First Report of Tumorigenic Agrobacterium radiobacter on Raspberry in Serbia
During the spring of 2003, gall symptoms on the roots and crowns of young raspberry plants cv. Vilamette were observed near Valjevo. Phytopathogenic bacteria were isolated from diseased plant samples. Based on the pathogenic, morphological, differential biochemicaland physiological characteristics,...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Institute of Pesticides and Environmental Protection
2007-01-01
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| Series: | Pesticidi i Fitomedicina |
| Subjects: | |
| Online Access: | http://www.pesting.org.rs/download.php/documents/22-2/22-2%20113-119.pdf |
| _version_ | 1818537550064648192 |
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| author | Svetlana Milijašević Veljko Gavrilović Svetlana Živković Nenad Trkulja Joanna Pulawska |
| author_facet | Svetlana Milijašević Veljko Gavrilović Svetlana Živković Nenad Trkulja Joanna Pulawska |
| author_sort | Svetlana Milijašević |
| collection | DOAJ |
| description | During the spring of 2003, gall symptoms on the roots and crowns of young raspberry plants cv. Vilamette were observed near Valjevo. Phytopathogenic bacteria were isolated from diseased plant samples. Based on the pathogenic, morphological, differential biochemicaland physiological characteristics, the isolated strains were identified as tumorigenic Agrobacterium radiobacter (biovar 1 Agrobacterium). In order to confirm the identity of isolated strains by polymerase chain reaction (PCR) primers complementary to tms2 genelocated on the Ti plasmid were used. In the first PCR protocol using a tms2F1 + tms2R2 primer pair, 617 bp products specific for tumorigenic Agrobacterium strains were amplified. The second PCR protocol, using a tms2F1 + tms2B primer pair, amplified the expected 458 bp products. On the basis of multiplex PCR with primers complementary to chromosomal gene coding for 23S rRNA, the isolated strains were classified as biovar 1 Agrobacterium (A. radiobacter). This is the first report of tumorigenic A. radiobacter on raspberry in Serbia. |
| first_indexed | 2024-12-11T18:52:11Z |
| format | Article |
| id | doaj.art-45903df330b540238eaf3ae1af002965 |
| institution | Directory Open Access Journal |
| issn | 1820-3949 |
| language | English |
| last_indexed | 2024-12-11T18:52:11Z |
| publishDate | 2007-01-01 |
| publisher | Institute of Pesticides and Environmental Protection |
| record_format | Article |
| series | Pesticidi i Fitomedicina |
| spelling | doaj.art-45903df330b540238eaf3ae1af0029652022-12-22T00:54:16ZengInstitute of Pesticides and Environmental ProtectionPesticidi i Fitomedicina1820-39492007-01-01222113119First Report of Tumorigenic Agrobacterium radiobacter on Raspberry in SerbiaSvetlana MilijaševićVeljko GavrilovićSvetlana ŽivkovićNenad TrkuljaJoanna PulawskaDuring the spring of 2003, gall symptoms on the roots and crowns of young raspberry plants cv. Vilamette were observed near Valjevo. Phytopathogenic bacteria were isolated from diseased plant samples. Based on the pathogenic, morphological, differential biochemicaland physiological characteristics, the isolated strains were identified as tumorigenic Agrobacterium radiobacter (biovar 1 Agrobacterium). In order to confirm the identity of isolated strains by polymerase chain reaction (PCR) primers complementary to tms2 genelocated on the Ti plasmid were used. In the first PCR protocol using a tms2F1 + tms2R2 primer pair, 617 bp products specific for tumorigenic Agrobacterium strains were amplified. The second PCR protocol, using a tms2F1 + tms2B primer pair, amplified the expected 458 bp products. On the basis of multiplex PCR with primers complementary to chromosomal gene coding for 23S rRNA, the isolated strains were classified as biovar 1 Agrobacterium (A. radiobacter). This is the first report of tumorigenic A. radiobacter on raspberry in Serbia.http://www.pesting.org.rs/download.php/documents/22-2/22-2%20113-119.pdfTumorigenic AgrobacteriumCrown gallRaspberryIdentificationPCR |
| spellingShingle | Svetlana Milijašević Veljko Gavrilović Svetlana Živković Nenad Trkulja Joanna Pulawska First Report of Tumorigenic Agrobacterium radiobacter on Raspberry in Serbia Pesticidi i Fitomedicina Tumorigenic Agrobacterium Crown gall Raspberry Identification PCR |
| title | First Report of Tumorigenic Agrobacterium radiobacter on Raspberry in Serbia |
| title_full | First Report of Tumorigenic Agrobacterium radiobacter on Raspberry in Serbia |
| title_fullStr | First Report of Tumorigenic Agrobacterium radiobacter on Raspberry in Serbia |
| title_full_unstemmed | First Report of Tumorigenic Agrobacterium radiobacter on Raspberry in Serbia |
| title_short | First Report of Tumorigenic Agrobacterium radiobacter on Raspberry in Serbia |
| title_sort | first report of tumorigenic agrobacterium radiobacter on raspberry in serbia |
| topic | Tumorigenic Agrobacterium Crown gall Raspberry Identification PCR |
| url | http://www.pesting.org.rs/download.php/documents/22-2/22-2%20113-119.pdf |
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