Cytotoxicity of green-synthesized silver nanoparticles by Adansonia digitata fruit extract against HTC116 and SW480 human colon cancer cell lines
Nanoparticles synthesized from plants are being explored for cancer treatment therapies all over the world. This study reported the eco-friendly and low-cost method for the green synthesis of silver nanoparticles (AgNPs) from Adansonia digitata fruit as a reducing and capping agent. The anti-cancer...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2022-04-01
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| Series: | Green Processing and Synthesis |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/gps-2022-0031 |
| _version_ | 1811236624860708864 |
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| author | Almukaynizi Fatimah Basil Daghestani Maha H. Awad Manal A. Althomali Arwa Merghani Nada M. Bukhari Wadha I. Algahtani Norah M. Al-Zuhairy Shatha S. ALOthman Ahlam M. Alsenani Eman A. Alojayan Badrih O. Al-Saif Khulud S. Bhat Ramesa Shafi |
| author_facet | Almukaynizi Fatimah Basil Daghestani Maha H. Awad Manal A. Althomali Arwa Merghani Nada M. Bukhari Wadha I. Algahtani Norah M. Al-Zuhairy Shatha S. ALOthman Ahlam M. Alsenani Eman A. Alojayan Badrih O. Al-Saif Khulud S. Bhat Ramesa Shafi |
| author_sort | Almukaynizi Fatimah Basil |
| collection | DOAJ |
| description | Nanoparticles synthesized from plants are being explored for cancer treatment therapies all over the world. This study reported the eco-friendly and low-cost method for the green synthesis of silver nanoparticles (AgNPs) from Adansonia digitata fruit as a reducing and capping agent. The anti-cancer potential of synthesized particles was explored against HTC116 and SW480 colon cancer cell lines. Prepared AgNPs were characterized by ultraviolet-visible spectroscopy, zeta potential, transmission electronic microscopy, scanning electronic microscopy, Fourier transform infrared, and energy-dispersive spectrum. The cytotoxicity was determined with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and expression levels of four genes (CTNNB1, APC, LRP5, and LRP6) were checked by reverse transcription polymerase chain reaction. The sharp peak of surface plasmon resonance at 400 nm confirms the formation of AgNPs. Dynamic light scattering showed average sizes of 16.34 nm with a polydispersity index of 0.193. A. digitata AgNPs were spherical with slight aggregated. AgNPs were more cytotoxic than A. digitata extract and decrease the expression of CTNNB1 and LRP6 genes while LRP5 gene expression was increased in both cell lines. APC gene expression was decreased in SW480 but increased in HTC116 with treatment. Overall, this study suggested that AgNPs synthesized by A. digitata fruit extract can be an attractive candidate for anticancer applications. |
| first_indexed | 2024-04-12T12:11:09Z |
| format | Article |
| id | doaj.art-45e90dd5ae4c41469677f7ee4220d76d |
| institution | Directory Open Access Journal |
| issn | 2191-9550 |
| language | English |
| last_indexed | 2024-04-12T12:11:09Z |
| publishDate | 2022-04-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Green Processing and Synthesis |
| spelling | doaj.art-45e90dd5ae4c41469677f7ee4220d76d2022-12-22T03:33:34ZengDe GruyterGreen Processing and Synthesis2191-95502022-04-0111141142210.1515/gps-2022-0031Cytotoxicity of green-synthesized silver nanoparticles by Adansonia digitata fruit extract against HTC116 and SW480 human colon cancer cell linesAlmukaynizi Fatimah Basil0Daghestani Maha H.1Awad Manal A.2Althomali Arwa3Merghani Nada M.4Bukhari Wadha I.5Algahtani Norah M.6Al-Zuhairy Shatha S.7ALOthman Ahlam M.8Alsenani Eman A.9Alojayan Badrih O.10Al-Saif Khulud S.11Bhat Ramesa Shafi12Prince Naif Bin AbdulAziz Health Research Center, King Saud University, Riyadh, Saudi ArabiaDepartment of Zoology, College of Science, King Saud University, Riyadh, Saudi ArabiaKing Abdullah Institute for Nanotechnology, King Saud University, Riyadh, Saudi ArabiaPrince Naif Bin AbdulAziz Health Research Center, King Saud University, Riyadh, Saudi ArabiaCentral Laboratory, King Saud University, Riyadh, Saudi ArabiaCentral Laboratory, King Saud University, Riyadh, Saudi ArabiaCentral Laboratory, King Saud University, Riyadh, Saudi ArabiaCentral Laboratory, King Saud University, Riyadh, Saudi ArabiaCentral Laboratory, King Saud University, Riyadh, Saudi ArabiaCentral Laboratory, King Saud University, Riyadh, Saudi ArabiaCentral Laboratory, King Saud University, Riyadh, Saudi ArabiaCentral Laboratory, King Saud University, Riyadh, Saudi ArabiaBiochemistry Department, College of Science, King Saud University, PO Box 22452, 11495, Riyadh, Saudi ArabiaNanoparticles synthesized from plants are being explored for cancer treatment therapies all over the world. This study reported the eco-friendly and low-cost method for the green synthesis of silver nanoparticles (AgNPs) from Adansonia digitata fruit as a reducing and capping agent. The anti-cancer potential of synthesized particles was explored against HTC116 and SW480 colon cancer cell lines. Prepared AgNPs were characterized by ultraviolet-visible spectroscopy, zeta potential, transmission electronic microscopy, scanning electronic microscopy, Fourier transform infrared, and energy-dispersive spectrum. The cytotoxicity was determined with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and expression levels of four genes (CTNNB1, APC, LRP5, and LRP6) were checked by reverse transcription polymerase chain reaction. The sharp peak of surface plasmon resonance at 400 nm confirms the formation of AgNPs. Dynamic light scattering showed average sizes of 16.34 nm with a polydispersity index of 0.193. A. digitata AgNPs were spherical with slight aggregated. AgNPs were more cytotoxic than A. digitata extract and decrease the expression of CTNNB1 and LRP6 genes while LRP5 gene expression was increased in both cell lines. APC gene expression was decreased in SW480 but increased in HTC116 with treatment. Overall, this study suggested that AgNPs synthesized by A. digitata fruit extract can be an attractive candidate for anticancer applications.https://doi.org/10.1515/gps-2022-0031adansonia digitatasilver nanoparticlesgene expressionhuman colon cancer cell line |
| spellingShingle | Almukaynizi Fatimah Basil Daghestani Maha H. Awad Manal A. Althomali Arwa Merghani Nada M. Bukhari Wadha I. Algahtani Norah M. Al-Zuhairy Shatha S. ALOthman Ahlam M. Alsenani Eman A. Alojayan Badrih O. Al-Saif Khulud S. Bhat Ramesa Shafi Cytotoxicity of green-synthesized silver nanoparticles by Adansonia digitata fruit extract against HTC116 and SW480 human colon cancer cell lines Green Processing and Synthesis adansonia digitata silver nanoparticles gene expression human colon cancer cell line |
| title | Cytotoxicity of green-synthesized silver nanoparticles by Adansonia digitata fruit extract against HTC116 and SW480 human colon cancer cell lines |
| title_full | Cytotoxicity of green-synthesized silver nanoparticles by Adansonia digitata fruit extract against HTC116 and SW480 human colon cancer cell lines |
| title_fullStr | Cytotoxicity of green-synthesized silver nanoparticles by Adansonia digitata fruit extract against HTC116 and SW480 human colon cancer cell lines |
| title_full_unstemmed | Cytotoxicity of green-synthesized silver nanoparticles by Adansonia digitata fruit extract against HTC116 and SW480 human colon cancer cell lines |
| title_short | Cytotoxicity of green-synthesized silver nanoparticles by Adansonia digitata fruit extract against HTC116 and SW480 human colon cancer cell lines |
| title_sort | cytotoxicity of green synthesized silver nanoparticles by adansonia digitata fruit extract against htc116 and sw480 human colon cancer cell lines |
| topic | adansonia digitata silver nanoparticles gene expression human colon cancer cell line |
| url | https://doi.org/10.1515/gps-2022-0031 |
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