an outbreak of acute respiratory disease in dairy cattle |
| url | https://doi.org/10.1038/s41598-023-30133-w |
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