The role of phosphoinositide 3-kinases in neutrophil migration in 3D collagen gels.
The entry of neutrophils into tissue has been well characterised; however the fate of these cells once inside the tissue microenvironment is not fully understood. A variety of signal transduction pathways including those involving class I PI3 Kinases have been suggested to be involved in neutrophil...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4320071?pdf=render |
| _version_ | 1818246074813382656 |
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| author | Kayleigh J S Martin Michelle J Muessel Christine E Pullar Gary B Willars Andrew J Wardlaw |
| author_facet | Kayleigh J S Martin Michelle J Muessel Christine E Pullar Gary B Willars Andrew J Wardlaw |
| author_sort | Kayleigh J S Martin |
| collection | DOAJ |
| description | The entry of neutrophils into tissue has been well characterised; however the fate of these cells once inside the tissue microenvironment is not fully understood. A variety of signal transduction pathways including those involving class I PI3 Kinases have been suggested to be involved in neutrophil migration. This study aims to determine the involvement of PI3 Kinases in chemokinetic and chemotactic neutrophil migration in response to CXCL8 and GM-CSF in a three-dimensional collagen gel, as a model of tissue. Using a three-dimensional collagen assay chemokinetic and chemotactic migration induced by CXCL8 was inhibited with the pan PI3 Kinase inhibitor wortmannin. Analysis of the specific Class I PI3 Kinase catalytic isoforms alpha, delta and gamma using the inhibitors PIK-75, PIK-294 and AS-605240 respectively indicated differential roles in CXCL8-induced neutrophil migration. PIK-294 inhibited both chemokinetic and chemotactic CXCL8-induced migration. AS-605240 markedly reduced CXCL8 induced chemokinetic migration but had no effect on CXCL8 induced chemotactic migration. In contrast PIK-75 inhibited chemotactic migration but not chemokinetic migration. At optimal concentrations of GM-CSF the inhibitors had no effect on the percentage of neutrophil migration in comparison to the control however at suboptimal concentrations wortmannin, AS-605240 and PIK-294 inhibited chemokinesis. This study suggests that PI3 Kinase is necessary for CXCL8 induced migration in a 3D tissue environment but that chemokinetic and chemotactic migration may be controlled by different isoforms with gamma shown to be important in chemokinesis and alpha important in chemotaxis. Neutrophil migration in response to suboptimal concentrations of GM-CSF is dependent on PI3 Kinase, particularly the gamma and delta catalytic isoforms. |
| first_indexed | 2024-12-12T14:43:02Z |
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| id | doaj.art-45adf6b6b8e54557a36ce2adb04e2579 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T14:43:02Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-45adf6b6b8e54557a36ce2adb04e25792022-12-22T00:21:10ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01102e011625010.1371/journal.pone.0116250The role of phosphoinositide 3-kinases in neutrophil migration in 3D collagen gels.Kayleigh J S MartinMichelle J MuesselChristine E PullarGary B WillarsAndrew J WardlawThe entry of neutrophils into tissue has been well characterised; however the fate of these cells once inside the tissue microenvironment is not fully understood. A variety of signal transduction pathways including those involving class I PI3 Kinases have been suggested to be involved in neutrophil migration. This study aims to determine the involvement of PI3 Kinases in chemokinetic and chemotactic neutrophil migration in response to CXCL8 and GM-CSF in a three-dimensional collagen gel, as a model of tissue. Using a three-dimensional collagen assay chemokinetic and chemotactic migration induced by CXCL8 was inhibited with the pan PI3 Kinase inhibitor wortmannin. Analysis of the specific Class I PI3 Kinase catalytic isoforms alpha, delta and gamma using the inhibitors PIK-75, PIK-294 and AS-605240 respectively indicated differential roles in CXCL8-induced neutrophil migration. PIK-294 inhibited both chemokinetic and chemotactic CXCL8-induced migration. AS-605240 markedly reduced CXCL8 induced chemokinetic migration but had no effect on CXCL8 induced chemotactic migration. In contrast PIK-75 inhibited chemotactic migration but not chemokinetic migration. At optimal concentrations of GM-CSF the inhibitors had no effect on the percentage of neutrophil migration in comparison to the control however at suboptimal concentrations wortmannin, AS-605240 and PIK-294 inhibited chemokinesis. This study suggests that PI3 Kinase is necessary for CXCL8 induced migration in a 3D tissue environment but that chemokinetic and chemotactic migration may be controlled by different isoforms with gamma shown to be important in chemokinesis and alpha important in chemotaxis. Neutrophil migration in response to suboptimal concentrations of GM-CSF is dependent on PI3 Kinase, particularly the gamma and delta catalytic isoforms.http://europepmc.org/articles/PMC4320071?pdf=render |
| spellingShingle | Kayleigh J S Martin Michelle J Muessel Christine E Pullar Gary B Willars Andrew J Wardlaw The role of phosphoinositide 3-kinases in neutrophil migration in 3D collagen gels. PLoS ONE |
| title | The role of phosphoinositide 3-kinases in neutrophil migration in 3D collagen gels. |
| title_full | The role of phosphoinositide 3-kinases in neutrophil migration in 3D collagen gels. |
| title_fullStr | The role of phosphoinositide 3-kinases in neutrophil migration in 3D collagen gels. |
| title_full_unstemmed | The role of phosphoinositide 3-kinases in neutrophil migration in 3D collagen gels. |
| title_short | The role of phosphoinositide 3-kinases in neutrophil migration in 3D collagen gels. |
| title_sort | role of phosphoinositide 3 kinases in neutrophil migration in 3d collagen gels |
| url | http://europepmc.org/articles/PMC4320071?pdf=render |
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