Ruthenium-Anchored Carbon Sphere-Customized Sensor for the Selective Amperometric Detection of Melatonin
Melatonin (MT), a pineal gland hormone, regulates the sleep/wake cycle and is a potential biomarker for neurodegenerative disorders, depression, hypertension, and several cancers, including prostate cancer and hepatocarcinoma. The amperometric detection of MT was achieved using a sensor customized w...
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| Format: | Article |
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MDPI AG
2023-10-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/13/10/936 |
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| author | Sivaguru Jayaraman Thenmozhi Rajarathinam Hyeon-Geun Jang Dinakaran Thirumalai Jaewon Lee Hyun-Jong Paik Seung-Cheol Chang |
| author_facet | Sivaguru Jayaraman Thenmozhi Rajarathinam Hyeon-Geun Jang Dinakaran Thirumalai Jaewon Lee Hyun-Jong Paik Seung-Cheol Chang |
| author_sort | Sivaguru Jayaraman |
| collection | DOAJ |
| description | Melatonin (MT), a pineal gland hormone, regulates the sleep/wake cycle and is a potential biomarker for neurodegenerative disorders, depression, hypertension, and several cancers, including prostate cancer and hepatocarcinoma. The amperometric detection of MT was achieved using a sensor customized with ruthenium-incorporated carbon spheres (Ru–CS), possessing C- and O-rich catalytically active Ru surfaces. The non-covalent interactions and ion–molecule adducts between Ru and CS favor the formation of heterojunctions at the sensor–analyte interface, thus accelerating the reactions towards MT. The Ru–CS/Screen-printed carbon electrode (SPCE) sensor demonstrated the outstanding electrocatalytic oxidation of MT owing to its high surface area and heterogeneous rate constants and afforded a lower detection limit (0.27 μM), high sensitivity (0.85 μA μM <sup>−1</sup> cm<sup>−2</sup>), and excellent selectivity for MT with the co-existence of crucial neurotransmitters, including norepinephrine, epinephrine, dopamine, and serotonin. High concentrations of active biomolecules, such as ascorbic acid and tyrosine, did not interfere with MT detection. The practical feasibility of the sensor for MT detection in pharmaceutical samples was demonstrated, comparable to the data provided on the product labels. The developed amperometric sensor is highly suitable for the quality control of medicines because of its low cost, simplicity, small sample size, speed of analysis, and potential for automation. |
| first_indexed | 2024-03-10T21:23:23Z |
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| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T21:23:23Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-4596c6f987674686923531de56b6be6f2023-11-19T15:51:34ZengMDPI AGBiosensors2079-63742023-10-01131093610.3390/bios13100936Ruthenium-Anchored Carbon Sphere-Customized Sensor for the Selective Amperometric Detection of MelatoninSivaguru Jayaraman0Thenmozhi Rajarathinam1Hyeon-Geun Jang2Dinakaran Thirumalai3Jaewon Lee4Hyun-Jong Paik5Seung-Cheol Chang6Department of Cogno-Mechatronics Engineering, College of Nanoscience and Nanotechnology, Pusan National University, Busan 46241, Republic of KoreaDepartment of Cogno-Mechatronics Engineering, College of Nanoscience and Nanotechnology, Pusan National University, Busan 46241, Republic of KoreaDepartment of Cogno-Mechatronics Engineering, College of Nanoscience and Nanotechnology, Pusan National University, Busan 46241, Republic of KoreaBIT Convergence-Based Innovative Drug Development Targeting Metainflammation, Pusan National University, Busan 46241, Republic of KoreaBIT Convergence-Based Innovative Drug Development Targeting Metainflammation, Pusan National University, Busan 46241, Republic of KoreaDepartment of Polymer Science and Engineering, Pusan National University, Busan 46241, Republic of KoreaDepartment of Cogno-Mechatronics Engineering, College of Nanoscience and Nanotechnology, Pusan National University, Busan 46241, Republic of KoreaMelatonin (MT), a pineal gland hormone, regulates the sleep/wake cycle and is a potential biomarker for neurodegenerative disorders, depression, hypertension, and several cancers, including prostate cancer and hepatocarcinoma. The amperometric detection of MT was achieved using a sensor customized with ruthenium-incorporated carbon spheres (Ru–CS), possessing C- and O-rich catalytically active Ru surfaces. The non-covalent interactions and ion–molecule adducts between Ru and CS favor the formation of heterojunctions at the sensor–analyte interface, thus accelerating the reactions towards MT. The Ru–CS/Screen-printed carbon electrode (SPCE) sensor demonstrated the outstanding electrocatalytic oxidation of MT owing to its high surface area and heterogeneous rate constants and afforded a lower detection limit (0.27 μM), high sensitivity (0.85 μA μM <sup>−1</sup> cm<sup>−2</sup>), and excellent selectivity for MT with the co-existence of crucial neurotransmitters, including norepinephrine, epinephrine, dopamine, and serotonin. High concentrations of active biomolecules, such as ascorbic acid and tyrosine, did not interfere with MT detection. The practical feasibility of the sensor for MT detection in pharmaceutical samples was demonstrated, comparable to the data provided on the product labels. The developed amperometric sensor is highly suitable for the quality control of medicines because of its low cost, simplicity, small sample size, speed of analysis, and potential for automation.https://www.mdpi.com/2079-6374/13/10/936melatoninamperometric sensorrutheniumcarbon sphereslower detection limitpharmaceutical samples |
| spellingShingle | Sivaguru Jayaraman Thenmozhi Rajarathinam Hyeon-Geun Jang Dinakaran Thirumalai Jaewon Lee Hyun-Jong Paik Seung-Cheol Chang Ruthenium-Anchored Carbon Sphere-Customized Sensor for the Selective Amperometric Detection of Melatonin Biosensors melatonin amperometric sensor ruthenium carbon spheres lower detection limit pharmaceutical samples |
| title | Ruthenium-Anchored Carbon Sphere-Customized Sensor for the Selective Amperometric Detection of Melatonin |
| title_full | Ruthenium-Anchored Carbon Sphere-Customized Sensor for the Selective Amperometric Detection of Melatonin |
| title_fullStr | Ruthenium-Anchored Carbon Sphere-Customized Sensor for the Selective Amperometric Detection of Melatonin |
| title_full_unstemmed | Ruthenium-Anchored Carbon Sphere-Customized Sensor for the Selective Amperometric Detection of Melatonin |
| title_short | Ruthenium-Anchored Carbon Sphere-Customized Sensor for the Selective Amperometric Detection of Melatonin |
| title_sort | ruthenium anchored carbon sphere customized sensor for the selective amperometric detection of melatonin |
| topic | melatonin amperometric sensor ruthenium carbon spheres lower detection limit pharmaceutical samples |
| url | https://www.mdpi.com/2079-6374/13/10/936 |
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