Transcriptomics reveals the molecular mechanism underlying kiwifruit expansion following forchlorfenuron (CPPU) treatment
Kiwifruit is an economically and nutritionally valuable fruit. Forchlorfenuron (CPPU) is widely used to improve fruit set and yield of kiwifruit. However, the mechanism through which CPPU regulates fruit enlargement remains unclear. Physiological and RNA-seq data revealed that the greatest contribut...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Maximum Academic Press
2022-01-01
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| Series: | Fruit Research |
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| Online Access: | https://www.maxapress.com/article/doi/10.48130/FruRes-2022-0004 |
| _version_ | 1797293157917917184 |
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| author | Xingyu Chen Lingcheng Zhu Jianyu Song Jing Zhang Mingjun Li |
| author_facet | Xingyu Chen Lingcheng Zhu Jianyu Song Jing Zhang Mingjun Li |
| author_sort | Xingyu Chen |
| collection | DOAJ |
| description | Kiwifruit is an economically and nutritionally valuable fruit. Forchlorfenuron (CPPU) is widely used to improve fruit set and yield of kiwifruit. However, the mechanism through which CPPU regulates fruit enlargement remains unclear. Physiological and RNA-seq data revealed that the greatest contribution of CPPU treatment to fruit growth occurred at 28 to 42 d after bloom (DAB). CPPU application accelerated sugar metabolism by increasing the content of sucrose through increasing the transcripts of genes such as SUSY, HK, and FK in sucrose metabolism. In the early stages of fruit development, up-regulation of the starch synthesis gene AGPase and the decomposition gene β-AMY indicated the occurrence of starch turnover. After CPPU treatment, genes related to the synthesis and signal transduction of auxin and gibberellins were down-regulated. During the early fruit developmental stage, the cytokinin content and the expression of signal transduction genes were increased. In addition, the expression of cyclin genes, related to cell division, was up-regulated within three weeks after CPPU treatment. This indicates that CPPU treatment promoted cell division during the fastest growth stage, which would lead to an increase in cell number and further increases in fruit size. |
| first_indexed | 2024-03-07T20:08:49Z |
| format | Article |
| id | doaj.art-4593a9c12c8c4bd39bc64185e9d5d9ba |
| institution | Directory Open Access Journal |
| issn | 2769-4615 |
| language | English |
| last_indexed | 2024-03-07T20:08:49Z |
| publishDate | 2022-01-01 |
| publisher | Maximum Academic Press |
| record_format | Article |
| series | Fruit Research |
| spelling | doaj.art-4593a9c12c8c4bd39bc64185e9d5d9ba2024-02-28T03:19:34ZengMaximum Academic PressFruit Research2769-46152022-01-012111010.48130/FruRes-2022-0004FruRes-2022-0004Transcriptomics reveals the molecular mechanism underlying kiwifruit expansion following forchlorfenuron (CPPU) treatmentXingyu Chen0Lingcheng Zhu1Jianyu Song2Jing Zhang3Mingjun Li4State Key Laboratory of Crop Stress Biology for Arid Areas/College of Horticulture/Shaanxi Key Laboratory of Apple, Northwest A&F University, Yangling 712100, Shaanxi, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas/College of Horticulture/Shaanxi Key Laboratory of Apple, Northwest A&F University, Yangling 712100, Shaanxi, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas/College of Horticulture/Shaanxi Key Laboratory of Apple, Northwest A&F University, Yangling 712100, Shaanxi, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas/College of Horticulture/Shaanxi Key Laboratory of Apple, Northwest A&F University, Yangling 712100, Shaanxi, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas/College of Horticulture/Shaanxi Key Laboratory of Apple, Northwest A&F University, Yangling 712100, Shaanxi, ChinaKiwifruit is an economically and nutritionally valuable fruit. Forchlorfenuron (CPPU) is widely used to improve fruit set and yield of kiwifruit. However, the mechanism through which CPPU regulates fruit enlargement remains unclear. Physiological and RNA-seq data revealed that the greatest contribution of CPPU treatment to fruit growth occurred at 28 to 42 d after bloom (DAB). CPPU application accelerated sugar metabolism by increasing the content of sucrose through increasing the transcripts of genes such as SUSY, HK, and FK in sucrose metabolism. In the early stages of fruit development, up-regulation of the starch synthesis gene AGPase and the decomposition gene β-AMY indicated the occurrence of starch turnover. After CPPU treatment, genes related to the synthesis and signal transduction of auxin and gibberellins were down-regulated. During the early fruit developmental stage, the cytokinin content and the expression of signal transduction genes were increased. In addition, the expression of cyclin genes, related to cell division, was up-regulated within three weeks after CPPU treatment. This indicates that CPPU treatment promoted cell division during the fastest growth stage, which would lead to an increase in cell number and further increases in fruit size.https://www.maxapress.com/article/doi/10.48130/FruRes-2022-0004kiwifruitforchlorfenuronfruit developmentsugar metabolismhormone. |
| spellingShingle | Xingyu Chen Lingcheng Zhu Jianyu Song Jing Zhang Mingjun Li Transcriptomics reveals the molecular mechanism underlying kiwifruit expansion following forchlorfenuron (CPPU) treatment Fruit Research kiwifruit forchlorfenuron fruit development sugar metabolism hormone. |
| title | Transcriptomics reveals the molecular mechanism underlying kiwifruit expansion following forchlorfenuron (CPPU) treatment |
| title_full | Transcriptomics reveals the molecular mechanism underlying kiwifruit expansion following forchlorfenuron (CPPU) treatment |
| title_fullStr | Transcriptomics reveals the molecular mechanism underlying kiwifruit expansion following forchlorfenuron (CPPU) treatment |
| title_full_unstemmed | Transcriptomics reveals the molecular mechanism underlying kiwifruit expansion following forchlorfenuron (CPPU) treatment |
| title_short | Transcriptomics reveals the molecular mechanism underlying kiwifruit expansion following forchlorfenuron (CPPU) treatment |
| title_sort | transcriptomics reveals the molecular mechanism underlying kiwifruit expansion following forchlorfenuron cppu treatment |
| topic | kiwifruit forchlorfenuron fruit development sugar metabolism hormone. |
| url | https://www.maxapress.com/article/doi/10.48130/FruRes-2022-0004 |
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