Hydrolysis of whey lactose by immobilized β-Galactosidase
Hydrolysis of whey lactose to glucose and galactose by immobilized galactosidase comes as an alternative to enlarge the possibilities of commercial use of this feedstock. To be applied at industrial scale, the process should be performed continuously .This work aimed to study the hydrolysis of whey...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2008-12-01
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| Series: | Brazilian Archives of Biology and Technology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600019 |
| _version_ | 1819054685412130816 |
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| author | Marcela Panaro Mariotti Hideko Yamanaka Angela Regina Araujo Henrique Celso Trevisan |
| author_facet | Marcela Panaro Mariotti Hideko Yamanaka Angela Regina Araujo Henrique Celso Trevisan |
| author_sort | Marcela Panaro Mariotti |
| collection | DOAJ |
| description | Hydrolysis of whey lactose to glucose and galactose by immobilized galactosidase comes as an alternative to enlarge the possibilities of commercial use of this feedstock. To be applied at industrial scale, the process should be performed continuously .This work aimed to study the hydrolysis of whey lactose by an immobilized enzyme reactor. b-Galactosidase from Aspergillus oryzae was immobilized on silica and activity and stability were evaluated. The best immobilization results were attained by using glutaraldehyde as support's activator and enzyme stabilizer. The optimized enzyme proportion for immobilization was 15-20 mg g-1 of support. Treatments of whey were performed (microfiltration, thermal treatment and ultrafiltration), seeking the elimination of sludge, and the effects on operating the fixed bed reactor were evaluated. Ultrafiltration was the best treatment towards a proper substrate solution for feeding the reactor.<br>A hidrólise de lactose de soro de leite, resultando em glicose e galactose, apresenta-se como uma alternativa para ampliar as possibilidades de uso comercial deste insumo. Para ser aplicado em escala industrial, o processo deve ser operado de modo contínuo. Reporta-se o estudo de um sistema objetivando hidrólise de lactose de soro de leite através de um reator com enzima imobilizada. b-Galactosidase de Aspergillus oryzae foi imobilizada em sílica, sendo avaliadas a estabilidade e atividade. Os melhores resultados de imobilização foram obtidos usando glutaraldeído como ativante do suporte e estabilizador da enzima. A proporção otimizada entre enzima e suporte foi 15-20 mg.g-1. Foram estudadas formas de tratamento do soro (microfiltração, tratamento térmico e ultrafiltração), objetivando eliminação de material suspenso, e avaliando os efeitos na operação de reator de leito fixo. A ultrafiltração foi o melhor tratamento, na busca de uma solução de substrato apropriada para o reator contínuo. |
| first_indexed | 2024-12-21T12:55:33Z |
| format | Article |
| id | doaj.art-45e6caef5fdb47e6babb268f117f90e0 |
| institution | Directory Open Access Journal |
| issn | 1516-8913 1678-4324 |
| language | English |
| last_indexed | 2024-12-21T12:55:33Z |
| publishDate | 2008-12-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-45e6caef5fdb47e6babb268f117f90e02022-12-21T19:03:21ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242008-12-015161233124010.1590/S1516-89132008000600019Hydrolysis of whey lactose by immobilized β-GalactosidaseMarcela Panaro MariottiHideko YamanakaAngela Regina AraujoHenrique Celso TrevisanHydrolysis of whey lactose to glucose and galactose by immobilized galactosidase comes as an alternative to enlarge the possibilities of commercial use of this feedstock. To be applied at industrial scale, the process should be performed continuously .This work aimed to study the hydrolysis of whey lactose by an immobilized enzyme reactor. b-Galactosidase from Aspergillus oryzae was immobilized on silica and activity and stability were evaluated. The best immobilization results were attained by using glutaraldehyde as support's activator and enzyme stabilizer. The optimized enzyme proportion for immobilization was 15-20 mg g-1 of support. Treatments of whey were performed (microfiltration, thermal treatment and ultrafiltration), seeking the elimination of sludge, and the effects on operating the fixed bed reactor were evaluated. Ultrafiltration was the best treatment towards a proper substrate solution for feeding the reactor.<br>A hidrólise de lactose de soro de leite, resultando em glicose e galactose, apresenta-se como uma alternativa para ampliar as possibilidades de uso comercial deste insumo. Para ser aplicado em escala industrial, o processo deve ser operado de modo contínuo. Reporta-se o estudo de um sistema objetivando hidrólise de lactose de soro de leite através de um reator com enzima imobilizada. b-Galactosidase de Aspergillus oryzae foi imobilizada em sílica, sendo avaliadas a estabilidade e atividade. Os melhores resultados de imobilização foram obtidos usando glutaraldeído como ativante do suporte e estabilizador da enzima. A proporção otimizada entre enzima e suporte foi 15-20 mg.g-1. Foram estudadas formas de tratamento do soro (microfiltração, tratamento térmico e ultrafiltração), objetivando eliminação de material suspenso, e avaliando os efeitos na operação de reator de leito fixo. A ultrafiltração foi o melhor tratamento, na busca de uma solução de substrato apropriada para o reator contínuo.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600019β-galactosidaseimmobilizationlactasewheylactosehydrolysis |
| spellingShingle | Marcela Panaro Mariotti Hideko Yamanaka Angela Regina Araujo Henrique Celso Trevisan Hydrolysis of whey lactose by immobilized β-Galactosidase Brazilian Archives of Biology and Technology β-galactosidase immobilization lactase whey lactose hydrolysis |
| title | Hydrolysis of whey lactose by immobilized β-Galactosidase |
| title_full | Hydrolysis of whey lactose by immobilized β-Galactosidase |
| title_fullStr | Hydrolysis of whey lactose by immobilized β-Galactosidase |
| title_full_unstemmed | Hydrolysis of whey lactose by immobilized β-Galactosidase |
| title_short | Hydrolysis of whey lactose by immobilized β-Galactosidase |
| title_sort | hydrolysis of whey lactose by immobilized 946 galactosidase |
| topic | β-galactosidase immobilization lactase whey lactose hydrolysis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600019 |
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