Changing Trends of Antimicrobial Resistance in Neonatal Sepsis: Experience from a Tertiary Care Hospital from West Bengal, India
Introduction: Sepsis is the second leading cause of neonatal mortality in India. Emergence of highly resistant microorganisms as an aetiology of neonatal sepsis is a matter of serious concern. Aim: To study the prevailing aetiological agents in neonatal sepsis and their antimicrobial susceptibi...
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| Language: | English |
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JCDR Research and Publications Private Limited
2021-07-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/15136/49015_CE[Ra1]_F[IK]_PF1(SS_OM)_PFA(SC_KM)_PN(KM).pdf |
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| author | MANJULA DUTTA PIYALI MITRA SHANKHA SUBHRA NAG ABHIJIT DUTTA |
| author_facet | MANJULA DUTTA PIYALI MITRA SHANKHA SUBHRA NAG ABHIJIT DUTTA |
| author_sort | MANJULA DUTTA |
| collection | DOAJ |
| description | Introduction: Sepsis is the second leading cause of neonatal
mortality in India. Emergence of highly resistant microorganisms
as an aetiology of neonatal sepsis is a matter of serious
concern.
Aim: To study the prevailing aetiological agents in neonatal
sepsis and their antimicrobial susceptibility pattern.
Materials and Methods: A prospective observational study
was performed in a tertiary care teaching hospital in neonatal
care units in North Bengal Medical College and Hospital, West
Bengal, India, over a period of two years from March 2017
to February 2019. All the neonates having clinical features
suggestive of sepsis were subjected to blood culture using
BacT/ALERT® PF Plus. Microbial identification and antibiotic
susceptibility testing was done by VITEK-2 automated systems.
Chi-square test was done using Epi info software version 7.1
and p-value <0.05 was considered significant.
Results: Out of 403 neonates investigated for suspected
sepsis, 156 (38.7%) were found to be culture positive. Klebsiella
pneumoniae was the most common organism isolated (n=90,
57.7%) followed by Staphylococcus aureus (n=24, 15.4%) and
Acinetobacter baumannii (n=10, 6.4%). Klebsiella pneumoniae
showed a very high degree of resistance to ampicillin,
amoxycillin-clavulenic acid (100% each), cefotaxime (93.4%),
ceftazidime (92.3%), gentamycin (94.5%) and tobramycin
(94.5%). High resistance of Staphylococcus aureus was seen
against ampicillin (100%), cloxacillin (62.5%), amoxicillinclavulenic acid (70.9%), and cefotaxime (79.2%). Few isolates
of Klebsiella pneumonia (6.6%), Acinetobacter baumannii
(60%) and Enterobacter cloacae (50%) were sensitive only to
colistin and tigecycline.
Conclusion: Most of the isolates showed very high degree
of resistance against first line of antibiotics recommended by
World Health Organisation (WHO) (ampicillin and gentamycin
or amikacin) for empirical treatment of neonatal sepsis.
Emergence of highly resistant organisms sensitive only
to colistin and tigecycline should be considered as an eye
opener. Strict adherence to sepsis prevention along with
regular surveillance of organisms and their sensitivity patterns
is the need of the hour to improve survival by contributing to
antibiotic stewardship. |
| first_indexed | 2024-12-17T22:53:33Z |
| format | Article |
| id | doaj.art-45d91c39cc184b94b83cc1bb9580e90b |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-17T22:53:33Z |
| publishDate | 2021-07-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-45d91c39cc184b94b83cc1bb9580e90b2022-12-21T21:29:36ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2021-07-011507111410.7860/JCDR/2021/49015.15136Changing Trends of Antimicrobial Resistance in Neonatal Sepsis: Experience from a Tertiary Care Hospital from West Bengal, IndiaMANJULA DUTTA0PIYALI MITRA1 SHANKHA SUBHRA NAG2 ABHIJIT DUTTA3Assistant Professor, Department of Microbiology, School of Tropical Medicine, Kolkata, West Bengal, India.Assistant Professor, Department of Microbiology, School of Tropical Medicine, Kolkata, West Bengal, India.Assistant Professor, Department of Microbiology, School of Tropical Medicine, Kolkata, West Bengal, India.Associate Professor, Department of Paediatrics, North Bengal Medical College, Siliguri, West Bengal, IndiaIntroduction: Sepsis is the second leading cause of neonatal mortality in India. Emergence of highly resistant microorganisms as an aetiology of neonatal sepsis is a matter of serious concern. Aim: To study the prevailing aetiological agents in neonatal sepsis and their antimicrobial susceptibility pattern. Materials and Methods: A prospective observational study was performed in a tertiary care teaching hospital in neonatal care units in North Bengal Medical College and Hospital, West Bengal, India, over a period of two years from March 2017 to February 2019. All the neonates having clinical features suggestive of sepsis were subjected to blood culture using BacT/ALERT® PF Plus. Microbial identification and antibiotic susceptibility testing was done by VITEK-2 automated systems. Chi-square test was done using Epi info software version 7.1 and p-value <0.05 was considered significant. Results: Out of 403 neonates investigated for suspected sepsis, 156 (38.7%) were found to be culture positive. Klebsiella pneumoniae was the most common organism isolated (n=90, 57.7%) followed by Staphylococcus aureus (n=24, 15.4%) and Acinetobacter baumannii (n=10, 6.4%). Klebsiella pneumoniae showed a very high degree of resistance to ampicillin, amoxycillin-clavulenic acid (100% each), cefotaxime (93.4%), ceftazidime (92.3%), gentamycin (94.5%) and tobramycin (94.5%). High resistance of Staphylococcus aureus was seen against ampicillin (100%), cloxacillin (62.5%), amoxicillinclavulenic acid (70.9%), and cefotaxime (79.2%). Few isolates of Klebsiella pneumonia (6.6%), Acinetobacter baumannii (60%) and Enterobacter cloacae (50%) were sensitive only to colistin and tigecycline. Conclusion: Most of the isolates showed very high degree of resistance against first line of antibiotics recommended by World Health Organisation (WHO) (ampicillin and gentamycin or amikacin) for empirical treatment of neonatal sepsis. Emergence of highly resistant organisms sensitive only to colistin and tigecycline should be considered as an eye opener. Strict adherence to sepsis prevention along with regular surveillance of organisms and their sensitivity patterns is the need of the hour to improve survival by contributing to antibiotic stewardship.https://jcdr.net/articles/PDF/15136/49015_CE[Ra1]_F[IK]_PF1(SS_OM)_PFA(SC_KM)_PN(KM).pdfacinetobacter baumanniiantimicrobial resistanceklebsiella pneumoniasepsis |
| spellingShingle | MANJULA DUTTA PIYALI MITRA SHANKHA SUBHRA NAG ABHIJIT DUTTA Changing Trends of Antimicrobial Resistance in Neonatal Sepsis: Experience from a Tertiary Care Hospital from West Bengal, India Journal of Clinical and Diagnostic Research acinetobacter baumannii antimicrobial resistance klebsiella pneumonia sepsis |
| title | Changing Trends of Antimicrobial Resistance in Neonatal Sepsis: Experience from a Tertiary Care Hospital from West Bengal, India |
| title_full | Changing Trends of Antimicrobial Resistance in Neonatal Sepsis: Experience from a Tertiary Care Hospital from West Bengal, India |
| title_fullStr | Changing Trends of Antimicrobial Resistance in Neonatal Sepsis: Experience from a Tertiary Care Hospital from West Bengal, India |
| title_full_unstemmed | Changing Trends of Antimicrobial Resistance in Neonatal Sepsis: Experience from a Tertiary Care Hospital from West Bengal, India |
| title_short | Changing Trends of Antimicrobial Resistance in Neonatal Sepsis: Experience from a Tertiary Care Hospital from West Bengal, India |
| title_sort | changing trends of antimicrobial resistance in neonatal sepsis experience from a tertiary care hospital from west bengal india |
| topic | acinetobacter baumannii antimicrobial resistance klebsiella pneumonia sepsis |
| url | https://jcdr.net/articles/PDF/15136/49015_CE[Ra1]_F[IK]_PF1(SS_OM)_PFA(SC_KM)_PN(KM).pdf |
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