Multi-Modal Multi-Spectral Intravital Macroscopic Imaging of Signaling Dynamics in Real Time during Tumor–Immune Interactions
A major obstacle in studying the interplay between cancer cells and the immune system has been the examination of proposed biological pathways and cell interactions in a dynamic, physiologically relevant system in vivo. Intravital imaging strategies are one of the few molecular imaging techniques th...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-02-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/10/3/489 |
| _version_ | 1827588361747955712 |
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| author | Tracy W. Liu Seth T. Gammon David Fuentes David Piwnica-Worms |
| author_facet | Tracy W. Liu Seth T. Gammon David Fuentes David Piwnica-Worms |
| author_sort | Tracy W. Liu |
| collection | DOAJ |
| description | A major obstacle in studying the interplay between cancer cells and the immune system has been the examination of proposed biological pathways and cell interactions in a dynamic, physiologically relevant system in vivo. Intravital imaging strategies are one of the few molecular imaging techniques that can follow biological processes at cellular resolution over long periods of time in the same individual. Bioluminescence imaging has become a standard preclinical in vivo optical imaging technique with ever-expanding versatility as a result of the development of new emission bioluminescent reporters, advances in genomic techniques, and technical improvements in bioluminescence imaging and processing methods. Herein, we describe an advance of technology with a molecular imaging window chamber platform that combines bioluminescent and fluorescent reporters with intravital macro-imaging techniques and bioluminescence spectral unmixing in real time applied to heterogeneous living systems in vivo for evaluating tumor signaling dynamics and immune cell enzyme activities concurrently. |
| first_indexed | 2024-03-09T00:33:20Z |
| format | Article |
| id | doaj.art-45a4120c3c4f497c9256563b60b7e8e4 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T00:33:20Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-45a4120c3c4f497c9256563b60b7e8e42023-12-11T18:19:08ZengMDPI AGCells2073-44092021-02-0110348910.3390/cells10030489Multi-Modal Multi-Spectral Intravital Macroscopic Imaging of Signaling Dynamics in Real Time during Tumor–Immune InteractionsTracy W. Liu0Seth T. Gammon1David Fuentes2David Piwnica-Worms3Department of Cancer Systems Imaging, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USADepartment of Cancer Systems Imaging, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USADepartment of Imaging Physics, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USADepartment of Cancer Systems Imaging, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USAA major obstacle in studying the interplay between cancer cells and the immune system has been the examination of proposed biological pathways and cell interactions in a dynamic, physiologically relevant system in vivo. Intravital imaging strategies are one of the few molecular imaging techniques that can follow biological processes at cellular resolution over long periods of time in the same individual. Bioluminescence imaging has become a standard preclinical in vivo optical imaging technique with ever-expanding versatility as a result of the development of new emission bioluminescent reporters, advances in genomic techniques, and technical improvements in bioluminescence imaging and processing methods. Herein, we describe an advance of technology with a molecular imaging window chamber platform that combines bioluminescent and fluorescent reporters with intravital macro-imaging techniques and bioluminescence spectral unmixing in real time applied to heterogeneous living systems in vivo for evaluating tumor signaling dynamics and immune cell enzyme activities concurrently.https://www.mdpi.com/2073-4409/10/3/489intravital imagingbioluminescenceimmune cell imagingimaging reportersmolecular imagingtumor signaling |
| spellingShingle | Tracy W. Liu Seth T. Gammon David Fuentes David Piwnica-Worms Multi-Modal Multi-Spectral Intravital Macroscopic Imaging of Signaling Dynamics in Real Time during Tumor–Immune Interactions Cells intravital imaging bioluminescence immune cell imaging imaging reporters molecular imaging tumor signaling |
| title | Multi-Modal Multi-Spectral Intravital Macroscopic Imaging of Signaling Dynamics in Real Time during Tumor–Immune Interactions |
| title_full | Multi-Modal Multi-Spectral Intravital Macroscopic Imaging of Signaling Dynamics in Real Time during Tumor–Immune Interactions |
| title_fullStr | Multi-Modal Multi-Spectral Intravital Macroscopic Imaging of Signaling Dynamics in Real Time during Tumor–Immune Interactions |
| title_full_unstemmed | Multi-Modal Multi-Spectral Intravital Macroscopic Imaging of Signaling Dynamics in Real Time during Tumor–Immune Interactions |
| title_short | Multi-Modal Multi-Spectral Intravital Macroscopic Imaging of Signaling Dynamics in Real Time during Tumor–Immune Interactions |
| title_sort | multi modal multi spectral intravital macroscopic imaging of signaling dynamics in real time during tumor immune interactions |
| topic | intravital imaging bioluminescence immune cell imaging imaging reporters molecular imaging tumor signaling |
| url | https://www.mdpi.com/2073-4409/10/3/489 |
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