Role of <i>MBL2</i> Polymorphisms in Sepsis and Survival: A Pilot Study and In Silico Analysis
Sepsis is a serious infection-induced syndrome with serious ramifications, especially in intensive care units. Global concern motivated the investigation of the role of related genes’ polymorphism in predicting the liability to infection, sepsis, septic shock and survival. Among these genes is the g...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/12/2/460 |
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| author | Mohammed Y. Behairy Ali A. Abdelrahman Hoda Y. Abdallah Emad El-Deen A. Ibrahim Hany R. Hashem Anwar A. Sayed Marwa M. Azab |
| author_facet | Mohammed Y. Behairy Ali A. Abdelrahman Hoda Y. Abdallah Emad El-Deen A. Ibrahim Hany R. Hashem Anwar A. Sayed Marwa M. Azab |
| author_sort | Mohammed Y. Behairy |
| collection | DOAJ |
| description | Sepsis is a serious infection-induced syndrome with serious ramifications, especially in intensive care units. Global concern motivated the investigation of the role of related genes’ polymorphism in predicting the liability to infection, sepsis, septic shock and survival. Among these genes is the gene encoding mannose-binding lectin (MBL), with its remarkable importance in the immune system. However, the previous studies showed conflicting results and ambiguity that urged us to engage with this issue in the Egyptian population. Prediction of functional and structural impacts of single nucleotide polymorphisms (SNPs) was done using in silico methods. A prospective observational study was conducted in intensive care units; one hundred and thirty patients were followed up. Genotyping was performed using real-time polymerase chain reaction (RT-PCR) technology. MBL SNPs showed a remarkable high frequency in our population, as well. No significant association was found between <i>MBL2</i> genotypes and any of our analyses (sepsis, septic shock and survival). Only septic shock and age were independently associated with time of survival by Cox regression analysis. Our study may confirm the redundancy of MBL and the absence of significant impact on sepsis liability and mortality in adult patients. |
| first_indexed | 2024-03-09T22:10:14Z |
| format | Article |
| id | doaj.art-45d920dab05d4dc296cd0a4a3ac1f763 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-09T22:10:14Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-45d920dab05d4dc296cd0a4a3ac1f7632023-11-23T19:32:25ZengMDPI AGDiagnostics2075-44182022-02-0112246010.3390/diagnostics12020460Role of <i>MBL2</i> Polymorphisms in Sepsis and Survival: A Pilot Study and In Silico AnalysisMohammed Y. Behairy0Ali A. Abdelrahman1Hoda Y. Abdallah2Emad El-Deen A. Ibrahim3Hany R. Hashem4Anwar A. Sayed5Marwa M. Azab6Department of Microbiology and Immunology, Faculty of Pharmacy, University of Sadat City, Sadat City 32958, EgyptDepartment of Microbiology and Immunology, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, EgyptMedical Genetics Unit, Department of Histology and Cell Biology, Faculty of Medicine, Suez Canal University, Ismailia 41522, EgyptDepartment of Anesthesia, Intensive Care and Pain Management, Faculty of Medicine, Suez Canal University, Ismailia 41522, EgyptDepartment of Microbiology and Immunology, Faculty of Pharmacy, Fayoum University, Fayoum 63514, EgyptDepartment of Medical Microbiology and Immunology, Taibah University, Madinah 42353, Saudi ArabiaDepartment of Microbiology and Immunology, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, EgyptSepsis is a serious infection-induced syndrome with serious ramifications, especially in intensive care units. Global concern motivated the investigation of the role of related genes’ polymorphism in predicting the liability to infection, sepsis, septic shock and survival. Among these genes is the gene encoding mannose-binding lectin (MBL), with its remarkable importance in the immune system. However, the previous studies showed conflicting results and ambiguity that urged us to engage with this issue in the Egyptian population. Prediction of functional and structural impacts of single nucleotide polymorphisms (SNPs) was done using in silico methods. A prospective observational study was conducted in intensive care units; one hundred and thirty patients were followed up. Genotyping was performed using real-time polymerase chain reaction (RT-PCR) technology. MBL SNPs showed a remarkable high frequency in our population, as well. No significant association was found between <i>MBL2</i> genotypes and any of our analyses (sepsis, septic shock and survival). Only septic shock and age were independently associated with time of survival by Cox regression analysis. Our study may confirm the redundancy of MBL and the absence of significant impact on sepsis liability and mortality in adult patients.https://www.mdpi.com/2075-4418/12/2/460MBLpolymorphisminfectionsepsissurvival |
| spellingShingle | Mohammed Y. Behairy Ali A. Abdelrahman Hoda Y. Abdallah Emad El-Deen A. Ibrahim Hany R. Hashem Anwar A. Sayed Marwa M. Azab Role of <i>MBL2</i> Polymorphisms in Sepsis and Survival: A Pilot Study and In Silico Analysis Diagnostics MBL polymorphism infection sepsis survival |
| title | Role of <i>MBL2</i> Polymorphisms in Sepsis and Survival: A Pilot Study and In Silico Analysis |
| title_full | Role of <i>MBL2</i> Polymorphisms in Sepsis and Survival: A Pilot Study and In Silico Analysis |
| title_fullStr | Role of <i>MBL2</i> Polymorphisms in Sepsis and Survival: A Pilot Study and In Silico Analysis |
| title_full_unstemmed | Role of <i>MBL2</i> Polymorphisms in Sepsis and Survival: A Pilot Study and In Silico Analysis |
| title_short | Role of <i>MBL2</i> Polymorphisms in Sepsis and Survival: A Pilot Study and In Silico Analysis |
| title_sort | role of i mbl2 i polymorphisms in sepsis and survival a pilot study and in silico analysis |
| topic | MBL polymorphism infection sepsis survival |
| url | https://www.mdpi.com/2075-4418/12/2/460 |
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