Patches and Blebs: A Comparative Study of the Composition and Biophysical Properties of Two Plasma Membrane Preparations from CHO Cells
This study was aimed at preparing and characterizing plasma membranes (PM) from Chinese Hamster Ovary (CHO) cells. Two methods of PM preparation were applied, one based on adhering cells to a poly-lysine-coated surface, followed by hypotonic lysis and removal of intracellular components, so that PM...
Main Authors: | Bingen G. Monasterio, Noemi Jiménez-Rojo, Aritz B. García-Arribas, Howard Riezman, Félix M. Goñi, Alicia Alonso |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2020-04-01
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Series: | International Journal of Molecular Sciences |
Subjects: | |
Online Access: | https://www.mdpi.com/1422-0067/21/7/2643 |
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