Development of a Synthetic Synovial Fluid for Tribological Testing
Wear tests of joint prostheses are usually performed using bovine calf serum. The results from different laboratories are hardly ever comparable as, for example, the protein concentration and the protein composition of the serum-based test liquids vary. In addition, the viscosity of these test liqui...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-12-01
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| Series: | Lubricants |
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| Online Access: | http://www.mdpi.com/2075-4442/3/4/664 |
| _version_ | 1798005130786766848 |
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| author | Emely Lea Bortel Baptiste Charbonnier Roman Heuberger |
| author_facet | Emely Lea Bortel Baptiste Charbonnier Roman Heuberger |
| author_sort | Emely Lea Bortel |
| collection | DOAJ |
| description | Wear tests of joint prostheses are usually performed using bovine calf serum. The results from different laboratories are hardly ever comparable as, for example, the protein concentration and the protein composition of the serum-based test liquids vary. In addition, the viscosity of these test liquids is similar to that of water and does not match the more viscous synovial fluid. The present work was aimed at developing a synthetic synovial fluid as an alternative to the existing test liquids. Improved consistency and reproducibility of results at a similar price were required. Hyaluronic acid (HA), the lyophilized proteins bovine serum albumin (BSA) and immunoglobulin G (IgG), the phospholipid lecithin (PL) and salts were applied in a stepwise approach to replace the actually used test liquid based on newborn calf serum. The in vitro results obtained with ultra-high-molecular-weight polyethylene (UHMWPE) pins sliding against CoCrMo discs revealed that the developed synthetic synovial fluid fulfils the set requirements: increase of viscosity, reasonable cost, improved consistency and wear particles which resemble the ones found in vivo. The developed synthetic synovial fluid with 3 g/L HA, 19 g/L BSA, 11 g/L IgG, 0.1 g/L PL and Ringer solution is a more realistic alternative to the used serum-based test liquid. |
| first_indexed | 2024-04-11T12:35:22Z |
| format | Article |
| id | doaj.art-45980d8078ca449a87ce994f2e62f6fe |
| institution | Directory Open Access Journal |
| issn | 2075-4442 |
| language | English |
| last_indexed | 2024-04-11T12:35:22Z |
| publishDate | 2015-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Lubricants |
| spelling | doaj.art-45980d8078ca449a87ce994f2e62f6fe2022-12-22T04:23:39ZengMDPI AGLubricants2075-44422015-12-013466468610.3390/lubricants3040664lubricants3040664Development of a Synthetic Synovial Fluid for Tribological TestingEmely Lea Bortel0Baptiste Charbonnier1Roman Heuberger2RMS Foundation, Bischmattstrasse 12, CH-4500 Bettlach, SwitzerlandRMS Foundation, Bischmattstrasse 12, CH-4500 Bettlach, SwitzerlandRMS Foundation, Bischmattstrasse 12, CH-4500 Bettlach, SwitzerlandWear tests of joint prostheses are usually performed using bovine calf serum. The results from different laboratories are hardly ever comparable as, for example, the protein concentration and the protein composition of the serum-based test liquids vary. In addition, the viscosity of these test liquids is similar to that of water and does not match the more viscous synovial fluid. The present work was aimed at developing a synthetic synovial fluid as an alternative to the existing test liquids. Improved consistency and reproducibility of results at a similar price were required. Hyaluronic acid (HA), the lyophilized proteins bovine serum albumin (BSA) and immunoglobulin G (IgG), the phospholipid lecithin (PL) and salts were applied in a stepwise approach to replace the actually used test liquid based on newborn calf serum. The in vitro results obtained with ultra-high-molecular-weight polyethylene (UHMWPE) pins sliding against CoCrMo discs revealed that the developed synthetic synovial fluid fulfils the set requirements: increase of viscosity, reasonable cost, improved consistency and wear particles which resemble the ones found in vivo. The developed synthetic synovial fluid with 3 g/L HA, 19 g/L BSA, 11 g/L IgG, 0.1 g/L PL and Ringer solution is a more realistic alternative to the used serum-based test liquid.http://www.mdpi.com/2075-4442/3/4/664synthetic synovial fluidviscositytribologyprosthesis testingUHMWPEpolyethyleneCoCrMo |
| spellingShingle | Emely Lea Bortel Baptiste Charbonnier Roman Heuberger Development of a Synthetic Synovial Fluid for Tribological Testing Lubricants synthetic synovial fluid viscosity tribology prosthesis testing UHMWPE polyethylene CoCrMo |
| title | Development of a Synthetic Synovial Fluid for Tribological Testing |
| title_full | Development of a Synthetic Synovial Fluid for Tribological Testing |
| title_fullStr | Development of a Synthetic Synovial Fluid for Tribological Testing |
| title_full_unstemmed | Development of a Synthetic Synovial Fluid for Tribological Testing |
| title_short | Development of a Synthetic Synovial Fluid for Tribological Testing |
| title_sort | development of a synthetic synovial fluid for tribological testing |
| topic | synthetic synovial fluid viscosity tribology prosthesis testing UHMWPE polyethylene CoCrMo |
| url | http://www.mdpi.com/2075-4442/3/4/664 |
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