Calculation of the Median Lethal Dose and Low Lethal Dose Using the CombiStats Biometric Software
The median lethal dose (LD50) and low lethal dose (LD10) are calculated in acute toxicity studies, as well as during specific activity assessment of some medicines. The aim of the study was to develop a procedure for using CombiStats to calculate LD50 and LD10. The authors proposed a step-by-step al...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | Russian |
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Federal State Budgetary Institution ‘Scientific Centre for Expert Evaluation of Medicinal Products’ of the Ministry of Health of the Russian Federation (FSBI ‘SCEEMP’)
2021-07-01
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| Series: | Регуляторные исследования и экспертиза лекарственных средств |
| Subjects: | |
| Online Access: | https://www.vedomostincesmp.ru/jour/article/view/344 |
| _version_ | 1826566696667709440 |
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| author | P. V. Shadrin T. A. Batuashvili L. V. Simutenko N. P. Neugodova |
| author_facet | P. V. Shadrin T. A. Batuashvili L. V. Simutenko N. P. Neugodova |
| author_sort | P. V. Shadrin |
| collection | DOAJ |
| description | The median lethal dose (LD50) and low lethal dose (LD10) are calculated in acute toxicity studies, as well as during specific activity assessment of some medicines. The aim of the study was to develop a procedure for using CombiStats to calculate LD50 and LD10. The authors proposed a step-by-step algorithm for processing bioassay results using the CombiStats biometric software (median effective dose determination model, probit analysis) with conversion of doses to simple fractions (fractions of the maximum dose) to calculate LD50 and LD10. They compared LD50 and LD10 calculation results obtained using CombiStats with those obtained using electronic spreadsheets according to the Bliss–Miller–Tainter–Prozorovsky method described in the State Pharmacopoeia of the Russian Federation (General Monograph 1.1.0014.15). It has been demonstrated that the use of CombiStats sometimes has advantages over the use of the pharmacoepoeial method. |
| first_indexed | 2024-03-07T19:16:59Z |
| format | Article |
| id | doaj.art-45ae5520b30d449bb6d15a3c39549b74 |
| institution | Directory Open Access Journal |
| issn | 3034-3062 3034-3453 |
| language | Russian |
| last_indexed | 2025-03-14T10:55:45Z |
| publishDate | 2021-07-01 |
| publisher | Federal State Budgetary Institution ‘Scientific Centre for Expert Evaluation of Medicinal Products’ of the Ministry of Health of the Russian Federation (FSBI ‘SCEEMP’) |
| record_format | Article |
| series | Регуляторные исследования и экспертиза лекарственных средств |
| spelling | doaj.art-45ae5520b30d449bb6d15a3c39549b742025-03-02T10:46:42ZrusFederal State Budgetary Institution ‘Scientific Centre for Expert Evaluation of Medicinal Products’ of the Ministry of Health of the Russian Federation (FSBI ‘SCEEMP’)Регуляторные исследования и экспертиза лекарственных средств3034-30623034-34532021-07-0111213514210.30895/1991-2919-2021-11-2-135-142265Calculation of the Median Lethal Dose and Low Lethal Dose Using the CombiStats Biometric SoftwareP. V. Shadrin0T. A. Batuashvili1L. V. Simutenko2N. P. Neugodova3Scientific Centre for Expert Evaluation of Medicinal ProductsScientific Centre for Expert Evaluation of Medicinal ProductsScientific Centre for Expert Evaluation of Medicinal ProductsScientific Centre for Expert Evaluation of Medicinal ProductsThe median lethal dose (LD50) and low lethal dose (LD10) are calculated in acute toxicity studies, as well as during specific activity assessment of some medicines. The aim of the study was to develop a procedure for using CombiStats to calculate LD50 and LD10. The authors proposed a step-by-step algorithm for processing bioassay results using the CombiStats biometric software (median effective dose determination model, probit analysis) with conversion of doses to simple fractions (fractions of the maximum dose) to calculate LD50 and LD10. They compared LD50 and LD10 calculation results obtained using CombiStats with those obtained using electronic spreadsheets according to the Bliss–Miller–Tainter–Prozorovsky method described in the State Pharmacopoeia of the Russian Federation (General Monograph 1.1.0014.15). It has been demonstrated that the use of CombiStats sometimes has advantages over the use of the pharmacoepoeial method.https://www.vedomostincesmp.ru/jour/article/view/344median lethal doseld<sub>50</sub>median effective doseed<sub>50</sub>low lethal doseld<sub>10</sub>combistatsbiometric softwaredose conversionmedicinal productssnake venom |
| spellingShingle | P. V. Shadrin T. A. Batuashvili L. V. Simutenko N. P. Neugodova Calculation of the Median Lethal Dose and Low Lethal Dose Using the CombiStats Biometric Software Регуляторные исследования и экспертиза лекарственных средств median lethal dose ld<sub>50</sub> median effective dose ed<sub>50</sub> low lethal dose ld<sub>10</sub> combistats biometric software dose conversion medicinal products snake venom |
| title | Calculation of the Median Lethal Dose and Low Lethal Dose Using the CombiStats Biometric Software |
| title_full | Calculation of the Median Lethal Dose and Low Lethal Dose Using the CombiStats Biometric Software |
| title_fullStr | Calculation of the Median Lethal Dose and Low Lethal Dose Using the CombiStats Biometric Software |
| title_full_unstemmed | Calculation of the Median Lethal Dose and Low Lethal Dose Using the CombiStats Biometric Software |
| title_short | Calculation of the Median Lethal Dose and Low Lethal Dose Using the CombiStats Biometric Software |
| title_sort | calculation of the median lethal dose and low lethal dose using the combistats biometric software |
| topic | median lethal dose ld<sub>50</sub> median effective dose ed<sub>50</sub> low lethal dose ld<sub>10</sub> combistats biometric software dose conversion medicinal products snake venom |
| url | https://www.vedomostincesmp.ru/jour/article/view/344 |
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