| Summary: | Fungal infection of grape berries (<i>Vitis vinifera</i>) by <i>Botrytis cinerea</i> frequently coincides with harvest, impacting both the yield and quality of grape and wine products. A rapid and non-destructive method for identifying <i>B. cinerea</i> infection in grapes at an early stage prior to harvest is critical to manage loss. In this study, zeolitic imidazolate framework-8 (ZIF-8) crystal was applied as an absorbent material for volatile extraction from <i>B. cinerea</i> infected and healthy grapes in a vineyard, followed by thermal desorption gas chromatography–mass spectrometry. The performance of ZIF-8 in regard to absorbing and trapping the targeted volatiles was evaluated with a standard solution of compounds and with a whole bunch of grapes enclosed in a glass container to maintain standard sampling conditions. The results from the sampling methods were then correlated to <i>B. cinerea</i> infection in grapes, as measured and determined by genus-specific antigen quantification. Trace levels of targeted compounds reported as markers of grape <i>B. cinerea</i> infection were successfully detected with in-field sampling. The peak area counts for volatiles 3-octanone, 1-octen-3-one, 3-octanol, and 1-octen-3-ol extracted using ZIF-8 were significantly higher than values achieved using Tenax<sup>®</sup>-TA from field testing and demonstrated good correlation with <i>B. cinerea</i> infection severities determined by <i>B. cinerea</i> antigen detection.
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