Live Vaccinia Virus-Coated Microneedle Array Patches for Smallpox Vaccination and Stockpiling
Although smallpox has been eradicated globally, the potential use of the smallpox virus in bioterrorism indicates the importance of stockpiling smallpox vaccines. Considering the advantages of microneedle-based vaccination over conventional needle injections, in this study, we examined the feasibili...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-02-01
|
| Series: | Pharmaceutics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4923/13/2/209 |
| _version_ | 1797415755369676800 |
|---|---|
| author | In-Jeong Choi Hye-Ran Cha Su Jin Hwang Seung-Ki Baek Jae Myun Lee Seong-O Choi |
| author_facet | In-Jeong Choi Hye-Ran Cha Su Jin Hwang Seung-Ki Baek Jae Myun Lee Seong-O Choi |
| author_sort | In-Jeong Choi |
| collection | DOAJ |
| description | Although smallpox has been eradicated globally, the potential use of the smallpox virus in bioterrorism indicates the importance of stockpiling smallpox vaccines. Considering the advantages of microneedle-based vaccination over conventional needle injections, in this study, we examined the feasibility of microneedle-based smallpox vaccination as an alternative approach for stockpiling smallpox vaccines. We prepared polylactic acid (PLA) microneedle array patches by micromolding and loaded a second-generation smallpox vaccine on the microneedle tips via dip coating. We evaluated the effect of excipients and drying conditions on vaccine stability in vitro and examined immune responses in female BALB/c mice by measuring neutralizing antibodies and interferon (IFN)-γ-secreting cells. Approximately 40% of the virus titer was reduced during the vaccine-coating process, with or without excipients. At −20 °C, the smallpox vaccine coated on the microneedles was stable up to 6 months. Compared to natural evaporation, vacuum drying was more efficient in improving the smallpox vaccine stability. Microneedle-based vaccination of the mice elicited neutralizing antibodies beginning 3 weeks after immunization; the levels were maintained for 12 weeks. It significantly increased IFN-γ-secreting cells 12 weeks after priming, indicating the induction of cellular immune responses. The smallpox-vaccine-coated microneedles could serve as an alternative delivery system for vaccination and stockpiling. |
| first_indexed | 2024-03-09T05:54:09Z |
| format | Article |
| id | doaj.art-45a4911a08ff40f0a02665c67729b908 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T05:54:09Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-45a4911a08ff40f0a02665c67729b9082023-12-03T12:15:06ZengMDPI AGPharmaceutics1999-49232021-02-0113220910.3390/pharmaceutics13020209Live Vaccinia Virus-Coated Microneedle Array Patches for Smallpox Vaccination and StockpilingIn-Jeong Choi0Hye-Ran Cha1Su Jin Hwang2Seung-Ki Baek3Jae Myun Lee4Seong-O Choi5QuadMedicine R&D Centre, QuadMedicine, Inc., Seongnam 13209, KoreaDepartment of Microbiology and Immunology, Institute for Immunology and Immunological Diseases, Graduate School of Medical Science, Brain Korea 21 Project, Yonsei University College of Medicine, Seoul 03722, KoreaDepartment of Microbiology and Immunology, Institute for Immunology and Immunological Diseases, Graduate School of Medical Science, Brain Korea 21 Project, Yonsei University College of Medicine, Seoul 03722, KoreaQuadMedicine R&D Centre, QuadMedicine, Inc., Seongnam 13209, KoreaDepartment of Microbiology and Immunology, Institute for Immunology and Immunological Diseases, Graduate School of Medical Science, Brain Korea 21 Project, Yonsei University College of Medicine, Seoul 03722, KoreaQuadMedicine R&D Centre, QuadMedicine, Inc., Seongnam 13209, KoreaAlthough smallpox has been eradicated globally, the potential use of the smallpox virus in bioterrorism indicates the importance of stockpiling smallpox vaccines. Considering the advantages of microneedle-based vaccination over conventional needle injections, in this study, we examined the feasibility of microneedle-based smallpox vaccination as an alternative approach for stockpiling smallpox vaccines. We prepared polylactic acid (PLA) microneedle array patches by micromolding and loaded a second-generation smallpox vaccine on the microneedle tips via dip coating. We evaluated the effect of excipients and drying conditions on vaccine stability in vitro and examined immune responses in female BALB/c mice by measuring neutralizing antibodies and interferon (IFN)-γ-secreting cells. Approximately 40% of the virus titer was reduced during the vaccine-coating process, with or without excipients. At −20 °C, the smallpox vaccine coated on the microneedles was stable up to 6 months. Compared to natural evaporation, vacuum drying was more efficient in improving the smallpox vaccine stability. Microneedle-based vaccination of the mice elicited neutralizing antibodies beginning 3 weeks after immunization; the levels were maintained for 12 weeks. It significantly increased IFN-γ-secreting cells 12 weeks after priming, indicating the induction of cellular immune responses. The smallpox-vaccine-coated microneedles could serve as an alternative delivery system for vaccination and stockpiling.https://www.mdpi.com/1999-4923/13/2/209smallpoxmicroneedlevaccinia viruscoatingstabilityimmune response |
| spellingShingle | In-Jeong Choi Hye-Ran Cha Su Jin Hwang Seung-Ki Baek Jae Myun Lee Seong-O Choi Live Vaccinia Virus-Coated Microneedle Array Patches for Smallpox Vaccination and Stockpiling Pharmaceutics smallpox microneedle vaccinia virus coating stability immune response |
| title | Live Vaccinia Virus-Coated Microneedle Array Patches for Smallpox Vaccination and Stockpiling |
| title_full | Live Vaccinia Virus-Coated Microneedle Array Patches for Smallpox Vaccination and Stockpiling |
| title_fullStr | Live Vaccinia Virus-Coated Microneedle Array Patches for Smallpox Vaccination and Stockpiling |
| title_full_unstemmed | Live Vaccinia Virus-Coated Microneedle Array Patches for Smallpox Vaccination and Stockpiling |
| title_short | Live Vaccinia Virus-Coated Microneedle Array Patches for Smallpox Vaccination and Stockpiling |
| title_sort | live vaccinia virus coated microneedle array patches for smallpox vaccination and stockpiling |
| topic | smallpox microneedle vaccinia virus coating stability immune response |
| url | https://www.mdpi.com/1999-4923/13/2/209 |
| work_keys_str_mv | AT injeongchoi livevacciniaviruscoatedmicroneedlearraypatchesforsmallpoxvaccinationandstockpiling AT hyerancha livevacciniaviruscoatedmicroneedlearraypatchesforsmallpoxvaccinationandstockpiling AT sujinhwang livevacciniaviruscoatedmicroneedlearraypatchesforsmallpoxvaccinationandstockpiling AT seungkibaek livevacciniaviruscoatedmicroneedlearraypatchesforsmallpoxvaccinationandstockpiling AT jaemyunlee livevacciniaviruscoatedmicroneedlearraypatchesforsmallpoxvaccinationandstockpiling AT seongochoi livevacciniaviruscoatedmicroneedlearraypatchesforsmallpoxvaccinationandstockpiling |