Temperature stability of urinary F2-isoprostane and 8-hydroxy-2′-deoxyguanosine
Background: Clinical and epidemiological studies employ long-term temperature storage but the effect of temperature on the stability of oxidative stress (OS) markers is unknown. We investigated the effects of storage at −20 °C and −80 °C over 4–9 months on F2-isoprostanes (F2-IsoP) and 8-hydroxy-2′-...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Practical Laboratory Medicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352551724000192 |
| _version_ | 1797299678438490112 |
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| author | Katarzyna Kordas Diala Ghazal Elena I. Queirolo James R. Olson María Inés Beledo Richard W. Browne |
| author_facet | Katarzyna Kordas Diala Ghazal Elena I. Queirolo James R. Olson María Inés Beledo Richard W. Browne |
| author_sort | Katarzyna Kordas |
| collection | DOAJ |
| description | Background: Clinical and epidemiological studies employ long-term temperature storage but the effect of temperature on the stability of oxidative stress (OS) markers is unknown. We investigated the effects of storage at −20 °C and −80 °C over 4–9 months on F2-isoprostanes (F2-IsoP) and 8-hydroxy-2′-deoxyguanosine (8-OHdG) levels in urine of children, a population group among whom the measurement of these markers is still limited. Methods: Paired spot urine samples from 87 children aged 8.9–16.9 years (52.9% boys) were analyzed. Samples were preserved with 0.005% (w/v) butylated hydroxytoluene, portioned and stored within 2.5 h (median) of collection. Samples were analyzed in duplicate or triplicate using commercial ELISA kits and their correlations were evaluated. Results: F2-IsoP and 8-OHdG showed high correlations (Spearman rho of 0.90 and 0.97, respectively; P < 0.0001) with storage at −20 °C and −80 °C. There was a strong agreement among categories of values for F2-IsoP (Kappa = 0.76 ± 0.08, agreement = 83.9%, P < 0.0001) and 8-OHdG: (Kappa = 0.83 ± 0.08, agreement = 88.4%, P < 0.0001). The correlation between the temperatures for F2-IsoP concentrations was also high when stored for <4 (0.93), 4 (0.93), and 5 months (0.88), all P < 0.0001. For 8-OHdG, Spearman correlations at <8, 8, and 9 months of storage at −20 °C and −80 °C were 0.95, 0.98, and 0.96 (all P < 0.0001), respectively. Conclusions: Urine storage with BHT for up to nine months at a temperature of −20 °C to −80 °C yields highly comparable concentrations of F2-IsoP and 8-OHdG. |
| first_indexed | 2024-03-07T22:54:09Z |
| format | Article |
| id | doaj.art-45b4867910c347ed8f33a4c2dce91660 |
| institution | Directory Open Access Journal |
| issn | 2352-5517 |
| language | English |
| last_indexed | 2024-03-07T22:54:09Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Practical Laboratory Medicine |
| spelling | doaj.art-45b4867910c347ed8f33a4c2dce916602024-02-23T05:00:07ZengElsevierPractical Laboratory Medicine2352-55172024-03-0139e00373Temperature stability of urinary F2-isoprostane and 8-hydroxy-2′-deoxyguanosineKatarzyna Kordas0Diala Ghazal1Elena I. Queirolo2James R. Olson3María Inés Beledo4Richard W. Browne5Department of Epidemiology and Environmental Health, 270 Farber Hall, University at Buffalo, Buffalo, NY, 14214, USA; Corresponding author.Department of Biotechnical and Clinical Laboratory Sciences, University at Buffalo, Buffalo, NY, USADepartment of Neuroscience and Learning, Faculty of Health Sciences, Catholic University of Uruguay, Montevideo, UruguayDepartment of Epidemiology and Environmental Health, 270 Farber Hall, University at Buffalo, Buffalo, NY, 14214, USADepartment of Neuroscience and Learning, Faculty of Health Sciences, Catholic University of Uruguay, Montevideo, UruguayDepartment of Biotechnical and Clinical Laboratory Sciences, University at Buffalo, Buffalo, NY, USABackground: Clinical and epidemiological studies employ long-term temperature storage but the effect of temperature on the stability of oxidative stress (OS) markers is unknown. We investigated the effects of storage at −20 °C and −80 °C over 4–9 months on F2-isoprostanes (F2-IsoP) and 8-hydroxy-2′-deoxyguanosine (8-OHdG) levels in urine of children, a population group among whom the measurement of these markers is still limited. Methods: Paired spot urine samples from 87 children aged 8.9–16.9 years (52.9% boys) were analyzed. Samples were preserved with 0.005% (w/v) butylated hydroxytoluene, portioned and stored within 2.5 h (median) of collection. Samples were analyzed in duplicate or triplicate using commercial ELISA kits and their correlations were evaluated. Results: F2-IsoP and 8-OHdG showed high correlations (Spearman rho of 0.90 and 0.97, respectively; P < 0.0001) with storage at −20 °C and −80 °C. There was a strong agreement among categories of values for F2-IsoP (Kappa = 0.76 ± 0.08, agreement = 83.9%, P < 0.0001) and 8-OHdG: (Kappa = 0.83 ± 0.08, agreement = 88.4%, P < 0.0001). The correlation between the temperatures for F2-IsoP concentrations was also high when stored for <4 (0.93), 4 (0.93), and 5 months (0.88), all P < 0.0001. For 8-OHdG, Spearman correlations at <8, 8, and 9 months of storage at −20 °C and −80 °C were 0.95, 0.98, and 0.96 (all P < 0.0001), respectively. Conclusions: Urine storage with BHT for up to nine months at a temperature of −20 °C to −80 °C yields highly comparable concentrations of F2-IsoP and 8-OHdG.http://www.sciencedirect.com/science/article/pii/S2352551724000192UrineOxidative stress markersStabilityTemperature |
| spellingShingle | Katarzyna Kordas Diala Ghazal Elena I. Queirolo James R. Olson María Inés Beledo Richard W. Browne Temperature stability of urinary F2-isoprostane and 8-hydroxy-2′-deoxyguanosine Practical Laboratory Medicine Urine Oxidative stress markers Stability Temperature |
| title | Temperature stability of urinary F2-isoprostane and 8-hydroxy-2′-deoxyguanosine |
| title_full | Temperature stability of urinary F2-isoprostane and 8-hydroxy-2′-deoxyguanosine |
| title_fullStr | Temperature stability of urinary F2-isoprostane and 8-hydroxy-2′-deoxyguanosine |
| title_full_unstemmed | Temperature stability of urinary F2-isoprostane and 8-hydroxy-2′-deoxyguanosine |
| title_short | Temperature stability of urinary F2-isoprostane and 8-hydroxy-2′-deoxyguanosine |
| title_sort | temperature stability of urinary f2 isoprostane and 8 hydroxy 2 deoxyguanosine |
| topic | Urine Oxidative stress markers Stability Temperature |
| url | http://www.sciencedirect.com/science/article/pii/S2352551724000192 |
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