Assessing the Biological Safety of Atmospheric Cold Plasma Treated Wheat Using Cell and Insect Models
Atmospheric cold plasma (ACP) is under investigation for an extensive range of biocontrol applications in food biosystems. However, the development of a novel intervention technology requires a thorough evaluation of the potential for negative effects and the implications for the human and animal fo...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/9/7/898 |
| _version_ | 1827713470889459712 |
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| author | Agata Los Dana Ziuzina Robin Van Cleynenbreugel Daniela Boehm Paula Bourke |
| author_facet | Agata Los Dana Ziuzina Robin Van Cleynenbreugel Daniela Boehm Paula Bourke |
| author_sort | Agata Los |
| collection | DOAJ |
| description | Atmospheric cold plasma (ACP) is under investigation for an extensive range of biocontrol applications in food biosystems. However, the development of a novel intervention technology requires a thorough evaluation of the potential for negative effects and the implications for the human and animal food chains’ safety. The evaluations were performed using a contained, high-voltage, dielectric barrier discharge plasma system. The cytotoxicity of two types of food models—a liquid model (wheat model medium (WMM)) vs. a solid model (wheat grain extract (WGE)) was compared in vitro using the mammalian cell line CHO-K1. The residual toxicity of ACP treatment of grains for food purposes was assessed using the invertebrate model <i>Tribolium castaneum</i>, by feeding the beetles with flour produced from ACP-treated wheat grains. The cytotoxic effects and changes in the chemistry of the ACP-treated samples were more pronounced in samples treated in a liquid form as opposed to actual wheat grains. The feeding trial using <i>T. castaneum</i> demonstrated no negative impacts on the survivability or weight profiles of insects. Investigations into the interactions of plasma-generated species with secondary metabolites in the food matrices are necessary to ensure the safety of plasma for food applications. |
| first_indexed | 2024-03-10T18:37:21Z |
| format | Article |
| id | doaj.art-45e935a9e4004499a6f4400693168603 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T18:37:21Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-45e935a9e4004499a6f44006931686032023-11-20T06:08:25ZengMDPI AGFoods2304-81582020-07-019789810.3390/foods9070898Assessing the Biological Safety of Atmospheric Cold Plasma Treated Wheat Using Cell and Insect ModelsAgata Los0Dana Ziuzina1Robin Van Cleynenbreugel2Daniela Boehm3Paula Bourke4Environmental Sustainability and Health Institute, Technological University Dublin, Dublin 7, IrelandEnvironmental Sustainability and Health Institute, Technological University Dublin, Dublin 7, IrelandEnvironmental Sustainability and Health Institute, Technological University Dublin, Dublin 7, IrelandEnvironmental Sustainability and Health Institute, Technological University Dublin, Dublin 7, IrelandEnvironmental Sustainability and Health Institute, Technological University Dublin, Dublin 7, IrelandAtmospheric cold plasma (ACP) is under investigation for an extensive range of biocontrol applications in food biosystems. However, the development of a novel intervention technology requires a thorough evaluation of the potential for negative effects and the implications for the human and animal food chains’ safety. The evaluations were performed using a contained, high-voltage, dielectric barrier discharge plasma system. The cytotoxicity of two types of food models—a liquid model (wheat model medium (WMM)) vs. a solid model (wheat grain extract (WGE)) was compared in vitro using the mammalian cell line CHO-K1. The residual toxicity of ACP treatment of grains for food purposes was assessed using the invertebrate model <i>Tribolium castaneum</i>, by feeding the beetles with flour produced from ACP-treated wheat grains. The cytotoxic effects and changes in the chemistry of the ACP-treated samples were more pronounced in samples treated in a liquid form as opposed to actual wheat grains. The feeding trial using <i>T. castaneum</i> demonstrated no negative impacts on the survivability or weight profiles of insects. Investigations into the interactions of plasma-generated species with secondary metabolites in the food matrices are necessary to ensure the safety of plasma for food applications.https://www.mdpi.com/2304-8158/9/7/898atmospheric cold plasmafood processingwheat grainssafety evaluationsfood model systemscytotoxicity |
| spellingShingle | Agata Los Dana Ziuzina Robin Van Cleynenbreugel Daniela Boehm Paula Bourke Assessing the Biological Safety of Atmospheric Cold Plasma Treated Wheat Using Cell and Insect Models Foods atmospheric cold plasma food processing wheat grains safety evaluations food model systems cytotoxicity |
| title | Assessing the Biological Safety of Atmospheric Cold Plasma Treated Wheat Using Cell and Insect Models |
| title_full | Assessing the Biological Safety of Atmospheric Cold Plasma Treated Wheat Using Cell and Insect Models |
| title_fullStr | Assessing the Biological Safety of Atmospheric Cold Plasma Treated Wheat Using Cell and Insect Models |
| title_full_unstemmed | Assessing the Biological Safety of Atmospheric Cold Plasma Treated Wheat Using Cell and Insect Models |
| title_short | Assessing the Biological Safety of Atmospheric Cold Plasma Treated Wheat Using Cell and Insect Models |
| title_sort | assessing the biological safety of atmospheric cold plasma treated wheat using cell and insect models |
| topic | atmospheric cold plasma food processing wheat grains safety evaluations food model systems cytotoxicity |
| url | https://www.mdpi.com/2304-8158/9/7/898 |
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