Evaluation of buffalo semen by Trypan blue/Giemsa staining and related fertility in vitro

The aim of this work was to verify the feasibility of an easy, quick double staining technique for evaluation of frozen-thawed semen to predict the fertilizing capability in vitro of buffalo bulls. In Experiment 1, frozen-thawed semen from 6 bulls was stained with double Trypan blue/ Giemsa and the...

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Bibliographic Details
Main Authors: B. Gasparrini, E. Mariotti, L. Attanasio, A. De Rosa, R. Di Palo, L. Boccia
Format: Article
Language:English
Published: Taylor & Francis Group 2010-02-01
Series:Italian Journal of Animal Science
Subjects:
Online Access:http://www.aspajournal.it/index.php/ijas/article/view/1095

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