Development and characterization of novel microsatellite markers by Next Generation Sequencing for the blue and red shrimp Aristeus antennatus
The blue and red shrimp, Aristeus antennatus, is a commercially important crustacean, in the Mediterranean Sea, which has been listed as a priority species for fishery management. Hypervariable microsatellite markers could be a useful tool to identify genetic stocks among geographically close fishin...
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| Format: | Article |
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PeerJ Inc.
2016-07-01
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| Series: | PeerJ |
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| Online Access: | https://peerj.com/articles/2200.pdf |
| _version_ | 1797422154286891008 |
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| author | Sandra Heras Laia Planella Ilaria Caldarazzo Manuel Vera José-Luis García-Marín Maria Ines Roldán |
| author_facet | Sandra Heras Laia Planella Ilaria Caldarazzo Manuel Vera José-Luis García-Marín Maria Ines Roldán |
| author_sort | Sandra Heras |
| collection | DOAJ |
| description | The blue and red shrimp, Aristeus antennatus, is a commercially important crustacean, in the Mediterranean Sea, which has been listed as a priority species for fishery management. Hypervariable microsatellite markers could be a useful tool to identify genetic stocks among geographically close fishing grounds. Potential microsatellite markers (97) identified from next-generation sequencing of an individual shrimp using a 454 GS Junior Pyrosequencer were tested on a preliminary panel of 15 individuals representing the four worldwide genetic stocks of the species from which 35 polymorphic loci were identified and used to characterize an additional 20 individuals from the Western Mediterranean Sea. In the Western Mediterranean sample, 32 out of 35 were polymorphic loci and the number of alleles per locus ranged from 2 to 14 and expected heterozygosity ranged from 0.050 to 0.968. No linkage disequilibrium was detected, indicating the independence of the loci. These novel microsatellites provide additional tools to address questions relating to genetic diversity, parentage studies and connectivity patterns of A. antennatus populations and help develop effective strategies to ensure long-term sustainability of this resource. |
| first_indexed | 2024-03-09T07:28:06Z |
| format | Article |
| id | doaj.art-45d52a68bb0a454594677e986c61f555 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:28:06Z |
| publishDate | 2016-07-01 |
| publisher | PeerJ Inc. |
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| spelling | doaj.art-45d52a68bb0a454594677e986c61f5552023-12-03T06:48:10ZengPeerJ Inc.PeerJ2167-83592016-07-014e220010.7717/peerj.2200Development and characterization of novel microsatellite markers by Next Generation Sequencing for the blue and red shrimp Aristeus antennatusSandra Heras0Laia Planella1Ilaria Caldarazzo2Manuel Vera3José-Luis García-Marín4Maria Ines Roldán5Biology Department, University of Girona, Girona, SpainBiology Department, University of Girona, Girona, SpainBiology Department, University of Girona, Girona, SpainBiology Department, University of Girona, Girona, SpainBiology Department, University of Girona, Girona, SpainBiology Department, University of Girona, Girona, SpainThe blue and red shrimp, Aristeus antennatus, is a commercially important crustacean, in the Mediterranean Sea, which has been listed as a priority species for fishery management. Hypervariable microsatellite markers could be a useful tool to identify genetic stocks among geographically close fishing grounds. Potential microsatellite markers (97) identified from next-generation sequencing of an individual shrimp using a 454 GS Junior Pyrosequencer were tested on a preliminary panel of 15 individuals representing the four worldwide genetic stocks of the species from which 35 polymorphic loci were identified and used to characterize an additional 20 individuals from the Western Mediterranean Sea. In the Western Mediterranean sample, 32 out of 35 were polymorphic loci and the number of alleles per locus ranged from 2 to 14 and expected heterozygosity ranged from 0.050 to 0.968. No linkage disequilibrium was detected, indicating the independence of the loci. These novel microsatellites provide additional tools to address questions relating to genetic diversity, parentage studies and connectivity patterns of A. antennatus populations and help develop effective strategies to ensure long-term sustainability of this resource.https://peerj.com/articles/2200.pdfAristeus antennatusNext-generation sequencingMicrosatellite lociPopulation geneticsGenetic stocksBlue and red shrimp |
| spellingShingle | Sandra Heras Laia Planella Ilaria Caldarazzo Manuel Vera José-Luis García-Marín Maria Ines Roldán Development and characterization of novel microsatellite markers by Next Generation Sequencing for the blue and red shrimp Aristeus antennatus PeerJ Aristeus antennatus Next-generation sequencing Microsatellite loci Population genetics Genetic stocks Blue and red shrimp |
| title | Development and characterization of novel microsatellite markers by Next Generation Sequencing for the blue and red shrimp Aristeus antennatus |
| title_full | Development and characterization of novel microsatellite markers by Next Generation Sequencing for the blue and red shrimp Aristeus antennatus |
| title_fullStr | Development and characterization of novel microsatellite markers by Next Generation Sequencing for the blue and red shrimp Aristeus antennatus |
| title_full_unstemmed | Development and characterization of novel microsatellite markers by Next Generation Sequencing for the blue and red shrimp Aristeus antennatus |
| title_short | Development and characterization of novel microsatellite markers by Next Generation Sequencing for the blue and red shrimp Aristeus antennatus |
| title_sort | development and characterization of novel microsatellite markers by next generation sequencing for the blue and red shrimp aristeus antennatus |
| topic | Aristeus antennatus Next-generation sequencing Microsatellite loci Population genetics Genetic stocks Blue and red shrimp |
| url | https://peerj.com/articles/2200.pdf |
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