Changes in Reactive Oxygen Species, Antioxidants and Carbohydrate Metabolism in Relation to Dormancy Transition and Bud Break in Apple (<i>Malus</i> × <i>domestica</i> Borkh) Cultivars
Understanding the biochemical mechanisms underlying bud dormancy and bloom time regulation in deciduous woody perennials is critical for devising effective strategies to protect these species from spring frost damage. This study investigated the accumulation profiles of carbohydrates, ROS and antiox...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/10/1549 |
| _version_ | 1797515419319271424 |
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| author | Sangeeta Sapkota Jianyang Liu Md Tabibul Islam Sherif M. Sherif |
| author_facet | Sangeeta Sapkota Jianyang Liu Md Tabibul Islam Sherif M. Sherif |
| author_sort | Sangeeta Sapkota |
| collection | DOAJ |
| description | Understanding the biochemical mechanisms underlying bud dormancy and bloom time regulation in deciduous woody perennials is critical for devising effective strategies to protect these species from spring frost damage. This study investigated the accumulation profiles of carbohydrates, ROS and antioxidants during dormancy in ‘Cripps Pink’ and ‘Honeycrisp’, two apple cultivars representing the early and late bloom cultivars, respectively. Our data showed that starch levels generally declined during dormancy, whereas soluble sugars increased. However, the present study did not record significant alternations in the carbohydrate accumulation profiles between the two cultivars that could account for the differences in their bloom dates. On the other hand, H<sub>2</sub>O<sub>2</sub> accumulation patterns revealed an apparent correlation with the dormancy stage and bloom dates in both cultivars; peaking early in the early-blooming cultivar, sustaining high levels for a longer time in the late-blooming cultivars, and fading by the time of bud burst in both cultivars. Also, the redox balance during dormancy appeared to be maintained mainly by catalase and, to a lesser extent, by glutathione (GSH). Overall, the present study concludes that differences in ROS and the bud redox balance could, at least partially, explain the differences in dormancy duration and bloom date among apple cultivars. |
| first_indexed | 2024-03-10T06:45:13Z |
| format | Article |
| id | doaj.art-4599f50d6768486d9cbb80a4a2e12001 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T06:45:13Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-4599f50d6768486d9cbb80a4a2e120012023-11-22T17:15:40ZengMDPI AGAntioxidants2076-39212021-09-011010154910.3390/antiox10101549Changes in Reactive Oxygen Species, Antioxidants and Carbohydrate Metabolism in Relation to Dormancy Transition and Bud Break in Apple (<i>Malus</i> × <i>domestica</i> Borkh) CultivarsSangeeta Sapkota0Jianyang Liu1Md Tabibul Islam2Sherif M. Sherif3Alson H. Smith Jr. Agricultural Research and Extension Center, School of Plant and Environmental Sciences, Virginia Tech, 595 Laurel Grove Road, Winchester, VA 22602, USAAlson H. Smith Jr. Agricultural Research and Extension Center, School of Plant and Environmental Sciences, Virginia Tech, 595 Laurel Grove Road, Winchester, VA 22602, USAAlson H. Smith Jr. Agricultural Research and Extension Center, School of Plant and Environmental Sciences, Virginia Tech, 595 Laurel Grove Road, Winchester, VA 22602, USAAlson H. Smith Jr. Agricultural Research and Extension Center, School of Plant and Environmental Sciences, Virginia Tech, 595 Laurel Grove Road, Winchester, VA 22602, USAUnderstanding the biochemical mechanisms underlying bud dormancy and bloom time regulation in deciduous woody perennials is critical for devising effective strategies to protect these species from spring frost damage. This study investigated the accumulation profiles of carbohydrates, ROS and antioxidants during dormancy in ‘Cripps Pink’ and ‘Honeycrisp’, two apple cultivars representing the early and late bloom cultivars, respectively. Our data showed that starch levels generally declined during dormancy, whereas soluble sugars increased. However, the present study did not record significant alternations in the carbohydrate accumulation profiles between the two cultivars that could account for the differences in their bloom dates. On the other hand, H<sub>2</sub>O<sub>2</sub> accumulation patterns revealed an apparent correlation with the dormancy stage and bloom dates in both cultivars; peaking early in the early-blooming cultivar, sustaining high levels for a longer time in the late-blooming cultivars, and fading by the time of bud burst in both cultivars. Also, the redox balance during dormancy appeared to be maintained mainly by catalase and, to a lesser extent, by glutathione (GSH). Overall, the present study concludes that differences in ROS and the bud redox balance could, at least partially, explain the differences in dormancy duration and bloom date among apple cultivars.https://www.mdpi.com/2076-3921/10/10/1549bud dormancyspring frostbloom delaypome fruitsappleROS |
| spellingShingle | Sangeeta Sapkota Jianyang Liu Md Tabibul Islam Sherif M. Sherif Changes in Reactive Oxygen Species, Antioxidants and Carbohydrate Metabolism in Relation to Dormancy Transition and Bud Break in Apple (<i>Malus</i> × <i>domestica</i> Borkh) Cultivars Antioxidants bud dormancy spring frost bloom delay pome fruits apple ROS |
| title | Changes in Reactive Oxygen Species, Antioxidants and Carbohydrate Metabolism in Relation to Dormancy Transition and Bud Break in Apple (<i>Malus</i> × <i>domestica</i> Borkh) Cultivars |
| title_full | Changes in Reactive Oxygen Species, Antioxidants and Carbohydrate Metabolism in Relation to Dormancy Transition and Bud Break in Apple (<i>Malus</i> × <i>domestica</i> Borkh) Cultivars |
| title_fullStr | Changes in Reactive Oxygen Species, Antioxidants and Carbohydrate Metabolism in Relation to Dormancy Transition and Bud Break in Apple (<i>Malus</i> × <i>domestica</i> Borkh) Cultivars |
| title_full_unstemmed | Changes in Reactive Oxygen Species, Antioxidants and Carbohydrate Metabolism in Relation to Dormancy Transition and Bud Break in Apple (<i>Malus</i> × <i>domestica</i> Borkh) Cultivars |
| title_short | Changes in Reactive Oxygen Species, Antioxidants and Carbohydrate Metabolism in Relation to Dormancy Transition and Bud Break in Apple (<i>Malus</i> × <i>domestica</i> Borkh) Cultivars |
| title_sort | changes in reactive oxygen species antioxidants and carbohydrate metabolism in relation to dormancy transition and bud break in apple i malus i i domestica i borkh cultivars |
| topic | bud dormancy spring frost bloom delay pome fruits apple ROS |
| url | https://www.mdpi.com/2076-3921/10/10/1549 |
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