High-resolution harmonics ultrasound imaging for non-invasive characterization of wound healing in a pre-clinical swine model.
This work represents the first study employing non-invasive high-resolution harmonic ultrasound imaging to longitudinally characterize skin wound healing. Burn wounds (day 0-42), on the dorsum of a domestic Yorkshire white pig were studied non-invasively using tandem digital planimetry, laser speckl...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4370665?pdf=render |
| _version_ | 1811209135193063424 |
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| author | Surya C Gnyawali Kasturi G Barki Shomita S Mathew-Steiner Sriteja Dixith Daniel Vanzant Jayne Kim Jennifer L Dickerson Soma Datta Heather Powell Sashwati Roy Valerie Bergdall Chandan K Sen |
| author_facet | Surya C Gnyawali Kasturi G Barki Shomita S Mathew-Steiner Sriteja Dixith Daniel Vanzant Jayne Kim Jennifer L Dickerson Soma Datta Heather Powell Sashwati Roy Valerie Bergdall Chandan K Sen |
| author_sort | Surya C Gnyawali |
| collection | DOAJ |
| description | This work represents the first study employing non-invasive high-resolution harmonic ultrasound imaging to longitudinally characterize skin wound healing. Burn wounds (day 0-42), on the dorsum of a domestic Yorkshire white pig were studied non-invasively using tandem digital planimetry, laser speckle imaging and dual mode (B and Doppler) ultrasound imaging. Wound depth, as measured by B-mode imaging, progressively increased until day 21 and decreased thereafter. Initially, blood flow at the wound edge increased up to day 14 and subsequently regressed to baseline levels by day 21, when the wound was more than 90% closed. Coinciding with regression of blood flow at the wound edge, there was an increase in blood flow in the wound bed. This was observed to regress by day 42. Such changes in wound angiogenesis were corroborated histologically. Gated Doppler imaging quantitated the pulse pressure of the primary feeder artery supplying the wound site. This pulse pressure markedly increased with a bimodal pattern following wounding connecting it to the induction of wound angiogenesis. Finally, ultrasound elastography measured tissue stiffness and visualized growth of new tissue over time. These studies have elegantly captured the physiological sequence of events during the process of wound healing, much of which is anticipated based on certain dynamics in play, to provide the framework for future studies on molecular mechanisms driving these processes. We conclude that the tandem use of non-invasive imaging technologies has the power to provide unprecedented insight into the dynamics of the healing skin tissue. |
| first_indexed | 2024-04-12T04:33:19Z |
| format | Article |
| id | doaj.art-4592d67886ab484db796c84f4bc2ee69 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T04:33:19Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-4592d67886ab484db796c84f4bc2ee692022-12-22T03:47:52ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01103e012232710.1371/journal.pone.0122327High-resolution harmonics ultrasound imaging for non-invasive characterization of wound healing in a pre-clinical swine model.Surya C GnyawaliKasturi G BarkiShomita S Mathew-SteinerSriteja DixithDaniel VanzantJayne KimJennifer L DickersonSoma DattaHeather PowellSashwati RoyValerie BergdallChandan K SenThis work represents the first study employing non-invasive high-resolution harmonic ultrasound imaging to longitudinally characterize skin wound healing. Burn wounds (day 0-42), on the dorsum of a domestic Yorkshire white pig were studied non-invasively using tandem digital planimetry, laser speckle imaging and dual mode (B and Doppler) ultrasound imaging. Wound depth, as measured by B-mode imaging, progressively increased until day 21 and decreased thereafter. Initially, blood flow at the wound edge increased up to day 14 and subsequently regressed to baseline levels by day 21, when the wound was more than 90% closed. Coinciding with regression of blood flow at the wound edge, there was an increase in blood flow in the wound bed. This was observed to regress by day 42. Such changes in wound angiogenesis were corroborated histologically. Gated Doppler imaging quantitated the pulse pressure of the primary feeder artery supplying the wound site. This pulse pressure markedly increased with a bimodal pattern following wounding connecting it to the induction of wound angiogenesis. Finally, ultrasound elastography measured tissue stiffness and visualized growth of new tissue over time. These studies have elegantly captured the physiological sequence of events during the process of wound healing, much of which is anticipated based on certain dynamics in play, to provide the framework for future studies on molecular mechanisms driving these processes. We conclude that the tandem use of non-invasive imaging technologies has the power to provide unprecedented insight into the dynamics of the healing skin tissue.http://europepmc.org/articles/PMC4370665?pdf=render |
| spellingShingle | Surya C Gnyawali Kasturi G Barki Shomita S Mathew-Steiner Sriteja Dixith Daniel Vanzant Jayne Kim Jennifer L Dickerson Soma Datta Heather Powell Sashwati Roy Valerie Bergdall Chandan K Sen High-resolution harmonics ultrasound imaging for non-invasive characterization of wound healing in a pre-clinical swine model. PLoS ONE |
| title | High-resolution harmonics ultrasound imaging for non-invasive characterization of wound healing in a pre-clinical swine model. |
| title_full | High-resolution harmonics ultrasound imaging for non-invasive characterization of wound healing in a pre-clinical swine model. |
| title_fullStr | High-resolution harmonics ultrasound imaging for non-invasive characterization of wound healing in a pre-clinical swine model. |
| title_full_unstemmed | High-resolution harmonics ultrasound imaging for non-invasive characterization of wound healing in a pre-clinical swine model. |
| title_short | High-resolution harmonics ultrasound imaging for non-invasive characterization of wound healing in a pre-clinical swine model. |
| title_sort | high resolution harmonics ultrasound imaging for non invasive characterization of wound healing in a pre clinical swine model |
| url | http://europepmc.org/articles/PMC4370665?pdf=render |
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