Changes and significance of leukotriene C4 and type 2 innate lymphoid cell in bronchopulmonary dysplasia
Objective To investigate the dynamic changes of leukotriene C4 (LTC4), type 2 innate lymphoid cell(ILC2) and related cytokines (IL-4, IL-5 and IL-13) in the lung tissue of mice with bronchopulmonary dysplasia (BPD). Methods C57BL / 6 mice were randomly divided into air group and hyperoxia group.Th...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2023-05-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2023-43-5-711.pdf |
| _version_ | 1797366342070829056 |
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| author | JI Wei, ZHU Yue, YAO Huici, LU Hongyan |
| author_facet | JI Wei, ZHU Yue, YAO Huici, LU Hongyan |
| author_sort | JI Wei, ZHU Yue, YAO Huici, LU Hongyan |
| collection | DOAJ |
| description | Objective To investigate the dynamic changes of leukotriene C4 (LTC4), type 2 innate lymphoid cell(ILC2) and related cytokines (IL-4, IL-5 and IL-13) in the lung tissue of mice with bronchopulmonary dysplasia (BPD). Methods C57BL / 6 mice were randomly divided into air group and hyperoxia group.The hyperoxia group was exposed to 85% oxygen for 1, 7 and 14 days to replicate the BPD model.HE staining microscopy was used to observe the pathological changes of the lung tissue of mice. The percentages of ILC2 and CysLT1R+ILC2 were detected by flow cytometry.The contents of LTC4, IL-4, IL-5 and IL-13 were detected by ELISA.Western blot was used to detect the expression of LTC4-C synthase (LTC4S).RT-qPCR was used to detect the mRNA expression of LTC4S and cysteine leukotriene type 1 receptor (CysLT1R). Results Compared with air group, the percentage of ILC2 and CysLT1R+ILC2 in hyperoxia group and the expression of CysLT1R mRNA all increased(P＜0.05);The expression of IL-4, IL-5 and IL-13 in the hyperoxia group increased and were higher than those in the air group at the same time point (P＜0.05);LTC4 content, LTC4S protein and mRNA expression were significantly increased in hyperoxia group (P＜0.05). Conclusions In BPD, LTC4 increases and may induce the increase of ILC2 and related cytokines (IL-4, IL-5 and IL-13) through CysLT1R. |
| first_indexed | 2024-03-08T17:02:51Z |
| format | Article |
| id | doaj.art-45c82eb59a264eacb40b67d5e2f1556d |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T17:02:51Z |
| publishDate | 2023-05-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-45c82eb59a264eacb40b67d5e2f1556d2024-01-04T07:32:51ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252023-05-0143571171610.16352/j.issn.1001-6325.2023.05.0711Changes and significance of leukotriene C4 and type 2 innate lymphoid cell in bronchopulmonary dysplasiaJI Wei, ZHU Yue, YAO Huici, LU Hongyan0Department of Pediatrics, Affiliated Hospital of Jiangsu University,Zhenjiang 212001, ChinaObjective To investigate the dynamic changes of leukotriene C4 (LTC4), type 2 innate lymphoid cell(ILC2) and related cytokines (IL-4, IL-5 and IL-13) in the lung tissue of mice with bronchopulmonary dysplasia (BPD). Methods C57BL / 6 mice were randomly divided into air group and hyperoxia group.The hyperoxia group was exposed to 85% oxygen for 1, 7 and 14 days to replicate the BPD model.HE staining microscopy was used to observe the pathological changes of the lung tissue of mice. The percentages of ILC2 and CysLT1R+ILC2 were detected by flow cytometry.The contents of LTC4, IL-4, IL-5 and IL-13 were detected by ELISA.Western blot was used to detect the expression of LTC4-C synthase (LTC4S).RT-qPCR was used to detect the mRNA expression of LTC4S and cysteine leukotriene type 1 receptor (CysLT1R). Results Compared with air group, the percentage of ILC2 and CysLT1R+ILC2 in hyperoxia group and the expression of CysLT1R mRNA all increased(P＜0.05);The expression of IL-4, IL-5 and IL-13 in the hyperoxia group increased and were higher than those in the air group at the same time point (P＜0.05);LTC4 content, LTC4S protein and mRNA expression were significantly increased in hyperoxia group (P＜0.05). Conclusions In BPD, LTC4 increases and may induce the increase of ILC2 and related cytokines (IL-4, IL-5 and IL-13) through CysLT1R.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2023-43-5-711.pdfleukotriene c4|type 2 innate lymphocytes|type 2 inflammatory response|bronchopulmonary dysplasia |
| spellingShingle | JI Wei, ZHU Yue, YAO Huici, LU Hongyan Changes and significance of leukotriene C4 and type 2 innate lymphoid cell in bronchopulmonary dysplasia Jichu yixue yu linchuang leukotriene c4|type 2 innate lymphocytes|type 2 inflammatory response|bronchopulmonary dysplasia |
| title | Changes and significance of leukotriene C4 and type 2 innate lymphoid cell in bronchopulmonary dysplasia |
| title_full | Changes and significance of leukotriene C4 and type 2 innate lymphoid cell in bronchopulmonary dysplasia |
| title_fullStr | Changes and significance of leukotriene C4 and type 2 innate lymphoid cell in bronchopulmonary dysplasia |
| title_full_unstemmed | Changes and significance of leukotriene C4 and type 2 innate lymphoid cell in bronchopulmonary dysplasia |
| title_short | Changes and significance of leukotriene C4 and type 2 innate lymphoid cell in bronchopulmonary dysplasia |
| title_sort | changes and significance of leukotriene c4 and type 2 innate lymphoid cell in bronchopulmonary dysplasia |
| topic | leukotriene c4|type 2 innate lymphocytes|type 2 inflammatory response|bronchopulmonary dysplasia |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2023-43-5-711.pdf |
| work_keys_str_mv | AT jiweizhuyueyaohuiciluhongyan changesandsignificanceofleukotrienec4andtype2innatelymphoidcellinbronchopulmonarydysplasia |