Pentoxifylline modulation hepatotoxicity and apoptosis induced by nitrosamine in rats
Background: Pentoxifylline (PEN) is a xanthine derivative with different functional characteristics including dilution of blood and increase in tissue oxygenation rate. Nitrosamines (NITs) are well known as strong carcinogenic agents. This study attempts to show the histopathological and biochemical...
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| Format: | Article |
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Wolters Kluwer Medknow Publications
2020-01-01
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| Series: | Biomedical and Biotechnology Research Journal |
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| Online Access: | http://www.bmbtrj.org/article.asp?issn=2588-9834;year=2020;volume=4;issue=3;spage=251;epage=258;aulast=Salahshoor |
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| author | Mohammad Reza Salahshoor Cyrus Jalili Amir Abdolmaleki Shiva Roshankhah |
| author_facet | Mohammad Reza Salahshoor Cyrus Jalili Amir Abdolmaleki Shiva Roshankhah |
| author_sort | Mohammad Reza Salahshoor |
| collection | DOAJ |
| description | Background: Pentoxifylline (PEN) is a xanthine derivative with different functional characteristics including dilution of blood and increase in tissue oxygenation rate. Nitrosamines (NITs) are well known as strong carcinogenic agents. This study attempts to show the histopathological and biochemical effects of PEN against hepatotoxicity induced by NIT in rats. Methods: Sixty-four rats were assigned to eight groups including the groups of sham: NIT (40 mg/kg); PEN (25, 50, and 100 mg/kg); and NIT + PEN. Experimental treatments were applied either intraperitoneally (for NIT) or orally (for PEN) daily for 4 weeks. The relative expression level of p53 and Bax genes and hepatocyte apoptotic index were analyzed. Liver malondialdehyde (MDA), tissue ferric-reducing ability of plasma (FRAP), the diameters of hepatocytes (DH) and central hepatic vein (CHV), and biochemical liver function indicators (LFI) were investigated. Griess technique was hired for the determination of the level of serum nitrite oxide (NO). Results and Conclusions: NIT significantly increased the level of apoptotic gens and index, MDA, NO, diameter of CHV and DH, and LFI and decreased the FRAP level compared to the sham group (P < 0.01). All parameters in PEN and PEN + NIT groups significantly reduced (except FRAP level, which was decreased) in compared to the NIT group (P < 0.01) . By summarizing the results of this research, it is concluded that the PEN administration alleviates the hepatotoxicity due to oxidative stress produced by NIT in rats. |
| first_indexed | 2024-12-11T18:13:14Z |
| format | Article |
| id | doaj.art-458fee9294d44ba5a98f721b06ba94d4 |
| institution | Directory Open Access Journal |
| issn | 2588-9834 2588-9842 |
| language | English |
| last_indexed | 2024-12-11T18:13:14Z |
| publishDate | 2020-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Biomedical and Biotechnology Research Journal |
| spelling | doaj.art-458fee9294d44ba5a98f721b06ba94d42022-12-22T00:55:30ZengWolters Kluwer Medknow PublicationsBiomedical and Biotechnology Research Journal2588-98342588-98422020-01-014325125810.4103/bbrj.bbrj_54_20Pentoxifylline modulation hepatotoxicity and apoptosis induced by nitrosamine in ratsMohammad Reza SalahshoorCyrus JaliliAmir AbdolmalekiShiva RoshankhahBackground: Pentoxifylline (PEN) is a xanthine derivative with different functional characteristics including dilution of blood and increase in tissue oxygenation rate. Nitrosamines (NITs) are well known as strong carcinogenic agents. This study attempts to show the histopathological and biochemical effects of PEN against hepatotoxicity induced by NIT in rats. Methods: Sixty-four rats were assigned to eight groups including the groups of sham: NIT (40 mg/kg); PEN (25, 50, and 100 mg/kg); and NIT + PEN. Experimental treatments were applied either intraperitoneally (for NIT) or orally (for PEN) daily for 4 weeks. The relative expression level of p53 and Bax genes and hepatocyte apoptotic index were analyzed. Liver malondialdehyde (MDA), tissue ferric-reducing ability of plasma (FRAP), the diameters of hepatocytes (DH) and central hepatic vein (CHV), and biochemical liver function indicators (LFI) were investigated. Griess technique was hired for the determination of the level of serum nitrite oxide (NO). Results and Conclusions: NIT significantly increased the level of apoptotic gens and index, MDA, NO, diameter of CHV and DH, and LFI and decreased the FRAP level compared to the sham group (P < 0.01). All parameters in PEN and PEN + NIT groups significantly reduced (except FRAP level, which was decreased) in compared to the NIT group (P < 0.01) . By summarizing the results of this research, it is concluded that the PEN administration alleviates the hepatotoxicity due to oxidative stress produced by NIT in rats.http://www.bmbtrj.org/article.asp?issn=2588-9834;year=2020;volume=4;issue=3;spage=251;epage=258;aulast=Salahshoorapoptosishepatotoxicitynitrosaminepentoxifylline |
| spellingShingle | Mohammad Reza Salahshoor Cyrus Jalili Amir Abdolmaleki Shiva Roshankhah Pentoxifylline modulation hepatotoxicity and apoptosis induced by nitrosamine in rats Biomedical and Biotechnology Research Journal apoptosis hepatotoxicity nitrosamine pentoxifylline |
| title | Pentoxifylline modulation hepatotoxicity and apoptosis induced by nitrosamine in rats |
| title_full | Pentoxifylline modulation hepatotoxicity and apoptosis induced by nitrosamine in rats |
| title_fullStr | Pentoxifylline modulation hepatotoxicity and apoptosis induced by nitrosamine in rats |
| title_full_unstemmed | Pentoxifylline modulation hepatotoxicity and apoptosis induced by nitrosamine in rats |
| title_short | Pentoxifylline modulation hepatotoxicity and apoptosis induced by nitrosamine in rats |
| title_sort | pentoxifylline modulation hepatotoxicity and apoptosis induced by nitrosamine in rats |
| topic | apoptosis hepatotoxicity nitrosamine pentoxifylline |
| url | http://www.bmbtrj.org/article.asp?issn=2588-9834;year=2020;volume=4;issue=3;spage=251;epage=258;aulast=Salahshoor |
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