Hexafluoropropylene oxide tetramer acid (HFPO-TeA)-induced developmental toxicities in chicken embryo: Peroxisome proliferator-activated receptor Alpha (PPARα) is involved
Hexafluoropropylene oxide tetramer acid (HFPO-TeA) is an emerging environmental contaminant, with environmental presence but limited toxicological information. To investigate its potential developmental toxicities, various doses of HFPO-TeA exposure were achieved in chicken embryos via air cell inje...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-03-01
|
| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651323001756 |
| _version_ | 1827989466304741376 |
|---|---|
| author | Qixuan Dong Yajie Guo Junhua Yuan Shuping Zhong Hao Ni Jingyi Liu Mengzhen Zhang Jiaqi Sun Shuqi Yuan Huan Yu Yuxu Zhong Qixiao Jiang |
| author_facet | Qixuan Dong Yajie Guo Junhua Yuan Shuping Zhong Hao Ni Jingyi Liu Mengzhen Zhang Jiaqi Sun Shuqi Yuan Huan Yu Yuxu Zhong Qixiao Jiang |
| author_sort | Qixuan Dong |
| collection | DOAJ |
| description | Hexafluoropropylene oxide tetramer acid (HFPO-TeA) is an emerging environmental contaminant, with environmental presence but limited toxicological information. To investigate its potential developmental toxicities, various doses of HFPO-TeA exposure were achieved in chicken embryos via air cell injection, and the exposed embryos were incubated until hatch. Within 24 h of hatch, the hatchling chickens were assessed with electrocardiography and histopathology for toxicological evaluation. For mechanistic investigation, in ovo silencing of PPARα was achieved via lentivirus microinjection, then the morphological/functional endpoints along with protein expression levels of PPARα-regulated genes were assessed. HFPO-TeA exposure in chicken embryo resulted in developmental cardiotoxicity and hepatotoxicity. Specifically, decreased right ventricular wall thickness, increased heart rate and hepatic steatosis were observed, whereas silencing of PPARα resulted in alleviation of observed toxicities. Western blotting for EHHADH and FABPs suggested that developmental exposure to HFPO-TeA effectively increased the expression levels of both targets in hatchling chicken heart and liver tissue samples, while PPARα silencing prevented such changes, suggesting that PPARα and its downstream genes are playing critical roles in HFPO-TeA induced developmental toxicities. |
| first_indexed | 2024-04-10T00:19:47Z |
| format | Article |
| id | doaj.art-45a03e18e37d418b83285b5f42b079a2 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-10T00:19:47Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-45a03e18e37d418b83285b5f42b079a22023-03-16T05:01:18ZengElsevierEcotoxicology and Environmental Safety0147-65132023-03-01253114671Hexafluoropropylene oxide tetramer acid (HFPO-TeA)-induced developmental toxicities in chicken embryo: Peroxisome proliferator-activated receptor Alpha (PPARα) is involvedQixuan Dong0Yajie Guo1Junhua Yuan2Shuping Zhong3Hao Ni4Jingyi Liu5Mengzhen Zhang6Jiaqi Sun7Shuqi Yuan8Huan Yu9Yuxu Zhong10Qixiao Jiang11Department of Toxicology, School of Public Health, Qingdao University, 308 Ningxia Road, Qingdao, ChinaDepartment of Toxicology, School of Public Health, Qingdao University, 308 Ningxia Road, Qingdao, ChinaDepartment of Special Medicine, School of Basic Medicine, Qingdao University, 308 Ningxia Road, Qingdao, ChinaDepartment of Toxicology, School of Public Health, Qingdao University, 308 Ningxia Road, Qingdao, ChinaDepartment of Toxicology, School of Public Health, Qingdao University, 308 Ningxia Road, Qingdao, ChinaDepartment of Toxicology, School of Public Health, Qingdao University, 308 Ningxia Road, Qingdao, ChinaDepartment of Toxicology, School of Public Health, Qingdao University, 308 Ningxia Road, Qingdao, ChinaDepartment of Toxicology, School of Public Health, Qingdao University, 308 Ningxia Road, Qingdao, ChinaDepartment of Toxicology, School of Public Health, Qingdao University, 308 Ningxia Road, Qingdao, ChinaDepartment of Toxicology, School of Public Health, Qingdao University, 308 Ningxia Road, Qingdao, ChinaState Key Laboratory of Toxicology and Medical Countermeasures, Beijing Institute of Pharmacology and Toxicology, 27 Taiping Road, Beijing, China; Correspondence to: State Key Laboratory of Toxicology and Medical Countermeasures, Beijing Institute of Pharmacology and Toxicology, Beijing, China.Department of Toxicology, School of Public Health, Qingdao University, 308 Ningxia Road, Qingdao, China; Correspondence to: Department of Toxicology, School of Public Health, Qingdao University, Qingdao, China.Hexafluoropropylene oxide tetramer acid (HFPO-TeA) is an emerging environmental contaminant, with environmental presence but limited toxicological information. To investigate its potential developmental toxicities, various doses of HFPO-TeA exposure were achieved in chicken embryos via air cell injection, and the exposed embryos were incubated until hatch. Within 24 h of hatch, the hatchling chickens were assessed with electrocardiography and histopathology for toxicological evaluation. For mechanistic investigation, in ovo silencing of PPARα was achieved via lentivirus microinjection, then the morphological/functional endpoints along with protein expression levels of PPARα-regulated genes were assessed. HFPO-TeA exposure in chicken embryo resulted in developmental cardiotoxicity and hepatotoxicity. Specifically, decreased right ventricular wall thickness, increased heart rate and hepatic steatosis were observed, whereas silencing of PPARα resulted in alleviation of observed toxicities. Western blotting for EHHADH and FABPs suggested that developmental exposure to HFPO-TeA effectively increased the expression levels of both targets in hatchling chicken heart and liver tissue samples, while PPARα silencing prevented such changes, suggesting that PPARα and its downstream genes are playing critical roles in HFPO-TeA induced developmental toxicities.http://www.sciencedirect.com/science/article/pii/S0147651323001756Hexafluoropropylene oxide tetramer acidPeroxisome proliferator-activated receptor AlphaDevelopmental toxicitiesChicken embryoIn ovo silencing |
| spellingShingle | Qixuan Dong Yajie Guo Junhua Yuan Shuping Zhong Hao Ni Jingyi Liu Mengzhen Zhang Jiaqi Sun Shuqi Yuan Huan Yu Yuxu Zhong Qixiao Jiang Hexafluoropropylene oxide tetramer acid (HFPO-TeA)-induced developmental toxicities in chicken embryo: Peroxisome proliferator-activated receptor Alpha (PPARα) is involved Ecotoxicology and Environmental Safety Hexafluoropropylene oxide tetramer acid Peroxisome proliferator-activated receptor Alpha Developmental toxicities Chicken embryo In ovo silencing |
| title | Hexafluoropropylene oxide tetramer acid (HFPO-TeA)-induced developmental toxicities in chicken embryo: Peroxisome proliferator-activated receptor Alpha (PPARα) is involved |
| title_full | Hexafluoropropylene oxide tetramer acid (HFPO-TeA)-induced developmental toxicities in chicken embryo: Peroxisome proliferator-activated receptor Alpha (PPARα) is involved |
| title_fullStr | Hexafluoropropylene oxide tetramer acid (HFPO-TeA)-induced developmental toxicities in chicken embryo: Peroxisome proliferator-activated receptor Alpha (PPARα) is involved |
| title_full_unstemmed | Hexafluoropropylene oxide tetramer acid (HFPO-TeA)-induced developmental toxicities in chicken embryo: Peroxisome proliferator-activated receptor Alpha (PPARα) is involved |
| title_short | Hexafluoropropylene oxide tetramer acid (HFPO-TeA)-induced developmental toxicities in chicken embryo: Peroxisome proliferator-activated receptor Alpha (PPARα) is involved |
| title_sort | hexafluoropropylene oxide tetramer acid hfpo tea induced developmental toxicities in chicken embryo peroxisome proliferator activated receptor alpha pparα is involved |
| topic | Hexafluoropropylene oxide tetramer acid Peroxisome proliferator-activated receptor Alpha Developmental toxicities Chicken embryo In ovo silencing |
| url | http://www.sciencedirect.com/science/article/pii/S0147651323001756 |
| work_keys_str_mv | AT qixuandong hexafluoropropyleneoxidetetrameracidhfpoteainduceddevelopmentaltoxicitiesinchickenembryoperoxisomeproliferatoractivatedreceptoralphapparaisinvolved AT yajieguo hexafluoropropyleneoxidetetrameracidhfpoteainduceddevelopmentaltoxicitiesinchickenembryoperoxisomeproliferatoractivatedreceptoralphapparaisinvolved AT junhuayuan hexafluoropropyleneoxidetetrameracidhfpoteainduceddevelopmentaltoxicitiesinchickenembryoperoxisomeproliferatoractivatedreceptoralphapparaisinvolved AT shupingzhong hexafluoropropyleneoxidetetrameracidhfpoteainduceddevelopmentaltoxicitiesinchickenembryoperoxisomeproliferatoractivatedreceptoralphapparaisinvolved AT haoni hexafluoropropyleneoxidetetrameracidhfpoteainduceddevelopmentaltoxicitiesinchickenembryoperoxisomeproliferatoractivatedreceptoralphapparaisinvolved AT jingyiliu hexafluoropropyleneoxidetetrameracidhfpoteainduceddevelopmentaltoxicitiesinchickenembryoperoxisomeproliferatoractivatedreceptoralphapparaisinvolved AT mengzhenzhang hexafluoropropyleneoxidetetrameracidhfpoteainduceddevelopmentaltoxicitiesinchickenembryoperoxisomeproliferatoractivatedreceptoralphapparaisinvolved AT jiaqisun hexafluoropropyleneoxidetetrameracidhfpoteainduceddevelopmentaltoxicitiesinchickenembryoperoxisomeproliferatoractivatedreceptoralphapparaisinvolved AT shuqiyuan hexafluoropropyleneoxidetetrameracidhfpoteainduceddevelopmentaltoxicitiesinchickenembryoperoxisomeproliferatoractivatedreceptoralphapparaisinvolved AT huanyu hexafluoropropyleneoxidetetrameracidhfpoteainduceddevelopmentaltoxicitiesinchickenembryoperoxisomeproliferatoractivatedreceptoralphapparaisinvolved AT yuxuzhong hexafluoropropyleneoxidetetrameracidhfpoteainduceddevelopmentaltoxicitiesinchickenembryoperoxisomeproliferatoractivatedreceptoralphapparaisinvolved AT qixiaojiang hexafluoropropyleneoxidetetrameracidhfpoteainduceddevelopmentaltoxicitiesinchickenembryoperoxisomeproliferatoractivatedreceptoralphapparaisinvolved |