Anti-inflammatory and antioxidant properties of Sargassum polycystum ethyl acetate extract from Indonesia
Introduction: Inflammatory diseases, including asthma, osteoarthritis, rheumatoid arthritis, and cancer persist with relatively high incidence, which highlights the need to explore natural alternatives such as the bioactive substances of brown algae (Sargassum polycystum). Certain substances, such a...
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| Format: | Article |
| Language: | English |
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Shahrekord University of Medical Sciences
2023-06-01
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| Series: | Journal of HerbMed Pharmacology |
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| Online Access: | http://herbmedpharmacol.com/PDF/jhp-12-407.pdf |
| _version_ | 1827636133192794112 |
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| author | Erlia Anggrainy Sianipar Shannen Ivanka Gunardi |
| author_facet | Erlia Anggrainy Sianipar Shannen Ivanka Gunardi |
| author_sort | Erlia Anggrainy Sianipar |
| collection | DOAJ |
| description | Introduction: Inflammatory diseases, including asthma, osteoarthritis, rheumatoid arthritis, and cancer persist with relatively high incidence, which highlights the need to explore natural alternatives such as the bioactive substances of brown algae (Sargassum polycystum). Certain substances, such as phenolics and flavonoids, have been demonstrated to have an association with antioxidant activity because of their capacity to fend off free radicals, which cause inflammation. The purpose of this study was to look into the inflammatory inhibition and antioxidant activity of an ethyl acetate extract of S. polycystum from Indonesia. Methods: After maceration in ethyl acetate (1:4 w/v) for 24 hours, the total phenolic and flavonoid content of S. polycystum extract was measured using the colorimetric method. The antioxidant activity was tested using 1,1-diphenyl-2-picrylhydrazyl (DPPH), and the models of paw edema induced by carrageenan were used to assess inflammatory inhibition activity. Results: The findings revealed that S. polycystum had a weak antioxidant with a half-maximal inhibitory concentration (IC50) value of 605.24 ± 26.53 g/mL. Furthermore, it had 23.509 ± 0.109 mg gallic acid equivalents/gram (GAE/g) and 15.186 ± 0.046 mg quercetin equivalents/ gram (QE/g) of total phenolic and flavonoid compounds, respectively. At 200, 400, and 800 mg/ kg BW doses, S. polycystum had inflammatory inhibition percentages of 53.80 ± 4.68%, 62.35 ± 4.05%, and 60.90 ± 2.88%, respectively. Conclusion: Sargassum polycystum ethyl acetate extract inhibited inflammation. However, further study is needed to examine the safety, identification, separation of the relevant substances involved, and the precise mechanism of action. |
| first_indexed | 2024-03-09T15:38:50Z |
| format | Article |
| id | doaj.art-4593e22aa03f4174ae04549f1c48f167 |
| institution | Directory Open Access Journal |
| issn | 2345-5004 |
| language | English |
| last_indexed | 2024-03-09T15:38:50Z |
| publishDate | 2023-06-01 |
| publisher | Shahrekord University of Medical Sciences |
| record_format | Article |
| series | Journal of HerbMed Pharmacology |
| spelling | doaj.art-4593e22aa03f4174ae04549f1c48f1672023-11-25T08:27:27ZengShahrekord University of Medical SciencesJournal of HerbMed Pharmacology2345-50042023-06-0112340741210.34172/jhp.2023.44jhp-44840Anti-inflammatory and antioxidant properties of Sargassum polycystum ethyl acetate extract from IndonesiaErlia Anggrainy Sianipar0Shannen Ivanka Gunardi1Department of Pharmacy, School of Medicine and Health Science, Atma Jaya Catholic University of Indonesia, Pluit Raya 2, Jakarta Utara, DKI Jakarta 14440, IndonesiaDepartment of Pharmacy, School of Medicine and Health Science, Atma Jaya Catholic University of Indonesia, Pluit Raya 2, Jakarta Utara, DKI Jakarta 14440, IndonesiaIntroduction: Inflammatory diseases, including asthma, osteoarthritis, rheumatoid arthritis, and cancer persist with relatively high incidence, which highlights the need to explore natural alternatives such as the bioactive substances of brown algae (Sargassum polycystum). Certain substances, such as phenolics and flavonoids, have been demonstrated to have an association with antioxidant activity because of their capacity to fend off free radicals, which cause inflammation. The purpose of this study was to look into the inflammatory inhibition and antioxidant activity of an ethyl acetate extract of S. polycystum from Indonesia. Methods: After maceration in ethyl acetate (1:4 w/v) for 24 hours, the total phenolic and flavonoid content of S. polycystum extract was measured using the colorimetric method. The antioxidant activity was tested using 1,1-diphenyl-2-picrylhydrazyl (DPPH), and the models of paw edema induced by carrageenan were used to assess inflammatory inhibition activity. Results: The findings revealed that S. polycystum had a weak antioxidant with a half-maximal inhibitory concentration (IC50) value of 605.24 ± 26.53 g/mL. Furthermore, it had 23.509 ± 0.109 mg gallic acid equivalents/gram (GAE/g) and 15.186 ± 0.046 mg quercetin equivalents/ gram (QE/g) of total phenolic and flavonoid compounds, respectively. At 200, 400, and 800 mg/ kg BW doses, S. polycystum had inflammatory inhibition percentages of 53.80 ± 4.68%, 62.35 ± 4.05%, and 60.90 ± 2.88%, respectively. Conclusion: Sargassum polycystum ethyl acetate extract inhibited inflammation. However, further study is needed to examine the safety, identification, separation of the relevant substances involved, and the precise mechanism of action.http://herbmedpharmacol.com/PDF/jhp-12-407.pdfsargassumfree radicalsflavonoidsphenolicsinflammation |
| spellingShingle | Erlia Anggrainy Sianipar Shannen Ivanka Gunardi Anti-inflammatory and antioxidant properties of Sargassum polycystum ethyl acetate extract from Indonesia Journal of HerbMed Pharmacology sargassum free radicals flavonoids phenolics inflammation |
| title | Anti-inflammatory and antioxidant properties of Sargassum polycystum ethyl acetate extract from Indonesia |
| title_full | Anti-inflammatory and antioxidant properties of Sargassum polycystum ethyl acetate extract from Indonesia |
| title_fullStr | Anti-inflammatory and antioxidant properties of Sargassum polycystum ethyl acetate extract from Indonesia |
| title_full_unstemmed | Anti-inflammatory and antioxidant properties of Sargassum polycystum ethyl acetate extract from Indonesia |
| title_short | Anti-inflammatory and antioxidant properties of Sargassum polycystum ethyl acetate extract from Indonesia |
| title_sort | anti inflammatory and antioxidant properties of sargassum polycystum ethyl acetate extract from indonesia |
| topic | sargassum free radicals flavonoids phenolics inflammation |
| url | http://herbmedpharmacol.com/PDF/jhp-12-407.pdf |
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