Ginsenoside Rg1 Ameliorates Rat Myocardial Ischemia-Reperfusion Injury by Modulating Energy Metabolism Pathways
As a major ingredient of Radix ginseng, ginsenoside Rg1 (Rg1) has been increasingly recognized to benefit the heart condition, however, the rationale behind the role is not fully understood. In vitro study in H9c2 cardiomyocytes has shown the potential of Rg1 to increase ATP content in the cells. We...
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Frontiers Media S.A.
2018-02-01
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fphys.2018.00078/full |
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| author | Lin Li Lin Li Chun-Shui Pan Chun-Shui Pan Chun-Shui Pan Chun-Shui Pan Chun-Shui Pan Li Yan Li Yan Li Yan Li Yan Li Yan Yuan-Chen Cui Yuan-Chen Cui Yuan-Chen Cui Yuan-Chen Cui Yuan-Chen Cui Yu-Ying Liu Yu-Ying Liu Yu-Ying Liu Yu-Ying Liu Yu-Ying Liu Hong-Na Mu Hong-Na Mu Hong-Na Mu Hong-Na Mu Hong-Na Mu Ke He Ke He Ke He Ke He Ke He Bai-He Hu Bai-He Hu Bai-He Hu Bai-He Hu Bai-He Hu Xin Chang Xin Chang Xin Chang Xin Chang Xin Chang Kai Sun Kai Sun Kai Sun Kai Sun Kai Sun Jing-Yu Fan Jing-Yu Fan Jing-Yu Fan Jing-Yu Fan Jing-Yu Fan Li Huang Jing-Yan Han Jing-Yan Han Jing-Yan Han Jing-Yan Han Jing-Yan Han Jing-Yan Han |
| author_facet | Lin Li Lin Li Chun-Shui Pan Chun-Shui Pan Chun-Shui Pan Chun-Shui Pan Chun-Shui Pan Li Yan Li Yan Li Yan Li Yan Li Yan Yuan-Chen Cui Yuan-Chen Cui Yuan-Chen Cui Yuan-Chen Cui Yuan-Chen Cui Yu-Ying Liu Yu-Ying Liu Yu-Ying Liu Yu-Ying Liu Yu-Ying Liu Hong-Na Mu Hong-Na Mu Hong-Na Mu Hong-Na Mu Hong-Na Mu Ke He Ke He Ke He Ke He Ke He Bai-He Hu Bai-He Hu Bai-He Hu Bai-He Hu Bai-He Hu Xin Chang Xin Chang Xin Chang Xin Chang Xin Chang Kai Sun Kai Sun Kai Sun Kai Sun Kai Sun Jing-Yu Fan Jing-Yu Fan Jing-Yu Fan Jing-Yu Fan Jing-Yu Fan Li Huang Jing-Yan Han Jing-Yan Han Jing-Yan Han Jing-Yan Han Jing-Yan Han Jing-Yan Han |
| author_sort | Lin Li |
| collection | DOAJ |
| description | As a major ingredient of Radix ginseng, ginsenoside Rg1 (Rg1) has been increasingly recognized to benefit the heart condition, however, the rationale behind the role is not fully understood. In vitro study in H9c2 cardiomyocytes has shown the potential of Rg1 to increase ATP content in the cells. We thus speculated that the protective effect of Rg1 on heart ischemia and reperfusion (I/R) injury implicates energy metabolism regulation. The present study was designed to verify this speculation. Male Sprague-Dawley rats were subjected to 30 min of occlusion of left coronary anterior descending artery followed by reperfusion for 90 min. Rg1 (5 mg/kg/h) was continuously administrated intravenously 30 min before occlusion until the end of reperfusion. Myocradial blood flow and heart function were monitored over the period of I/R. Myocardial infarct size, structure and apoptosis, energy metabolism, and change in RhoA signaling pathway were evaluated 90 min after reperfusion. Binding of Rg1 to RhoA was assessed using Surface Plasmon Resonance (SPR). Rg1 prevented I/R-elicited insults in myocardium, including myocardial infarction and apoptosis, decreased myocardial blood flow (MBF) and heart function, and alteration in myocardium structure. Rg1 restored the production of ATP in myocardium after I/R. Rg1 was able to bind to RhoA and down-regulate the activity of RhoA signaling pathway. These results indicated that Rg1 had protective potential against I/R-induced myocardial injury, which may be related to inhibiting myocardial apoptosis and modulating energy metabolism through binding to RhoA. |
| first_indexed | 2024-12-11T13:49:54Z |
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| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-11T13:49:54Z |
| publishDate | 2018-02-01 |
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| series | Frontiers in Physiology |
| spelling | doaj.art-45cb81588a0f446faafc26663a77ddf72022-12-22T01:04:19ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2018-02-01910.3389/fphys.2018.00078329120Ginsenoside Rg1 Ameliorates Rat Myocardial Ischemia-Reperfusion Injury by Modulating Energy Metabolism PathwaysLin Li0Lin Li1Chun-Shui Pan2Chun-Shui Pan3Chun-Shui Pan4Chun-Shui Pan5Chun-Shui Pan6Li Yan7Li Yan8Li Yan9Li Yan10Li Yan11Yuan-Chen Cui12Yuan-Chen Cui13Yuan-Chen Cui14Yuan-Chen Cui15Yuan-Chen Cui16Yu-Ying Liu17Yu-Ying Liu18Yu-Ying Liu19Yu-Ying Liu20Yu-Ying Liu21Hong-Na Mu22Hong-Na Mu23Hong-Na Mu24Hong-Na Mu25Hong-Na Mu26Ke He27Ke He28Ke He29Ke He30Ke He31Bai-He Hu32Bai-He Hu33Bai-He Hu34Bai-He Hu35Bai-He Hu36Xin Chang37Xin Chang38Xin Chang39Xin Chang40Xin Chang41Kai Sun42Kai Sun43Kai Sun44Kai Sun45Kai Sun46Jing-Yu Fan47Jing-Yu Fan48Jing-Yu Fan49Jing-Yu Fan50Jing-Yu Fan51Li Huang52Jing-Yan Han53Jing-Yan Han54Jing-Yan Han55Jing-Yan Han56Jing-Yan Han57Jing-Yan Han58Department of Integrative Cardiology, Beijing China-Japan Friendship Hospital, Beijing, ChinaTasly Microcirculation Research Center, Peking University Health Science Center, Beijing, ChinaTasly Microcirculation Research Center, Peking University Health Science Center, Beijing, ChinaKey Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, ChinaKey Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, ChinaBeijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, ChinaState Key Laboratory of Core Technology in Innovative Chinese Medicine, Beijing, ChinaTasly Microcirculation Research Center, Peking University Health Science Center, Beijing, ChinaKey Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, ChinaKey Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, ChinaBeijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, ChinaState Key Laboratory of Core Technology in Innovative Chinese Medicine, Beijing, ChinaTasly Microcirculation Research Center, Peking University Health Science Center, Beijing, ChinaKey Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, ChinaKey Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, ChinaBeijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, ChinaState Key Laboratory of Core Technology in Innovative Chinese Medicine, Beijing, ChinaTasly Microcirculation Research Center, Peking University Health Science Center, Beijing, ChinaKey Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, ChinaKey Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, ChinaBeijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, ChinaState Key Laboratory of Core Technology in Innovative Chinese Medicine, Beijing, ChinaTasly Microcirculation Research Center, Peking University Health Science Center, Beijing, ChinaKey Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, ChinaKey Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, ChinaBeijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, ChinaState Key Laboratory of Core Technology in Innovative Chinese Medicine, Beijing, ChinaTasly Microcirculation Research Center, Peking University Health Science Center, Beijing, ChinaKey Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, ChinaKey Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, ChinaBeijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, ChinaState Key Laboratory of Core Technology in Innovative Chinese Medicine, Beijing, ChinaTasly Microcirculation Research Center, Peking University Health Science Center, Beijing, ChinaKey Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, ChinaKey Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, ChinaBeijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, ChinaState Key Laboratory of Core Technology in Innovative Chinese Medicine, Beijing, ChinaTasly Microcirculation Research Center, Peking University Health Science Center, Beijing, ChinaKey Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, ChinaKey Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, ChinaBeijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, ChinaState Key Laboratory of Core Technology in Innovative Chinese Medicine, Beijing, ChinaTasly Microcirculation Research Center, Peking University Health Science Center, Beijing, ChinaKey Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, ChinaKey Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, ChinaBeijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, ChinaState Key Laboratory of Core Technology in Innovative Chinese Medicine, Beijing, ChinaTasly Microcirculation Research Center, Peking University Health Science Center, Beijing, ChinaKey Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, ChinaKey Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, ChinaBeijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, ChinaState Key Laboratory of Core Technology in Innovative Chinese Medicine, Beijing, ChinaDepartment of Integrative Cardiology, Beijing China-Japan Friendship Hospital, Beijing, ChinaTasly Microcirculation Research Center, Peking University Health Science Center, Beijing, ChinaKey Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, ChinaKey Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, ChinaBeijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, ChinaState Key Laboratory of Core Technology in Innovative Chinese Medicine, Beijing, ChinaDepartment of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, ChinaAs a major ingredient of Radix ginseng, ginsenoside Rg1 (Rg1) has been increasingly recognized to benefit the heart condition, however, the rationale behind the role is not fully understood. In vitro study in H9c2 cardiomyocytes has shown the potential of Rg1 to increase ATP content in the cells. We thus speculated that the protective effect of Rg1 on heart ischemia and reperfusion (I/R) injury implicates energy metabolism regulation. The present study was designed to verify this speculation. Male Sprague-Dawley rats were subjected to 30 min of occlusion of left coronary anterior descending artery followed by reperfusion for 90 min. Rg1 (5 mg/kg/h) was continuously administrated intravenously 30 min before occlusion until the end of reperfusion. Myocradial blood flow and heart function were monitored over the period of I/R. Myocardial infarct size, structure and apoptosis, energy metabolism, and change in RhoA signaling pathway were evaluated 90 min after reperfusion. Binding of Rg1 to RhoA was assessed using Surface Plasmon Resonance (SPR). Rg1 prevented I/R-elicited insults in myocardium, including myocardial infarction and apoptosis, decreased myocardial blood flow (MBF) and heart function, and alteration in myocardium structure. Rg1 restored the production of ATP in myocardium after I/R. Rg1 was able to bind to RhoA and down-regulate the activity of RhoA signaling pathway. These results indicated that Rg1 had protective potential against I/R-induced myocardial injury, which may be related to inhibiting myocardial apoptosis and modulating energy metabolism through binding to RhoA.http://journal.frontiersin.org/article/10.3389/fphys.2018.00078/fullpanax notoginsengcardiac functionATP synthase subunitRhoAenergy metabolism |
| spellingShingle | Lin Li Lin Li Chun-Shui Pan Chun-Shui Pan Chun-Shui Pan Chun-Shui Pan Chun-Shui Pan Li Yan Li Yan Li Yan Li Yan Li Yan Yuan-Chen Cui Yuan-Chen Cui Yuan-Chen Cui Yuan-Chen Cui Yuan-Chen Cui Yu-Ying Liu Yu-Ying Liu Yu-Ying Liu Yu-Ying Liu Yu-Ying Liu Hong-Na Mu Hong-Na Mu Hong-Na Mu Hong-Na Mu Hong-Na Mu Ke He Ke He Ke He Ke He Ke He Bai-He Hu Bai-He Hu Bai-He Hu Bai-He Hu Bai-He Hu Xin Chang Xin Chang Xin Chang Xin Chang Xin Chang Kai Sun Kai Sun Kai Sun Kai Sun Kai Sun Jing-Yu Fan Jing-Yu Fan Jing-Yu Fan Jing-Yu Fan Jing-Yu Fan Li Huang Jing-Yan Han Jing-Yan Han Jing-Yan Han Jing-Yan Han Jing-Yan Han Jing-Yan Han Ginsenoside Rg1 Ameliorates Rat Myocardial Ischemia-Reperfusion Injury by Modulating Energy Metabolism Pathways Frontiers in Physiology panax notoginseng cardiac function ATP synthase subunit RhoA energy metabolism |
| title | Ginsenoside Rg1 Ameliorates Rat Myocardial Ischemia-Reperfusion Injury by Modulating Energy Metabolism Pathways |
| title_full | Ginsenoside Rg1 Ameliorates Rat Myocardial Ischemia-Reperfusion Injury by Modulating Energy Metabolism Pathways |
| title_fullStr | Ginsenoside Rg1 Ameliorates Rat Myocardial Ischemia-Reperfusion Injury by Modulating Energy Metabolism Pathways |
| title_full_unstemmed | Ginsenoside Rg1 Ameliorates Rat Myocardial Ischemia-Reperfusion Injury by Modulating Energy Metabolism Pathways |
| title_short | Ginsenoside Rg1 Ameliorates Rat Myocardial Ischemia-Reperfusion Injury by Modulating Energy Metabolism Pathways |
| title_sort | ginsenoside rg1 ameliorates rat myocardial ischemia reperfusion injury by modulating energy metabolism pathways |
| topic | panax notoginseng cardiac function ATP synthase subunit RhoA energy metabolism |
| url | http://journal.frontiersin.org/article/10.3389/fphys.2018.00078/full |
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