Drug Inducible CRISPR/Cas Systems
Clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) systems have been employed as a powerful versatile technology for programmable gene editing, transcriptional modulation, epigenetic modulation, and genome labeling, etc. Yet better control of their a...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-01-01
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| Series: | Computational and Structural Biotechnology Journal |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2001037018303568 |
| _version_ | 1818923518626103296 |
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| author | Jingfang Zhang Li Chen Ju Zhang Yu Wang |
| author_facet | Jingfang Zhang Li Chen Ju Zhang Yu Wang |
| author_sort | Jingfang Zhang |
| collection | DOAJ |
| description | Clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) systems have been employed as a powerful versatile technology for programmable gene editing, transcriptional modulation, epigenetic modulation, and genome labeling, etc. Yet better control of their activity is important to accomplish greater precision and to reduce undesired outcomes such as off-target events. The use of small molecules to control CRISPR/Cas activity represents a promising direction. Here, we provide an updated review on multiple drug inducible CRISPR/Cas systems and discuss their distinct properties. We arbitrarily divided the emerging drug inducible CRISPR/Cas systems into two categories based on whether at transcription or protein level does chemical control occurs. The first category includes Tet-On/Off system and Cre-dependent system. The second category includes chemically induced proximity systems, intein splicing system, 4-Hydroxytamoxifen-Estrogen Receptor based nuclear localization systems, allosterically regulated Cas9 system, and destabilizing domain mediated protein degradation systems. Finally, the advantages and limitations of each system were summarized. |
| first_indexed | 2024-12-20T02:10:43Z |
| format | Article |
| id | doaj.art-45e92268293b43ea80b98f472ece0a82 |
| institution | Directory Open Access Journal |
| issn | 2001-0370 |
| language | English |
| last_indexed | 2024-12-20T02:10:43Z |
| publishDate | 2019-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Computational and Structural Biotechnology Journal |
| spelling | doaj.art-45e92268293b43ea80b98f472ece0a822022-12-21T19:57:03ZengElsevierComputational and Structural Biotechnology Journal2001-03702019-01-011711711177Drug Inducible CRISPR/Cas SystemsJingfang Zhang0Li Chen1Ju Zhang2Yu Wang3School of Life Sciences, Beijing University of Chinese Medicine, Beijing 100029, China; Corresponding authors.State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China; Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China; Corresponding authors.Clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) systems have been employed as a powerful versatile technology for programmable gene editing, transcriptional modulation, epigenetic modulation, and genome labeling, etc. Yet better control of their activity is important to accomplish greater precision and to reduce undesired outcomes such as off-target events. The use of small molecules to control CRISPR/Cas activity represents a promising direction. Here, we provide an updated review on multiple drug inducible CRISPR/Cas systems and discuss their distinct properties. We arbitrarily divided the emerging drug inducible CRISPR/Cas systems into two categories based on whether at transcription or protein level does chemical control occurs. The first category includes Tet-On/Off system and Cre-dependent system. The second category includes chemically induced proximity systems, intein splicing system, 4-Hydroxytamoxifen-Estrogen Receptor based nuclear localization systems, allosterically regulated Cas9 system, and destabilizing domain mediated protein degradation systems. Finally, the advantages and limitations of each system were summarized.http://www.sciencedirect.com/science/article/pii/S2001037018303568 |
| spellingShingle | Jingfang Zhang Li Chen Ju Zhang Yu Wang Drug Inducible CRISPR/Cas Systems Computational and Structural Biotechnology Journal |
| title | Drug Inducible CRISPR/Cas Systems |
| title_full | Drug Inducible CRISPR/Cas Systems |
| title_fullStr | Drug Inducible CRISPR/Cas Systems |
| title_full_unstemmed | Drug Inducible CRISPR/Cas Systems |
| title_short | Drug Inducible CRISPR/Cas Systems |
| title_sort | drug inducible crispr cas systems |
| url | http://www.sciencedirect.com/science/article/pii/S2001037018303568 |
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