The cervical transcriptome changes during the menstrual cycle but does not predict the window of implantation
IntroductionThe expression of genes in female reproductive organs is influenced by the cyclic changes in hormone levels during the menstrual cycle. While the molecular changes in the endometrium that facilitate embryo implantation have been extensively studied, there is limited knowledge about the i...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Reproductive Health |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/frph.2023.1224919/full |
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| author | Amruta D. S. Pathare Merli Saare Merli Saare Alvin Meltsov Alvin Meltsov Ankita Lawarde Ankita Lawarde Vijayachitra Modhukur Vijayachitra Modhukur Aive Kalinina Aire Sekavin Viktorija Kukushkina Helle Karro Helle Karro Andres Salumets Andres Salumets Andres Salumets Andres Salumets Maire Peters Maire Peters |
| author_facet | Amruta D. S. Pathare Merli Saare Merli Saare Alvin Meltsov Alvin Meltsov Ankita Lawarde Ankita Lawarde Vijayachitra Modhukur Vijayachitra Modhukur Aive Kalinina Aire Sekavin Viktorija Kukushkina Helle Karro Helle Karro Andres Salumets Andres Salumets Andres Salumets Andres Salumets Maire Peters Maire Peters |
| author_sort | Amruta D. S. Pathare |
| collection | DOAJ |
| description | IntroductionThe expression of genes in female reproductive organs is influenced by the cyclic changes in hormone levels during the menstrual cycle. While the molecular changes in the endometrium that facilitate embryo implantation have been extensively studied, there is limited knowledge about the impact of the menstrual cycle on cervical cells. Cervical cells can be easily and routinely collected using a cytobrush during gynecological examination, offering a standardized approach for diagnostic testing. In this study we investigated how the transcriptome of cervical cells changes during the menstrual cycle and assessed the utility of these cells to determine endometrial receptivity.MethodsEndocervical cells were collected with cytobrushes from 16 healthy women at different menstrual cycle phases in natural cycles and from four women undergoing hormonal replacement cycles. RNA sequencing was applied to gain insight into the transcriptome of cervical cells.ResultsTranscriptome analysis identified four differentially expressed genes (DEGs) between early- and mid-secretory samples, suggesting that the transcriptome of cervical cells does not change significantly during the opening of the implantation window. The most differences appeared during the transition to the late secretory phase (2136 DEGs) before the onset of menstruation. Cervical cells collected during hormonal replacement cycles showed 1899 DEGs enriched in immune system processes.ConclusionsThe results of our study suggested that cervical cells undergo moderate transcriptomic changes throughout the menstrual cycle; however, these changes do not reflect the gene expression pattern of endometrial tissue and offer little or no potential for endometrial receptivity diagnostics. |
| first_indexed | 2024-03-12T23:39:33Z |
| format | Article |
| id | doaj.art-45dc6171cefb41d5bd7f408aa4ef3e44 |
| institution | Directory Open Access Journal |
| issn | 2673-3153 |
| language | English |
| last_indexed | 2024-03-12T23:39:33Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Reproductive Health |
| spelling | doaj.art-45dc6171cefb41d5bd7f408aa4ef3e442023-07-14T23:07:02ZengFrontiers Media S.A.Frontiers in Reproductive Health2673-31532023-07-01510.3389/frph.2023.12249191224919The cervical transcriptome changes during the menstrual cycle but does not predict the window of implantationAmruta D. S. Pathare0Merli Saare1Merli Saare2Alvin Meltsov3Alvin Meltsov4Ankita Lawarde5Ankita Lawarde6Vijayachitra Modhukur7Vijayachitra Modhukur8Aive Kalinina9Aire Sekavin10Viktorija Kukushkina11Helle Karro12Helle Karro13Andres Salumets14Andres Salumets15Andres Salumets16Andres Salumets17Maire Peters18Maire Peters19Department of Obstetrics and Gynecology, Institute of Clinical Medicine, University of Tartu, Tartu, EstoniaDepartment of Obstetrics and Gynecology, Institute of Clinical Medicine, University of Tartu, Tartu, EstoniaCompetence Centre on Health Technologies, Tartu, EstoniaCompetence Centre on Health Technologies, Tartu, EstoniaDepartment of Genetics and Cell Biology, GROW School for Oncology and Developmental Biology, Maastricht University, Maastricht, NetherlandsCompetence Centre on Health Technologies, Tartu, EstoniaInstitute of Clinical Medicine, University of Tartu, Tartu, EstoniaDepartment of Obstetrics and Gynecology, Institute of Clinical Medicine, University of Tartu, Tartu, EstoniaCompetence Centre on Health Technologies, Tartu, EstoniaSouth Estonia Hospital, Võru, EstoniaWomen’s Clinic, Tartu University Hospital, Tartu, EstoniaInstitute of Genomics, University of Tartu, Tartu, EstoniaDepartment of Obstetrics and Gynecology, Institute of Clinical Medicine, University of Tartu, Tartu, EstoniaWomen’s Clinic, Tartu University Hospital, Tartu, EstoniaDepartment of Obstetrics and Gynecology, Institute of Clinical Medicine, University of Tartu, Tartu, EstoniaCompetence Centre on Health Technologies, Tartu, EstoniaInstitute of Genomics, University of Tartu, Tartu, EstoniaDivision of Obstetrics and Gynecology, Department of Clinical Science, Intervention and Technology (CLINTEC), Karolinska Institutet, and Karolinska University Hospital, Stockholm, SwedenDepartment of Obstetrics and Gynecology, Institute of Clinical Medicine, University of Tartu, Tartu, EstoniaCompetence Centre on Health Technologies, Tartu, EstoniaIntroductionThe expression of genes in female reproductive organs is influenced by the cyclic changes in hormone levels during the menstrual cycle. While the molecular changes in the endometrium that facilitate embryo implantation have been extensively studied, there is limited knowledge about the impact of the menstrual cycle on cervical cells. Cervical cells can be easily and routinely collected using a cytobrush during gynecological examination, offering a standardized approach for diagnostic testing. In this study we investigated how the transcriptome of cervical cells changes during the menstrual cycle and assessed the utility of these cells to determine endometrial receptivity.MethodsEndocervical cells were collected with cytobrushes from 16 healthy women at different menstrual cycle phases in natural cycles and from four women undergoing hormonal replacement cycles. RNA sequencing was applied to gain insight into the transcriptome of cervical cells.ResultsTranscriptome analysis identified four differentially expressed genes (DEGs) between early- and mid-secretory samples, suggesting that the transcriptome of cervical cells does not change significantly during the opening of the implantation window. The most differences appeared during the transition to the late secretory phase (2136 DEGs) before the onset of menstruation. Cervical cells collected during hormonal replacement cycles showed 1899 DEGs enriched in immune system processes.ConclusionsThe results of our study suggested that cervical cells undergo moderate transcriptomic changes throughout the menstrual cycle; however, these changes do not reflect the gene expression pattern of endometrial tissue and offer little or no potential for endometrial receptivity diagnostics.https://www.frontiersin.org/articles/10.3389/frph.2023.1224919/fullcervix uteriendometriummenstrual cyclemolecular diagnostic techniquestranscriptome |
| spellingShingle | Amruta D. S. Pathare Merli Saare Merli Saare Alvin Meltsov Alvin Meltsov Ankita Lawarde Ankita Lawarde Vijayachitra Modhukur Vijayachitra Modhukur Aive Kalinina Aire Sekavin Viktorija Kukushkina Helle Karro Helle Karro Andres Salumets Andres Salumets Andres Salumets Andres Salumets Maire Peters Maire Peters The cervical transcriptome changes during the menstrual cycle but does not predict the window of implantation Frontiers in Reproductive Health cervix uteri endometrium menstrual cycle molecular diagnostic techniques transcriptome |
| title | The cervical transcriptome changes during the menstrual cycle but does not predict the window of implantation |
| title_full | The cervical transcriptome changes during the menstrual cycle but does not predict the window of implantation |
| title_fullStr | The cervical transcriptome changes during the menstrual cycle but does not predict the window of implantation |
| title_full_unstemmed | The cervical transcriptome changes during the menstrual cycle but does not predict the window of implantation |
| title_short | The cervical transcriptome changes during the menstrual cycle but does not predict the window of implantation |
| title_sort | cervical transcriptome changes during the menstrual cycle but does not predict the window of implantation |
| topic | cervix uteri endometrium menstrual cycle molecular diagnostic techniques transcriptome |
| url | https://www.frontiersin.org/articles/10.3389/frph.2023.1224919/full |
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