The role of alveolar epithelium in radiation-induced lung injury.
Pneumonitis and fibrosis are major lung complications of irradiating thoracic malignancies. In the current study, we determined the effect of thoracic irradiation on the lungs of FVB/N mice. Survival data showed a dose-dependent increase in morbidity following thoracic irradiation with single (11-13...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3543315?pdf=render |
| _version_ | 1811213489796022272 |
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| author | Celine Almeida Devipriya Nagarajan Jian Tian Sofia Walder Leal Kenneth Wheeler Michael Munley William Blackstock Weiling Zhao |
| author_facet | Celine Almeida Devipriya Nagarajan Jian Tian Sofia Walder Leal Kenneth Wheeler Michael Munley William Blackstock Weiling Zhao |
| author_sort | Celine Almeida |
| collection | DOAJ |
| description | Pneumonitis and fibrosis are major lung complications of irradiating thoracic malignancies. In the current study, we determined the effect of thoracic irradiation on the lungs of FVB/N mice. Survival data showed a dose-dependent increase in morbidity following thoracic irradiation with single (11-13 Gy) and fractionated doses (24-36 Gy) of (137)Cs γ-rays. Histological examination showed a thickening of vessel walls, accumulation of inflammatory cells, collagen deposition, and regional fibrosis in the lungs 14 weeks after a single 12 Gy dose and a fractionated 30 Gy dose; this damage was also seen 5 months after a fractionated 24 Gy dose. After both single and fractionated doses, i] aquaporin-5 was markedly decreased, ii] E-cadherin was reduced and iii] prosurfactant Protein C (pro-SP-c), the number of pro-SP-c(+) cells and vimentin expression were increased in the lungs. Immunofluorescence analysis revealed co-localization of pro-SP-c and α-smooth muscle actin in the alveoli after a single dose of 12 Gy. These data suggest that, i] the FVB/N mouse strain is sensitive to thoracic radiation ii] aquaporin-5, E-cadherin, and pro-SP-c may serve as sensitive indicators of radiation-induced lung injury; and iii] the epithelial-to-mesenchymal transition may play an important role in the development of radiation-induced lung fibrosis. |
| first_indexed | 2024-04-12T05:46:09Z |
| format | Article |
| id | doaj.art-45c1bf260ba24096ba2df68110df16f8 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T05:46:09Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-45c1bf260ba24096ba2df68110df16f82022-12-22T03:45:26ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0181e5362810.1371/journal.pone.0053628The role of alveolar epithelium in radiation-induced lung injury.Celine AlmeidaDevipriya NagarajanJian TianSofia Walder LealKenneth WheelerMichael MunleyWilliam BlackstockWeiling ZhaoPneumonitis and fibrosis are major lung complications of irradiating thoracic malignancies. In the current study, we determined the effect of thoracic irradiation on the lungs of FVB/N mice. Survival data showed a dose-dependent increase in morbidity following thoracic irradiation with single (11-13 Gy) and fractionated doses (24-36 Gy) of (137)Cs γ-rays. Histological examination showed a thickening of vessel walls, accumulation of inflammatory cells, collagen deposition, and regional fibrosis in the lungs 14 weeks after a single 12 Gy dose and a fractionated 30 Gy dose; this damage was also seen 5 months after a fractionated 24 Gy dose. After both single and fractionated doses, i] aquaporin-5 was markedly decreased, ii] E-cadherin was reduced and iii] prosurfactant Protein C (pro-SP-c), the number of pro-SP-c(+) cells and vimentin expression were increased in the lungs. Immunofluorescence analysis revealed co-localization of pro-SP-c and α-smooth muscle actin in the alveoli after a single dose of 12 Gy. These data suggest that, i] the FVB/N mouse strain is sensitive to thoracic radiation ii] aquaporin-5, E-cadherin, and pro-SP-c may serve as sensitive indicators of radiation-induced lung injury; and iii] the epithelial-to-mesenchymal transition may play an important role in the development of radiation-induced lung fibrosis.http://europepmc.org/articles/PMC3543315?pdf=render |
| spellingShingle | Celine Almeida Devipriya Nagarajan Jian Tian Sofia Walder Leal Kenneth Wheeler Michael Munley William Blackstock Weiling Zhao The role of alveolar epithelium in radiation-induced lung injury. PLoS ONE |
| title | The role of alveolar epithelium in radiation-induced lung injury. |
| title_full | The role of alveolar epithelium in radiation-induced lung injury. |
| title_fullStr | The role of alveolar epithelium in radiation-induced lung injury. |
| title_full_unstemmed | The role of alveolar epithelium in radiation-induced lung injury. |
| title_short | The role of alveolar epithelium in radiation-induced lung injury. |
| title_sort | role of alveolar epithelium in radiation induced lung injury |
| url | http://europepmc.org/articles/PMC3543315?pdf=render |
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