Alginate Gel Encapsulated with Enzybiotics Cocktail Is Effective against Multispecies Biofilms
The development of new and effective antibacterials for pharmaceutical or cosmetic skin care that have a low potential for the emergence and expansion of bacterial resistance is of high demand in scientific and applied research. Great hopes are placed on alternative agents such as bactericidal pepti...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-01-01
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| Series: | Gels |
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| Online Access: | https://www.mdpi.com/2310-2861/10/1/60 |
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| author | Daria V. Vasina Nataliia P. Antonova Elena V. Shidlovskaya Nadezhda A. Kuznetsova Alexander V. Grishin Elizaveta A. Akoulina Ekaterina A. Trusova Anastasiya M. Lendel Elena P. Mazunina Sofia R. Kozlova Andrei A. Dudun Anton P. Bonartsev Vladimir G. Lunin Vladimir A. Gushchin |
| author_facet | Daria V. Vasina Nataliia P. Antonova Elena V. Shidlovskaya Nadezhda A. Kuznetsova Alexander V. Grishin Elizaveta A. Akoulina Ekaterina A. Trusova Anastasiya M. Lendel Elena P. Mazunina Sofia R. Kozlova Andrei A. Dudun Anton P. Bonartsev Vladimir G. Lunin Vladimir A. Gushchin |
| author_sort | Daria V. Vasina |
| collection | DOAJ |
| description | The development of new and effective antibacterials for pharmaceutical or cosmetic skin care that have a low potential for the emergence and expansion of bacterial resistance is of high demand in scientific and applied research. Great hopes are placed on alternative agents such as bactericidal peptidoglycan hydrolases, depolymerases, etc. Enzybiotic-based preparations are being studied for the treatment of various infections and, among others, can be used as topical formulations and dressings with protein-polysaccharide complexes. Here, we investigate the antibiofilm properties of a novel enzybiotic cocktail of phage endolysin LysSi3 and bacteriocin lysostaphin, formulated in the alginate gel matrix and its ability to control the opportunistic skin-colonizing bacteria <i>Staphylococcus aureus</i>, <i>Pseudomonas aeruginosa</i>, and <i>Klebsiella pneumoniae</i>, as well as mixed-species biofilms. Our results propose that the application of SiL-gel affects different components of biofilm extracellular polymeric substances, disrupts the matrix, and eliminates the bacteria embedded in it. This composition is highly effective against biofilms composed of Gram-negative and Gram-positive species and does not possess significant cytotoxic effects. Our data form the basis for the development of antibacterial skin care products with a gentle but effective mode of action. |
| first_indexed | 2024-03-08T10:54:08Z |
| format | Article |
| id | doaj.art-459f339ea7fb45d3bf2b8c418402e3c0 |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-08T10:54:08Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj.art-459f339ea7fb45d3bf2b8c418402e3c02024-01-26T16:40:14ZengMDPI AGGels2310-28612024-01-011016010.3390/gels10010060Alginate Gel Encapsulated with Enzybiotics Cocktail Is Effective against Multispecies BiofilmsDaria V. Vasina0Nataliia P. Antonova1Elena V. Shidlovskaya2Nadezhda A. Kuznetsova3Alexander V. Grishin4Elizaveta A. Akoulina5Ekaterina A. Trusova6Anastasiya M. Lendel7Elena P. Mazunina8Sofia R. Kozlova9Andrei A. Dudun10Anton P. Bonartsev11Vladimir G. Lunin12Vladimir A. Gushchin13N.F. Gamaleya National Research Centre for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, 123098 Moscow, RussiaN.F. Gamaleya National Research Centre for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, 123098 Moscow, RussiaN.F. Gamaleya National Research Centre for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, 123098 Moscow, RussiaN.F. Gamaleya National Research Centre for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, 123098 Moscow, RussiaN.F. Gamaleya National Research Centre for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, 123098 Moscow, RussiaFaculty of Biology, MSU-BIT Shenzhen University, Shenzhen 518115, ChinaFaculty of Biology, MSU-BIT Shenzhen University, Shenzhen 518115, ChinaN.F. Gamaleya National Research Centre for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, 123098 Moscow, RussiaN.F. Gamaleya National Research Centre for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, 123098 Moscow, RussiaN.F. Gamaleya National Research Centre for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, 123098 Moscow, RussiaResearch Center of Biotechnology of the Russian Academy of Sciences Leninsky Ave, 33, Bld. 2, 119071 Moscow, RussiaFaculty of Biology, M.V. Lomonosov Moscow State University, 119234 Moscow, RussiaN.F. Gamaleya National Research Centre for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, 123098 Moscow, RussiaN.F. Gamaleya National Research Centre for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, 123098 Moscow, RussiaThe development of new and effective antibacterials for pharmaceutical or cosmetic skin care that have a low potential for the emergence and expansion of bacterial resistance is of high demand in scientific and applied research. Great hopes are placed on alternative agents such as bactericidal peptidoglycan hydrolases, depolymerases, etc. Enzybiotic-based preparations are being studied for the treatment of various infections and, among others, can be used as topical formulations and dressings with protein-polysaccharide complexes. Here, we investigate the antibiofilm properties of a novel enzybiotic cocktail of phage endolysin LysSi3 and bacteriocin lysostaphin, formulated in the alginate gel matrix and its ability to control the opportunistic skin-colonizing bacteria <i>Staphylococcus aureus</i>, <i>Pseudomonas aeruginosa</i>, and <i>Klebsiella pneumoniae</i>, as well as mixed-species biofilms. Our results propose that the application of SiL-gel affects different components of biofilm extracellular polymeric substances, disrupts the matrix, and eliminates the bacteria embedded in it. This composition is highly effective against biofilms composed of Gram-negative and Gram-positive species and does not possess significant cytotoxic effects. Our data form the basis for the development of antibacterial skin care products with a gentle but effective mode of action.https://www.mdpi.com/2310-2861/10/1/60alginate gelenzybioticsendolysinlysostaphinbiofilmspolymicrobial infection |
| spellingShingle | Daria V. Vasina Nataliia P. Antonova Elena V. Shidlovskaya Nadezhda A. Kuznetsova Alexander V. Grishin Elizaveta A. Akoulina Ekaterina A. Trusova Anastasiya M. Lendel Elena P. Mazunina Sofia R. Kozlova Andrei A. Dudun Anton P. Bonartsev Vladimir G. Lunin Vladimir A. Gushchin Alginate Gel Encapsulated with Enzybiotics Cocktail Is Effective against Multispecies Biofilms Gels alginate gel enzybiotics endolysin lysostaphin biofilms polymicrobial infection |
| title | Alginate Gel Encapsulated with Enzybiotics Cocktail Is Effective against Multispecies Biofilms |
| title_full | Alginate Gel Encapsulated with Enzybiotics Cocktail Is Effective against Multispecies Biofilms |
| title_fullStr | Alginate Gel Encapsulated with Enzybiotics Cocktail Is Effective against Multispecies Biofilms |
| title_full_unstemmed | Alginate Gel Encapsulated with Enzybiotics Cocktail Is Effective against Multispecies Biofilms |
| title_short | Alginate Gel Encapsulated with Enzybiotics Cocktail Is Effective against Multispecies Biofilms |
| title_sort | alginate gel encapsulated with enzybiotics cocktail is effective against multispecies biofilms |
| topic | alginate gel enzybiotics endolysin lysostaphin biofilms polymicrobial infection |
| url | https://www.mdpi.com/2310-2861/10/1/60 |
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