Genome-Wide Identification and Analysis of Stress Response of Trehalose-6-Phosphate Synthase and Trehalose-6-Phosphate Phosphatase Genes in Quinoa
Saline-alkali stress seriously affects the yield and quality of crops, threatening food security and ecological security. Improving saline-alkali land and increasing effective cultivated land are conducive to sustainable agricultural development. Trehalose, a nonreducing disaccharide, is closely rel...
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MDPI AG
2023-04-01
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| Series: | International Journal of Molecular Sciences |
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| author | Xiaoting Wang Mingyu Wang Yongshun Huang Peng Zhu Guangtao Qian Yiming Zhang Yuqi Liu Jingwen Zhou Lixin Li |
| author_facet | Xiaoting Wang Mingyu Wang Yongshun Huang Peng Zhu Guangtao Qian Yiming Zhang Yuqi Liu Jingwen Zhou Lixin Li |
| author_sort | Xiaoting Wang |
| collection | DOAJ |
| description | Saline-alkali stress seriously affects the yield and quality of crops, threatening food security and ecological security. Improving saline-alkali land and increasing effective cultivated land are conducive to sustainable agricultural development. Trehalose, a nonreducing disaccharide, is closely related to plant growth and development and stress response. Trehalose 6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP) are key enzymes catalyzing trehalose biosynthesis. To elucidate the effects of long-term saline-alkali stress on trehalose synthesis and metabolism, we conducted an integrated transcriptome and metabolome analysis. As a result, 13 <i>TPS</i> and 11 <i>TPP</i> genes were identified in quinoa (<i>Chenopodium quinoa</i> Willd.) and were named <i>CqTPS1-13</i> and <i>CqTPP1-11</i> according to the order of their Gene IDs. Through phylogenetic analysis, the CqTPS family is divided into two classes, and the CqTPP family is divided into three classes. Analyses of physicochemical properties, gene structures, conservative domains and motifs in the proteins, and cis-regulatory elements, as well as evolutionary relationships, indicate that the TPS and TPP family characteristics are highly conserved in quinoa. Transcriptome and metabolome analyses of the sucrose and starch metabolism pathway in leaves undergoing saline-alkali stress indicate that <i>CqTPP</i> and Class II <i>CqTPS</i> genes are involved in the stress response. Moreover, the accumulation of some metabolites and the expression of many regulatory genes in the trehalose biosynthesis pathway changed significantly, suggesting the metabolic process is important for the saline-alkali stress response in quinoa. |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-459d48a725c341e286e9e3d38b39b7a32023-11-17T19:32:55ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-04-01248695010.3390/ijms24086950Genome-Wide Identification and Analysis of Stress Response of Trehalose-6-Phosphate Synthase and Trehalose-6-Phosphate Phosphatase Genes in QuinoaXiaoting Wang0Mingyu Wang1Yongshun Huang2Peng Zhu3Guangtao Qian4Yiming Zhang5Yuqi Liu6Jingwen Zhou7Lixin Li8Key Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin 150040, ChinaKey Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin 150040, ChinaKey Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin 150040, ChinaKey Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin 150040, ChinaKey Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin 150040, ChinaKey Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin 150040, ChinaKey Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin 150040, ChinaKey Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin 150040, ChinaKey Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin 150040, ChinaSaline-alkali stress seriously affects the yield and quality of crops, threatening food security and ecological security. Improving saline-alkali land and increasing effective cultivated land are conducive to sustainable agricultural development. Trehalose, a nonreducing disaccharide, is closely related to plant growth and development and stress response. Trehalose 6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP) are key enzymes catalyzing trehalose biosynthesis. To elucidate the effects of long-term saline-alkali stress on trehalose synthesis and metabolism, we conducted an integrated transcriptome and metabolome analysis. As a result, 13 <i>TPS</i> and 11 <i>TPP</i> genes were identified in quinoa (<i>Chenopodium quinoa</i> Willd.) and were named <i>CqTPS1-13</i> and <i>CqTPP1-11</i> according to the order of their Gene IDs. Through phylogenetic analysis, the CqTPS family is divided into two classes, and the CqTPP family is divided into three classes. Analyses of physicochemical properties, gene structures, conservative domains and motifs in the proteins, and cis-regulatory elements, as well as evolutionary relationships, indicate that the TPS and TPP family characteristics are highly conserved in quinoa. Transcriptome and metabolome analyses of the sucrose and starch metabolism pathway in leaves undergoing saline-alkali stress indicate that <i>CqTPP</i> and Class II <i>CqTPS</i> genes are involved in the stress response. Moreover, the accumulation of some metabolites and the expression of many regulatory genes in the trehalose biosynthesis pathway changed significantly, suggesting the metabolic process is important for the saline-alkali stress response in quinoa.https://www.mdpi.com/1422-0067/24/8/6950trehalose biosynthesissaline-alkali stressquinoa<i>CqTPS</i> and <i>CqTPP</i> gene familiestranscriptomics and metabolomics |
| spellingShingle | Xiaoting Wang Mingyu Wang Yongshun Huang Peng Zhu Guangtao Qian Yiming Zhang Yuqi Liu Jingwen Zhou Lixin Li Genome-Wide Identification and Analysis of Stress Response of Trehalose-6-Phosphate Synthase and Trehalose-6-Phosphate Phosphatase Genes in Quinoa International Journal of Molecular Sciences trehalose biosynthesis saline-alkali stress quinoa <i>CqTPS</i> and <i>CqTPP</i> gene families transcriptomics and metabolomics |
| title | Genome-Wide Identification and Analysis of Stress Response of Trehalose-6-Phosphate Synthase and Trehalose-6-Phosphate Phosphatase Genes in Quinoa |
| title_full | Genome-Wide Identification and Analysis of Stress Response of Trehalose-6-Phosphate Synthase and Trehalose-6-Phosphate Phosphatase Genes in Quinoa |
| title_fullStr | Genome-Wide Identification and Analysis of Stress Response of Trehalose-6-Phosphate Synthase and Trehalose-6-Phosphate Phosphatase Genes in Quinoa |
| title_full_unstemmed | Genome-Wide Identification and Analysis of Stress Response of Trehalose-6-Phosphate Synthase and Trehalose-6-Phosphate Phosphatase Genes in Quinoa |
| title_short | Genome-Wide Identification and Analysis of Stress Response of Trehalose-6-Phosphate Synthase and Trehalose-6-Phosphate Phosphatase Genes in Quinoa |
| title_sort | genome wide identification and analysis of stress response of trehalose 6 phosphate synthase and trehalose 6 phosphate phosphatase genes in quinoa |
| topic | trehalose biosynthesis saline-alkali stress quinoa <i>CqTPS</i> and <i>CqTPP</i> gene families transcriptomics and metabolomics |
| url | https://www.mdpi.com/1422-0067/24/8/6950 |
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