Extracellular Biosynthesis of Silver Nanoparticles Using Fungi and Their Antibacterial Activity
Silver nanoparticles have particular properties that contribute to their very promising applications, novel in various fields of science, such as the development of biosensors, the diagnosis and treatment of cancer, the controlled release of drugs and the antimicrobial potential. The biological synt...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2018-06-01
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| Series: | Nano Biomedicine and Engineering |
| Subjects: | |
| Online Access: | https://www.sciopen.com/article/10.5101/nbe.v10i2.p165-173 |
| _version_ | 1797673367653842944 |
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| author | Paula Sanguiñedo Raluca María Fratila María Belén Estevez Jesús Martínez de la Fuente Valeria Grazú Silvana Alborés |
| author_facet | Paula Sanguiñedo Raluca María Fratila María Belén Estevez Jesús Martínez de la Fuente Valeria Grazú Silvana Alborés |
| author_sort | Paula Sanguiñedo |
| collection | DOAJ |
| description | Silver nanoparticles have particular properties that contribute to their very promising applications, novel in various fields of science, such as the development of biosensors, the diagnosis and treatment of cancer, the controlled release of drugs and the antimicrobial potential. The biological synthesis of nanoparticles is of great interest over other physical and chemical methods because the use of toxic chemicals and drastic reaction conditions are avoided. The extracellular biosynthesis using fungi could also make downstream processing much easier than the intracellular biosynthesis. One of the main applications of silver nanoparticles is their antimicrobial activity. Several studies have demonstrated the bactericidal properties of silver nanoparticles are different from silver ions, and that they are strongly influenced by their shape, size, concentration and colloidal state. In the present work, the ability of fungal strains from Uruguay to synthesize silver nanoparticles was studied. Eight fungi were able to synthesize nanoparticles. An extensive physicochemical characterization of the nanoparticles was carried out including ultraviolet-visible spectroscopy, transmission electron microscopy, dynamic light scattering, zeta-potential and gel electrophoretic mobility. According to the characterization and colloidal stability results, nanoparticles from three fungi were selected for antimicrobial activity assays. All nanoparticles were able to inhibit Escherichia coli growth, demonstrating their potential as effective antibacterial agent for use in biomedical applications. |
| first_indexed | 2024-03-11T21:43:29Z |
| format | Article |
| id | doaj.art-45aae41299364baeacdeca17769e47f1 |
| institution | Directory Open Access Journal |
| issn | 2150-5578 |
| language | English |
| last_indexed | 2024-03-11T21:43:29Z |
| publishDate | 2018-06-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Nano Biomedicine and Engineering |
| spelling | doaj.art-45aae41299364baeacdeca17769e47f12023-09-26T11:02:24ZengTsinghua University PressNano Biomedicine and Engineering2150-55782018-06-0110216517310.5101/nbe.v10i2.p165-173Extracellular Biosynthesis of Silver Nanoparticles Using Fungi and Their Antibacterial ActivityPaula Sanguiñedo0Raluca María Fratila1María Belén Estevez2Jesús Martínez de la Fuente3Valeria Grazú4Silvana Alborés5Área de Microbiología, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, UruguayInstituto de Ciencia de Materiales de Aragón, CSIC, Zaragoza, EspañaÁrea de Microbiología, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, UruguayInstituto de Ciencia de Materiales de Aragón, CSIC, Zaragoza, EspañaInstituto de Ciencia de Materiales de Aragón, CSIC, Zaragoza, EspañaÁrea de Microbiología, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, UruguaySilver nanoparticles have particular properties that contribute to their very promising applications, novel in various fields of science, such as the development of biosensors, the diagnosis and treatment of cancer, the controlled release of drugs and the antimicrobial potential. The biological synthesis of nanoparticles is of great interest over other physical and chemical methods because the use of toxic chemicals and drastic reaction conditions are avoided. The extracellular biosynthesis using fungi could also make downstream processing much easier than the intracellular biosynthesis. One of the main applications of silver nanoparticles is their antimicrobial activity. Several studies have demonstrated the bactericidal properties of silver nanoparticles are different from silver ions, and that they are strongly influenced by their shape, size, concentration and colloidal state. In the present work, the ability of fungal strains from Uruguay to synthesize silver nanoparticles was studied. Eight fungi were able to synthesize nanoparticles. An extensive physicochemical characterization of the nanoparticles was carried out including ultraviolet-visible spectroscopy, transmission electron microscopy, dynamic light scattering, zeta-potential and gel electrophoretic mobility. According to the characterization and colloidal stability results, nanoparticles from three fungi were selected for antimicrobial activity assays. All nanoparticles were able to inhibit Escherichia coli growth, demonstrating their potential as effective antibacterial agent for use in biomedical applications.https://www.sciopen.com/article/10.5101/nbe.v10i2.p165-173silver nanoparticlesfungibiosynthesisantibacterial activity |
| spellingShingle | Paula Sanguiñedo Raluca María Fratila María Belén Estevez Jesús Martínez de la Fuente Valeria Grazú Silvana Alborés Extracellular Biosynthesis of Silver Nanoparticles Using Fungi and Their Antibacterial Activity Nano Biomedicine and Engineering silver nanoparticles fungi biosynthesis antibacterial activity |
| title | Extracellular Biosynthesis of Silver Nanoparticles Using Fungi and Their Antibacterial Activity |
| title_full | Extracellular Biosynthesis of Silver Nanoparticles Using Fungi and Their Antibacterial Activity |
| title_fullStr | Extracellular Biosynthesis of Silver Nanoparticles Using Fungi and Their Antibacterial Activity |
| title_full_unstemmed | Extracellular Biosynthesis of Silver Nanoparticles Using Fungi and Their Antibacterial Activity |
| title_short | Extracellular Biosynthesis of Silver Nanoparticles Using Fungi and Their Antibacterial Activity |
| title_sort | extracellular biosynthesis of silver nanoparticles using fungi and their antibacterial activity |
| topic | silver nanoparticles fungi biosynthesis antibacterial activity |
| url | https://www.sciopen.com/article/10.5101/nbe.v10i2.p165-173 |
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