LncRNA SNHG5 adversely governs follicular growth in PCOS via miR-92a-3p/CDKN1C axis
Summary: Small nucleolar RNA host genes (SNHGs) have been implicated in various biological processes, yet their involvement in polycystic ovary syndrome (PCOS) remains elusive. Specifically, SNHG5, a long non-coding RNA implicated in several human cancers, shows elevated expression in granulosa cell...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-02-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223025993 |
| _version_ | 1797347742148722688 |
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| author | Zuwei Yang Jiexue Pan Chengliang Zhou Chuanjin Yu Zhiyang Zhou Guolian Ding Xinmei Liu Jianzhong Sheng Li Jin Hefeng Huang |
| author_facet | Zuwei Yang Jiexue Pan Chengliang Zhou Chuanjin Yu Zhiyang Zhou Guolian Ding Xinmei Liu Jianzhong Sheng Li Jin Hefeng Huang |
| author_sort | Zuwei Yang |
| collection | DOAJ |
| description | Summary: Small nucleolar RNA host genes (SNHGs) have been implicated in various biological processes, yet their involvement in polycystic ovary syndrome (PCOS) remains elusive. Specifically, SNHG5, a long non-coding RNA implicated in several human cancers, shows elevated expression in granulosa cells (GCs) of PCOS women and induces PCOS-like features when overexpressed in mice. In vitro, SNHG5 inhibits GC proliferation and induces apoptosis and cell-cycle arrest at G0/G1 phase, with RNA-seq indicating its impact on DNA replication and repair pathways. Mechanistically, SNHG5 acts as a competing endogenous RNA by binding to miR-92a-3p, leading to increased expression of target gene CDKN1C, which further suppresses GC proliferation and promotes apoptosis. These findings elucidate the crucial role of SNHG5 in the pathogenesis of PCOS and suggest a potential therapeutic target for this condition. Additional investigations such as large-scale clinical studies and functional assays are warranted to validate and expand upon these findings. |
| first_indexed | 2024-03-08T11:53:10Z |
| format | Article |
| id | doaj.art-45d9b471dfba4177bca68d587d125af3 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-08T11:53:10Z |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-45d9b471dfba4177bca68d587d125af32024-01-24T05:21:42ZengElsevieriScience2589-00422024-02-01272108522LncRNA SNHG5 adversely governs follicular growth in PCOS via miR-92a-3p/CDKN1C axisZuwei Yang0Jiexue Pan1Chengliang Zhou2Chuanjin Yu3Zhiyang Zhou4Guolian Ding5Xinmei Liu6Jianzhong Sheng7Li Jin8Hefeng Huang9Obstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan University, Shanghai, China; The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China; Research Units of Embryo Original Diseases, Chinese Academy of Medical Sciences (No. 2019RU056), Shanghai, ChinaObstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan University, Shanghai, China; Research Units of Embryo Original Diseases, Chinese Academy of Medical Sciences (No. 2019RU056), Shanghai, ChinaThe International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaObstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan University, Shanghai, ChinaObstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan University, Shanghai, ChinaObstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan University, Shanghai, ChinaObstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan University, Shanghai, ChinaObstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan University, Shanghai, ChinaObstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan University, Shanghai, China; Research Units of Embryo Original Diseases, Chinese Academy of Medical Sciences (No. 2019RU056), Shanghai, China; Corresponding authorObstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan University, Shanghai, China; The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China; Research Units of Embryo Original Diseases, Chinese Academy of Medical Sciences (No. 2019RU056), Shanghai, China; Shanghai Key Laboratory of Reproduction and Development, Shanghai, China; Key Laboratory of Reproductive Genetics (Ministry of Education), Department of Reproductive Endocrinology, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, China; Corresponding authorSummary: Small nucleolar RNA host genes (SNHGs) have been implicated in various biological processes, yet their involvement in polycystic ovary syndrome (PCOS) remains elusive. Specifically, SNHG5, a long non-coding RNA implicated in several human cancers, shows elevated expression in granulosa cells (GCs) of PCOS women and induces PCOS-like features when overexpressed in mice. In vitro, SNHG5 inhibits GC proliferation and induces apoptosis and cell-cycle arrest at G0/G1 phase, with RNA-seq indicating its impact on DNA replication and repair pathways. Mechanistically, SNHG5 acts as a competing endogenous RNA by binding to miR-92a-3p, leading to increased expression of target gene CDKN1C, which further suppresses GC proliferation and promotes apoptosis. These findings elucidate the crucial role of SNHG5 in the pathogenesis of PCOS and suggest a potential therapeutic target for this condition. Additional investigations such as large-scale clinical studies and functional assays are warranted to validate and expand upon these findings.http://www.sciencedirect.com/science/article/pii/S2589004223025993Reproductive medicineNatural sciencesBiological sciencesPhysiologyPathophysiology |
| spellingShingle | Zuwei Yang Jiexue Pan Chengliang Zhou Chuanjin Yu Zhiyang Zhou Guolian Ding Xinmei Liu Jianzhong Sheng Li Jin Hefeng Huang LncRNA SNHG5 adversely governs follicular growth in PCOS via miR-92a-3p/CDKN1C axis iScience Reproductive medicine Natural sciences Biological sciences Physiology Pathophysiology |
| title | LncRNA SNHG5 adversely governs follicular growth in PCOS via miR-92a-3p/CDKN1C axis |
| title_full | LncRNA SNHG5 adversely governs follicular growth in PCOS via miR-92a-3p/CDKN1C axis |
| title_fullStr | LncRNA SNHG5 adversely governs follicular growth in PCOS via miR-92a-3p/CDKN1C axis |
| title_full_unstemmed | LncRNA SNHG5 adversely governs follicular growth in PCOS via miR-92a-3p/CDKN1C axis |
| title_short | LncRNA SNHG5 adversely governs follicular growth in PCOS via miR-92a-3p/CDKN1C axis |
| title_sort | lncrna snhg5 adversely governs follicular growth in pcos via mir 92a 3p cdkn1c axis |
| topic | Reproductive medicine Natural sciences Biological sciences Physiology Pathophysiology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223025993 |
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