Random Transfer of <i>Ogataea polymorpha</i> Genes into <i>Saccharomyces cerevisiae</i> Reveals a Complex Background of Heat Tolerance
Horizontal gene transfer, a process through which an organism acquires genes from other organisms, is a rare evolutionary event in yeasts. Artificial random gene transfer can emerge as a valuable tool in yeast bioengineering to investigate the background of complex phenotypes, such as heat tolerance...
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MDPI AG
2021-04-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/7/4/302 |
| _version_ | 1797537580223299584 |
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| author | Taisuke Seike Yuki Narazaki Yoshinobu Kaneko Hiroshi Shimizu Fumio Matsuda |
| author_facet | Taisuke Seike Yuki Narazaki Yoshinobu Kaneko Hiroshi Shimizu Fumio Matsuda |
| author_sort | Taisuke Seike |
| collection | DOAJ |
| description | Horizontal gene transfer, a process through which an organism acquires genes from other organisms, is a rare evolutionary event in yeasts. Artificial random gene transfer can emerge as a valuable tool in yeast bioengineering to investigate the background of complex phenotypes, such as heat tolerance. In this study, a cDNA library was constructed from the mRNA of a methylotrophic yeast, <i>Ogataea polymorpha</i>, and then introduced into <i>Saccharomyces cerevisiae</i>. <i>Ogataea polymorpha</i> was selected because it is one of the most heat-tolerant species among yeasts. Screening of <i>S. cerevisiae</i> populations expressing <i>O. polymorpha</i> genes at high temperatures identified 59 <i>O. polymorpha</i> genes that contribute to heat tolerance. Gene enrichment analysis indicated that certain <i>S. cerevisiae</i> functions, including protein synthesis, were highly temperature-sensitive. Additionally, the results confirmed that heat tolerance in yeast is a complex phenotype dependent on multiple quantitative loci. Random gene transfer would be a useful tool for future bioengineering studies on yeasts. |
| first_indexed | 2024-03-10T12:17:12Z |
| format | Article |
| id | doaj.art-45e3a50d6daf4ecc8ed84b925ab492c4 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-10T12:17:12Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-45e3a50d6daf4ecc8ed84b925ab492c42023-11-21T15:44:02ZengMDPI AGJournal of Fungi2309-608X2021-04-017430210.3390/jof7040302Random Transfer of <i>Ogataea polymorpha</i> Genes into <i>Saccharomyces cerevisiae</i> Reveals a Complex Background of Heat ToleranceTaisuke Seike0Yuki Narazaki1Yoshinobu Kaneko2Hiroshi Shimizu3Fumio Matsuda4Department of Bioinformatics Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka 565-0871, JapanDepartment of Bioinformatics Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka 565-0871, JapanYeast Genetic Resources Laboratory, Graduate School of Engineering, Osaka University, Osaka 565-0871, JapanDepartment of Bioinformatics Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka 565-0871, JapanDepartment of Bioinformatics Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka 565-0871, JapanHorizontal gene transfer, a process through which an organism acquires genes from other organisms, is a rare evolutionary event in yeasts. Artificial random gene transfer can emerge as a valuable tool in yeast bioengineering to investigate the background of complex phenotypes, such as heat tolerance. In this study, a cDNA library was constructed from the mRNA of a methylotrophic yeast, <i>Ogataea polymorpha</i>, and then introduced into <i>Saccharomyces cerevisiae</i>. <i>Ogataea polymorpha</i> was selected because it is one of the most heat-tolerant species among yeasts. Screening of <i>S. cerevisiae</i> populations expressing <i>O. polymorpha</i> genes at high temperatures identified 59 <i>O. polymorpha</i> genes that contribute to heat tolerance. Gene enrichment analysis indicated that certain <i>S. cerevisiae</i> functions, including protein synthesis, were highly temperature-sensitive. Additionally, the results confirmed that heat tolerance in yeast is a complex phenotype dependent on multiple quantitative loci. Random gene transfer would be a useful tool for future bioengineering studies on yeasts.https://www.mdpi.com/2309-608X/7/4/302<i>Saccharomyces cerevisiae</i>cDNA libraryheat tolerancerandom gene transfer<i>Ogataea polymorpha</i> |
| spellingShingle | Taisuke Seike Yuki Narazaki Yoshinobu Kaneko Hiroshi Shimizu Fumio Matsuda Random Transfer of <i>Ogataea polymorpha</i> Genes into <i>Saccharomyces cerevisiae</i> Reveals a Complex Background of Heat Tolerance Journal of Fungi <i>Saccharomyces cerevisiae</i> cDNA library heat tolerance random gene transfer <i>Ogataea polymorpha</i> |
| title | Random Transfer of <i>Ogataea polymorpha</i> Genes into <i>Saccharomyces cerevisiae</i> Reveals a Complex Background of Heat Tolerance |
| title_full | Random Transfer of <i>Ogataea polymorpha</i> Genes into <i>Saccharomyces cerevisiae</i> Reveals a Complex Background of Heat Tolerance |
| title_fullStr | Random Transfer of <i>Ogataea polymorpha</i> Genes into <i>Saccharomyces cerevisiae</i> Reveals a Complex Background of Heat Tolerance |
| title_full_unstemmed | Random Transfer of <i>Ogataea polymorpha</i> Genes into <i>Saccharomyces cerevisiae</i> Reveals a Complex Background of Heat Tolerance |
| title_short | Random Transfer of <i>Ogataea polymorpha</i> Genes into <i>Saccharomyces cerevisiae</i> Reveals a Complex Background of Heat Tolerance |
| title_sort | random transfer of i ogataea polymorpha i genes into i saccharomyces cerevisiae i reveals a complex background of heat tolerance |
| topic | <i>Saccharomyces cerevisiae</i> cDNA library heat tolerance random gene transfer <i>Ogataea polymorpha</i> |
| url | https://www.mdpi.com/2309-608X/7/4/302 |
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