Curcumin-Encapsulated Nanomicelles Improve Cellular Uptake and Cytotoxicity in Cisplatin-Resistant Human Oral Cancer Cells
Oral cancer has a high mortality rate, which is mostly determined by the stage of the disease at the time of admission. Around half of all patients with oral cancer report with advanced illness. Hitherto, chemotherapy is preferred to treat oral cancer, but the emergence of resistance to anti-cancer...
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MDPI AG
2022-09-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/13/4/158 |
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| author | Vijay M. Kumbar Uday Muddapur Abdullatif Bin Muhsinah Saad Ali Alshehri Mohammed Merae Alshahrani Ibrahim Abdullah Almazni Manohar S. Kugaji Kishore Bhat Malleswara Rao Peram Mater H. Mahnashi Sameer J. Nadaf Sheetalnath B. Rooge Aejaz Abdullatif Khan Ibrahim Ahmed Shaikh |
| author_facet | Vijay M. Kumbar Uday Muddapur Abdullatif Bin Muhsinah Saad Ali Alshehri Mohammed Merae Alshahrani Ibrahim Abdullah Almazni Manohar S. Kugaji Kishore Bhat Malleswara Rao Peram Mater H. Mahnashi Sameer J. Nadaf Sheetalnath B. Rooge Aejaz Abdullatif Khan Ibrahim Ahmed Shaikh |
| author_sort | Vijay M. Kumbar |
| collection | DOAJ |
| description | Oral cancer has a high mortality rate, which is mostly determined by the stage of the disease at the time of admission. Around half of all patients with oral cancer report with advanced illness. Hitherto, chemotherapy is preferred to treat oral cancer, but the emergence of resistance to anti-cancer drugs is likely to occur after a sequence of treatments. Curcumin is renowned for its anticancer potential but its marred water solubility and poor bioavailability limit its use in treating multidrug-resistant cancers. As part of this investigation, we prepared and characterized Curcumin nanomicelles (CUR-NMs) using DSPE-PEG-2000 and evaluated the anticancer properties of cisplatin-resistant cancer cell lines. The prepared CUR-NMs were sphere-shaped and unilamellar in structure, with a size of 32.60 ± 4.2 nm. CUR-NMs exhibited high entrapment efficiency (82.2%), entrapment content (147.96 µg/mL), and a mean zeta potential of −17.5ζ which is considered moderately stable. The cellular uptake and cytotoxicity studies revealed that CUR-NMs had significantly higher cytotoxicity and cellular uptake in cisplatin drug-resistant oral cancer cell lines and parental oral cancer cells compared to plain curcumin (CUR). The DAPI and FACS analysis corroborated a high percentage of apoptotic cells with CUR-NMs (31.14%) compared to neat CUR (19.72%) treatment. Conclusively, CUR-NMs can potentially be used as an alternative carrier system to improve the therapeutic effects of curcumin in the treatment of cisplatin-resistant human oral cancer. |
| first_indexed | 2024-03-09T16:15:42Z |
| format | Article |
| id | doaj.art-45add6c6f23b4800ae0ae6c56e8c2e59 |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-09T16:15:42Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
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| series | Journal of Functional Biomaterials |
| spelling | doaj.art-45add6c6f23b4800ae0ae6c56e8c2e592023-11-24T15:48:37ZengMDPI AGJournal of Functional Biomaterials2079-49832022-09-0113415810.3390/jfb13040158Curcumin-Encapsulated Nanomicelles Improve Cellular Uptake and Cytotoxicity in Cisplatin-Resistant Human Oral Cancer CellsVijay M. Kumbar0Uday Muddapur1Abdullatif Bin Muhsinah2Saad Ali Alshehri3Mohammed Merae Alshahrani4Ibrahim Abdullah Almazni5Manohar S. Kugaji6Kishore Bhat7Malleswara Rao Peram8Mater H. Mahnashi9Sameer J. Nadaf10Sheetalnath B. Rooge11Aejaz Abdullatif Khan12Ibrahim Ahmed Shaikh13Central Research Laboratory, Maratha Mandal’s Nathajirao G. Halgekar Institute of Dental Sciences and Research Centre, Belagavi 590010, IndiaDepartment of Biotechnology, KLE Technological University, Hubballi 580031, IndiaDepartment of Pharmacognosy, College of Pharmacy, King Khalid University, Abha 61441, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, King Khalid University, Abha 61441, Saudi ArabiaDepartment of Clinical Laboratory Sciences, Faculty of Applied Medical Sciences, Najran University, Najran 61441, Saudi ArabiaDepartment of Clinical Laboratory Sciences, Faculty of Applied Medical Sciences, Najran University, Najran 61441, Saudi ArabiaCentral Research Laboratory, Maratha Mandal’s Nathajirao G. Halgekar Institute of Dental Sciences and Research Centre, Belagavi 590010, IndiaCentral Research Laboratory, Maratha Mandal’s Nathajirao G. Halgekar Institute of Dental Sciences and Research Centre, Belagavi 590010, IndiaChebrolu Hanumaiah Institute of Pharmaceutical Sciences, Guntur 522019, IndiaDepartment of Pharmaceutical Chemistry, College of Pharmacy, Najran University, Najran 66462, Saudi ArabiaSant Gajanan Maharaj College of Pharmacy, Mahagaon 416503, IndiaDepartment of Clinical Virology, Institute of Liver and Biliary Sciences, New Delhi 110070, IndiaDepartment of General Science, Ibn Sina National College for Medical Studies, Jeddah 21418, Saudi ArabiaDepartment of Pharmacology, College of Pharmacy, Najran University, Najran 66462, Saudi ArabiaOral cancer has a high mortality rate, which is mostly determined by the stage of the disease at the time of admission. Around half of all patients with oral cancer report with advanced illness. Hitherto, chemotherapy is preferred to treat oral cancer, but the emergence of resistance to anti-cancer drugs is likely to occur after a sequence of treatments. Curcumin is renowned for its anticancer potential but its marred water solubility and poor bioavailability limit its use in treating multidrug-resistant cancers. As part of this investigation, we prepared and characterized Curcumin nanomicelles (CUR-NMs) using DSPE-PEG-2000 and evaluated the anticancer properties of cisplatin-resistant cancer cell lines. The prepared CUR-NMs were sphere-shaped and unilamellar in structure, with a size of 32.60 ± 4.2 nm. CUR-NMs exhibited high entrapment efficiency (82.2%), entrapment content (147.96 µg/mL), and a mean zeta potential of −17.5ζ which is considered moderately stable. The cellular uptake and cytotoxicity studies revealed that CUR-NMs had significantly higher cytotoxicity and cellular uptake in cisplatin drug-resistant oral cancer cell lines and parental oral cancer cells compared to plain curcumin (CUR). The DAPI and FACS analysis corroborated a high percentage of apoptotic cells with CUR-NMs (31.14%) compared to neat CUR (19.72%) treatment. Conclusively, CUR-NMs can potentially be used as an alternative carrier system to improve the therapeutic effects of curcumin in the treatment of cisplatin-resistant human oral cancer.https://www.mdpi.com/2079-4983/13/4/158curcumin nanomicellesbioavailabilitypre-cancercisplatin-resistant oral cancercytotoxicity |
| spellingShingle | Vijay M. Kumbar Uday Muddapur Abdullatif Bin Muhsinah Saad Ali Alshehri Mohammed Merae Alshahrani Ibrahim Abdullah Almazni Manohar S. Kugaji Kishore Bhat Malleswara Rao Peram Mater H. Mahnashi Sameer J. Nadaf Sheetalnath B. Rooge Aejaz Abdullatif Khan Ibrahim Ahmed Shaikh Curcumin-Encapsulated Nanomicelles Improve Cellular Uptake and Cytotoxicity in Cisplatin-Resistant Human Oral Cancer Cells Journal of Functional Biomaterials curcumin nanomicelles bioavailability pre-cancer cisplatin-resistant oral cancer cytotoxicity |
| title | Curcumin-Encapsulated Nanomicelles Improve Cellular Uptake and Cytotoxicity in Cisplatin-Resistant Human Oral Cancer Cells |
| title_full | Curcumin-Encapsulated Nanomicelles Improve Cellular Uptake and Cytotoxicity in Cisplatin-Resistant Human Oral Cancer Cells |
| title_fullStr | Curcumin-Encapsulated Nanomicelles Improve Cellular Uptake and Cytotoxicity in Cisplatin-Resistant Human Oral Cancer Cells |
| title_full_unstemmed | Curcumin-Encapsulated Nanomicelles Improve Cellular Uptake and Cytotoxicity in Cisplatin-Resistant Human Oral Cancer Cells |
| title_short | Curcumin-Encapsulated Nanomicelles Improve Cellular Uptake and Cytotoxicity in Cisplatin-Resistant Human Oral Cancer Cells |
| title_sort | curcumin encapsulated nanomicelles improve cellular uptake and cytotoxicity in cisplatin resistant human oral cancer cells |
| topic | curcumin nanomicelles bioavailability pre-cancer cisplatin-resistant oral cancer cytotoxicity |
| url | https://www.mdpi.com/2079-4983/13/4/158 |
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