Effect of [10]-Gingerol on [Ca2+]i and Cell Death in Human Colorectal Cancer Cells
The effect of [10]-gingerol on cytosol free Ca2+ concentration ([Ca2+]i) and viability is large unknown. This study examines the early signaling effects of [10]-gingerol on human colorectal cancer cells. It was found that this compound caused a slow and sustained rise of [Ca2+]i in a concentration-d...
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| Format: | Article |
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MDPI AG
2009-03-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/14/3/959/ |
| _version_ | 1818541639172358144 |
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| author | Chung-Yi Chen Yi-Wen Li Soong-Yu Kuo |
| author_facet | Chung-Yi Chen Yi-Wen Li Soong-Yu Kuo |
| author_sort | Chung-Yi Chen |
| collection | DOAJ |
| description | The effect of [10]-gingerol on cytosol free Ca2+ concentration ([Ca2+]i) and viability is large unknown. This study examines the early signaling effects of [10]-gingerol on human colorectal cancer cells. It was found that this compound caused a slow and sustained rise of [Ca2+]i in a concentration-dependent manner. [10]-Gingerol also induced a [Ca2+]i rise when extracellular Ca2+ was removed, but the magnitude was reduced by 38%. In a Ca2+-free medium, the [10]-gingerol-induced [Ca2+]i rise was partially abolished by depleting stored Ca2+ with thapsigargin (an endoplasmic reticulum Ca2+ pump inhibitor). The elevation of [10]-gingerol-caused [Ca2+]i in a Ca2+-containing medium was not affected by modulation of protein kinase C activity. The [10]-gingerol-induced Ca2+ influx was insensitive to L-type Ca2+ channel blockers. At concentrations of 10-100 mM, [10]-gingerol killed cells in a concentration-dependent manner. These findings suggest that [10]-gingerol induces [Ca2+]i rise by causing Ca2+ release from the endoplasmic reticulum and Ca2+ influx from non-L-type Ca2+ channels in SW480 cancer cells. |
| first_indexed | 2024-12-11T22:11:53Z |
| format | Article |
| id | doaj.art-45cc2188b5b9427199fceae7906bae2c |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-11T22:11:53Z |
| publishDate | 2009-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-45cc2188b5b9427199fceae7906bae2c2022-12-22T00:48:44ZengMDPI AGMolecules1420-30492009-03-0114395996910.3390/molecules14030959Effect of [10]-Gingerol on [Ca2+]i and Cell Death in Human Colorectal Cancer CellsChung-Yi ChenYi-Wen LiSoong-Yu KuoThe effect of [10]-gingerol on cytosol free Ca2+ concentration ([Ca2+]i) and viability is large unknown. This study examines the early signaling effects of [10]-gingerol on human colorectal cancer cells. It was found that this compound caused a slow and sustained rise of [Ca2+]i in a concentration-dependent manner. [10]-Gingerol also induced a [Ca2+]i rise when extracellular Ca2+ was removed, but the magnitude was reduced by 38%. In a Ca2+-free medium, the [10]-gingerol-induced [Ca2+]i rise was partially abolished by depleting stored Ca2+ with thapsigargin (an endoplasmic reticulum Ca2+ pump inhibitor). The elevation of [10]-gingerol-caused [Ca2+]i in a Ca2+-containing medium was not affected by modulation of protein kinase C activity. The [10]-gingerol-induced Ca2+ influx was insensitive to L-type Ca2+ channel blockers. At concentrations of 10-100 mM, [10]-gingerol killed cells in a concentration-dependent manner. These findings suggest that [10]-gingerol induces [Ca2+]i rise by causing Ca2+ release from the endoplasmic reticulum and Ca2+ influx from non-L-type Ca2+ channels in SW480 cancer cells.http://www.mdpi.com/1420-3049/14/3/959/Ca2+[10]-GingerolL-type Ca2+ channel blockersSW480 cellsThapsigargin |
| spellingShingle | Chung-Yi Chen Yi-Wen Li Soong-Yu Kuo Effect of [10]-Gingerol on [Ca2+]i and Cell Death in Human Colorectal Cancer Cells Molecules Ca2+ [10]-Gingerol L-type Ca2+ channel blockers SW480 cells Thapsigargin |
| title | Effect of [10]-Gingerol on [Ca2+]i and Cell Death in Human Colorectal Cancer Cells |
| title_full | Effect of [10]-Gingerol on [Ca2+]i and Cell Death in Human Colorectal Cancer Cells |
| title_fullStr | Effect of [10]-Gingerol on [Ca2+]i and Cell Death in Human Colorectal Cancer Cells |
| title_full_unstemmed | Effect of [10]-Gingerol on [Ca2+]i and Cell Death in Human Colorectal Cancer Cells |
| title_short | Effect of [10]-Gingerol on [Ca2+]i and Cell Death in Human Colorectal Cancer Cells |
| title_sort | effect of 10 gingerol on ca2 i and cell death in human colorectal cancer cells |
| topic | Ca2+ [10]-Gingerol L-type Ca2+ channel blockers SW480 cells Thapsigargin |
| url | http://www.mdpi.com/1420-3049/14/3/959/ |
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