eIF4E1 Regulates Arabidopsis Embryo Development and Root Growth by Interacting With RopGEF7
Eukaryotic translation initiation factor 4E1 (eIF4E1) is required for the initiation of protein synthesis. The biological function of eIF4E1 in plant–potyvirus interactions has been extensively studied. However, the role of eIF4E1 in Arabidopsis development remains unclear. In this study, we show th...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.938476/full |
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| author | Taibo Liu Taibo Liu Taibo Liu Qianyu Liu Qianyu Liu Qianyu Liu Zhen Yu Zhen Yu Zhen Yu Chunling Wang Chunling Wang Huafu Mai Huafu Mai Huafu Mai Guolan Liu Guolan Liu Ruijing Li Ruijing Li Gang Pang Gang Pang Gang Pang Dingwu Chen Dingwu Chen Huili Liu Huili Liu Huili Liu Jiangyi Yang Li-Zhen Tao Li-Zhen Tao Li-Zhen Tao |
| author_facet | Taibo Liu Taibo Liu Taibo Liu Qianyu Liu Qianyu Liu Qianyu Liu Zhen Yu Zhen Yu Zhen Yu Chunling Wang Chunling Wang Huafu Mai Huafu Mai Huafu Mai Guolan Liu Guolan Liu Ruijing Li Ruijing Li Gang Pang Gang Pang Gang Pang Dingwu Chen Dingwu Chen Huili Liu Huili Liu Huili Liu Jiangyi Yang Li-Zhen Tao Li-Zhen Tao Li-Zhen Tao |
| author_sort | Taibo Liu |
| collection | DOAJ |
| description | Eukaryotic translation initiation factor 4E1 (eIF4E1) is required for the initiation of protein synthesis. The biological function of eIF4E1 in plant–potyvirus interactions has been extensively studied. However, the role of eIF4E1 in Arabidopsis development remains unclear. In this study, we show that eIF4E1 is highly expressed in the embryo and root apical meristem. In addition, eIF4E1 expression is induced by auxin. eIF4E1 mutants show embryonic cell division defects and short primary roots, a result of reduced cell divisions. Furthermore, our results show that mutation in eIF4E1 severely reduces the accumulation of PIN-FORMED (PIN) proteins and decreases auxin-responsive gene expression at the root tip. Yeast two-hybrid assays identified that eIF4E1 interacts with an RAC/ROP GTPase activator, RopGEF7, which has been previously reported to be involved in the maintenance of the root apical meristem. The interaction between eIF4E1 and RopGEF7 is confirmed by protein pull-down and bimolecular fluorescent complementation assays in plant cells. Taken together, our results demonstrated that eIF4E1 is important for auxin-regulated embryo development and root growth. The eIF4E1–RopGEF7 interaction suggests that eIF4E1 may act through ROP signaling to regulate auxin transport, thus regulating auxin-dependent patterning. |
| first_indexed | 2024-12-12T07:50:17Z |
| format | Article |
| id | doaj.art-45d5f3e871064888a8b8151789095bdc |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-12T07:50:17Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-45d5f3e871064888a8b8151789095bdc2022-12-22T00:32:28ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-06-011310.3389/fpls.2022.938476938476eIF4E1 Regulates Arabidopsis Embryo Development and Root Growth by Interacting With RopGEF7Taibo Liu0Taibo Liu1Taibo Liu2Qianyu Liu3Qianyu Liu4Qianyu Liu5Zhen Yu6Zhen Yu7Zhen Yu8Chunling Wang9Chunling Wang10Huafu Mai11Huafu Mai12Huafu Mai13Guolan Liu14Guolan Liu15Ruijing Li16Ruijing Li17Gang Pang18Gang Pang19Gang Pang20Dingwu Chen21Dingwu Chen22Huili Liu23Huili Liu24Huili Liu25Jiangyi Yang26Li-Zhen Tao27Li-Zhen Tao28Li-Zhen Tao29State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Sciences and Technology, Guangxi University, Nanning, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, ChinaEukaryotic translation initiation factor 4E1 (eIF4E1) is required for the initiation of protein synthesis. The biological function of eIF4E1 in plant–potyvirus interactions has been extensively studied. However, the role of eIF4E1 in Arabidopsis development remains unclear. In this study, we show that eIF4E1 is highly expressed in the embryo and root apical meristem. In addition, eIF4E1 expression is induced by auxin. eIF4E1 mutants show embryonic cell division defects and short primary roots, a result of reduced cell divisions. Furthermore, our results show that mutation in eIF4E1 severely reduces the accumulation of PIN-FORMED (PIN) proteins and decreases auxin-responsive gene expression at the root tip. Yeast two-hybrid assays identified that eIF4E1 interacts with an RAC/ROP GTPase activator, RopGEF7, which has been previously reported to be involved in the maintenance of the root apical meristem. The interaction between eIF4E1 and RopGEF7 is confirmed by protein pull-down and bimolecular fluorescent complementation assays in plant cells. Taken together, our results demonstrated that eIF4E1 is important for auxin-regulated embryo development and root growth. The eIF4E1–RopGEF7 interaction suggests that eIF4E1 may act through ROP signaling to regulate auxin transport, thus regulating auxin-dependent patterning.https://www.frontiersin.org/articles/10.3389/fpls.2022.938476/fullauxineIF4E1RopGEF7embryo developmentRAC/ROProot growth |
| spellingShingle | Taibo Liu Taibo Liu Taibo Liu Qianyu Liu Qianyu Liu Qianyu Liu Zhen Yu Zhen Yu Zhen Yu Chunling Wang Chunling Wang Huafu Mai Huafu Mai Huafu Mai Guolan Liu Guolan Liu Ruijing Li Ruijing Li Gang Pang Gang Pang Gang Pang Dingwu Chen Dingwu Chen Huili Liu Huili Liu Huili Liu Jiangyi Yang Li-Zhen Tao Li-Zhen Tao Li-Zhen Tao eIF4E1 Regulates Arabidopsis Embryo Development and Root Growth by Interacting With RopGEF7 Frontiers in Plant Science auxin eIF4E1 RopGEF7 embryo development RAC/ROP root growth |
| title | eIF4E1 Regulates Arabidopsis Embryo Development and Root Growth by Interacting With RopGEF7 |
| title_full | eIF4E1 Regulates Arabidopsis Embryo Development and Root Growth by Interacting With RopGEF7 |
| title_fullStr | eIF4E1 Regulates Arabidopsis Embryo Development and Root Growth by Interacting With RopGEF7 |
| title_full_unstemmed | eIF4E1 Regulates Arabidopsis Embryo Development and Root Growth by Interacting With RopGEF7 |
| title_short | eIF4E1 Regulates Arabidopsis Embryo Development and Root Growth by Interacting With RopGEF7 |
| title_sort | eif4e1 regulates arabidopsis embryo development and root growth by interacting with ropgef7 |
| topic | auxin eIF4E1 RopGEF7 embryo development RAC/ROP root growth |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.938476/full |
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