Trans-grafting plum pox virus resistance from transgenic plum rootstocks to apricot scions
IntroductionTrans-grafting could be a strategy to transfer virus resistance from a transgenic rootstock to a wild type scion. However contradictory results have been obtained in herbaceous and woody plants. This work was intended to determine if the resistance to sharka could be transferred from tra...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1216217/full |
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| author | Nuria Alburquerque Cristian Pérez-Caselles Lydia Faize Vincenza Ilardi Lorenzo Burgos |
| author_facet | Nuria Alburquerque Cristian Pérez-Caselles Lydia Faize Vincenza Ilardi Lorenzo Burgos |
| author_sort | Nuria Alburquerque |
| collection | DOAJ |
| description | IntroductionTrans-grafting could be a strategy to transfer virus resistance from a transgenic rootstock to a wild type scion. However contradictory results have been obtained in herbaceous and woody plants. This work was intended to determine if the resistance to sharka could be transferred from transgenic plum rootstocks to wild-type apricot scions grafted onto them.MethodsTo this end, we conducted grafting experiments of wild- type apricots onto plum plants transformed with a construction codifying a hairpin RNA designed to silence the PPV virus and studied if the resistance was transmitted from the rootstock to the scion.ResultsOur data support that the RNA-silencing-based PPV resistance can be transmitted from PPV-resistant plum rootstocks to non-transgenic apricot scions and that its efficiency is augmented after successive growth cycles. PPV resistance conferred by the rootstocks was robust, already occurring within the same growing cycle and maintained in successive evaluation cycles. The RNA silencing mechanism reduces the relative accumulation of the virus progressively eliminating the virus from the wild type scions grafted on the transgenic resistant PPV plants. There was a preferential accumulation of the 24nt siRNAs in the scions grafted onto resistant rootstocks that was not found in the scions grafted on the susceptible rootstock. This matched with a significantly lower relative accumulation of hpRNA in the resistant rootstocks compared with the susceptible or the tolerant ones.DiscussionUsing transgenic rootstocks should mitigate public concerns about transgenes dispersion and eating transgenic food and allow conferring virus resistance to recalcitrant to transformation cultivars or species. |
| first_indexed | 2024-03-11T21:19:48Z |
| format | Article |
| id | doaj.art-45b3bfb184ce4a4d8f333393b43f3125 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-11T21:19:48Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-45b3bfb184ce4a4d8f333393b43f31252023-09-28T09:30:04ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-09-011410.3389/fpls.2023.12162171216217Trans-grafting plum pox virus resistance from transgenic plum rootstocks to apricot scionsNuria Alburquerque0Cristian Pérez-Caselles1Lydia Faize2Vincenza Ilardi3Lorenzo Burgos4Fruit Biotechnology Group, Department of Plant Breeding, Centro de Edafología y Biología Aplicada del Segura- Consejo Superior de Investigaciones Científicas (CEBAS-CSIC), Murcia, SpainFruit Biotechnology Group, Department of Plant Breeding, Centro de Edafología y Biología Aplicada del Segura- Consejo Superior de Investigaciones Científicas (CEBAS-CSIC), Murcia, SpainFruit Biotechnology Group, Department of Plant Breeding, Centro de Edafología y Biología Aplicada del Segura- Consejo Superior de Investigaciones Científicas (CEBAS-CSIC), Murcia, SpainResearch Centre for Plant Protection and Certification, Council for Agricultural Research and Economics (CREA-DC), Rome, ItalyFruit Biotechnology Group, Department of Plant Breeding, Centro de Edafología y Biología Aplicada del Segura- Consejo Superior de Investigaciones Científicas (CEBAS-CSIC), Murcia, SpainIntroductionTrans-grafting could be a strategy to transfer virus resistance from a transgenic rootstock to a wild type scion. However contradictory results have been obtained in herbaceous and woody plants. This work was intended to determine if the resistance to sharka could be transferred from transgenic plum rootstocks to wild-type apricot scions grafted onto them.MethodsTo this end, we conducted grafting experiments of wild- type apricots onto plum plants transformed with a construction codifying a hairpin RNA designed to silence the PPV virus and studied if the resistance was transmitted from the rootstock to the scion.ResultsOur data support that the RNA-silencing-based PPV resistance can be transmitted from PPV-resistant plum rootstocks to non-transgenic apricot scions and that its efficiency is augmented after successive growth cycles. PPV resistance conferred by the rootstocks was robust, already occurring within the same growing cycle and maintained in successive evaluation cycles. The RNA silencing mechanism reduces the relative accumulation of the virus progressively eliminating the virus from the wild type scions grafted on the transgenic resistant PPV plants. There was a preferential accumulation of the 24nt siRNAs in the scions grafted onto resistant rootstocks that was not found in the scions grafted on the susceptible rootstock. This matched with a significantly lower relative accumulation of hpRNA in the resistant rootstocks compared with the susceptible or the tolerant ones.DiscussionUsing transgenic rootstocks should mitigate public concerns about transgenes dispersion and eating transgenic food and allow conferring virus resistance to recalcitrant to transformation cultivars or species.https://www.frontiersin.org/articles/10.3389/fpls.2023.1216217/fullresistancerootstockscionsharkatrans-grafting |
| spellingShingle | Nuria Alburquerque Cristian Pérez-Caselles Lydia Faize Vincenza Ilardi Lorenzo Burgos Trans-grafting plum pox virus resistance from transgenic plum rootstocks to apricot scions Frontiers in Plant Science resistance rootstock scion sharka trans-grafting |
| title | Trans-grafting plum pox virus resistance from transgenic plum rootstocks to apricot scions |
| title_full | Trans-grafting plum pox virus resistance from transgenic plum rootstocks to apricot scions |
| title_fullStr | Trans-grafting plum pox virus resistance from transgenic plum rootstocks to apricot scions |
| title_full_unstemmed | Trans-grafting plum pox virus resistance from transgenic plum rootstocks to apricot scions |
| title_short | Trans-grafting plum pox virus resistance from transgenic plum rootstocks to apricot scions |
| title_sort | trans grafting plum pox virus resistance from transgenic plum rootstocks to apricot scions |
| topic | resistance rootstock scion sharka trans-grafting |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1216217/full |
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