Acetylation of p65 at lysine 314 is important for late NF-κB-dependent gene expression
<p>Abstract</p> <p>Background</p> <p>NF-κB regulates the expression of a large number of target genes involved in the immune and inflammatory response, apoptosis, cell proliferation, differentiation and survival. We have earlier reported that p65, a subunit of NF-κB, is...
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| Format: | Article |
| Language: | English |
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BMC
2010-01-01
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| Series: | BMC Genomics |
| Online Access: | http://www.biomedcentral.com/1471-2164/11/22 |
| _version_ | 1818750899319734272 |
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| author | Hottiger Michael O Fey Monika Rothgiesser Karin M |
| author_facet | Hottiger Michael O Fey Monika Rothgiesser Karin M |
| author_sort | Hottiger Michael O |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>NF-κB regulates the expression of a large number of target genes involved in the immune and inflammatory response, apoptosis, cell proliferation, differentiation and survival. We have earlier reported that p65, a subunit of NF-κB, is acetylated <it>in vitro </it>and <it>in vivo </it>at three different lysines (K310, K314 and K315) by the histone acetyltransferase p300.</p> <p>Results</p> <p>In this study, we describe that site-specific mutation of p65 at lysines 314 and 315 enhances gene expression of a subset of NF-κB target genes including <it>Mmp10 </it>and <it>Mmp13</it>. Increased gene expression was mainly observed three hours after TNFα stimulation. Chromatin immunoprecipitation (ChIP) experiments with an antibody raised against acetylated lysine 314 revealed that chromatin-bound p65 is indeed acetylated at lysine 314.</p> <p>Conclusions</p> <p>Together, our results establish acetylation of K314 as an important regulatory modification of p65 and subsequently of NF-κB-dependent gene expression.</p> |
| first_indexed | 2024-12-18T04:27:00Z |
| format | Article |
| id | doaj.art-459596793ff74ccf80090c0546137ec4 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-18T04:27:00Z |
| publishDate | 2010-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-459596793ff74ccf80090c0546137ec42022-12-21T21:21:04ZengBMCBMC Genomics1471-21642010-01-011112210.1186/1471-2164-11-22Acetylation of p65 at lysine 314 is important for late NF-κB-dependent gene expressionHottiger Michael OFey MonikaRothgiesser Karin M<p>Abstract</p> <p>Background</p> <p>NF-κB regulates the expression of a large number of target genes involved in the immune and inflammatory response, apoptosis, cell proliferation, differentiation and survival. We have earlier reported that p65, a subunit of NF-κB, is acetylated <it>in vitro </it>and <it>in vivo </it>at three different lysines (K310, K314 and K315) by the histone acetyltransferase p300.</p> <p>Results</p> <p>In this study, we describe that site-specific mutation of p65 at lysines 314 and 315 enhances gene expression of a subset of NF-κB target genes including <it>Mmp10 </it>and <it>Mmp13</it>. Increased gene expression was mainly observed three hours after TNFα stimulation. Chromatin immunoprecipitation (ChIP) experiments with an antibody raised against acetylated lysine 314 revealed that chromatin-bound p65 is indeed acetylated at lysine 314.</p> <p>Conclusions</p> <p>Together, our results establish acetylation of K314 as an important regulatory modification of p65 and subsequently of NF-κB-dependent gene expression.</p>http://www.biomedcentral.com/1471-2164/11/22 |
| spellingShingle | Hottiger Michael O Fey Monika Rothgiesser Karin M Acetylation of p65 at lysine 314 is important for late NF-κB-dependent gene expression BMC Genomics |
| title | Acetylation of p65 at lysine 314 is important for late NF-κB-dependent gene expression |
| title_full | Acetylation of p65 at lysine 314 is important for late NF-κB-dependent gene expression |
| title_fullStr | Acetylation of p65 at lysine 314 is important for late NF-κB-dependent gene expression |
| title_full_unstemmed | Acetylation of p65 at lysine 314 is important for late NF-κB-dependent gene expression |
| title_short | Acetylation of p65 at lysine 314 is important for late NF-κB-dependent gene expression |
| title_sort | acetylation of p65 at lysine 314 is important for late nf κb dependent gene expression |
| url | http://www.biomedcentral.com/1471-2164/11/22 |
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