First detection and genotyping of Enterocytozoon bieneusi in reindeers (Rangifer tarandus): a zoonotic potential of ITS genotypes
Abstract Background Enterocytozoon bieneusi is the most common pathogen of 14 microsporidian species infecting humans worldwide. In China, E. bieneusi has been reported in some common livestock and environmental specimens. However, no information is available on occurrence of E. bieneusi in reindeer...
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| Format: | Article |
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BMC
2015-10-01
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| Series: | Parasites & Vectors |
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| Online Access: | https://doi.org/10.1186/s13071-015-1155-0 |
| _version_ | 1797811810790801408 |
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| author | Weishi Liu Chunyu Nie Longxian Zhang Rongjun Wang Aiqin Liu Wei Zhao Heping Li |
| author_facet | Weishi Liu Chunyu Nie Longxian Zhang Rongjun Wang Aiqin Liu Wei Zhao Heping Li |
| author_sort | Weishi Liu |
| collection | DOAJ |
| description | Abstract Background Enterocytozoon bieneusi is the most common pathogen of 14 microsporidian species infecting humans worldwide. In China, E. bieneusi has been reported in some common livestock and environmental specimens. However, no information is available on occurrence of E. bieneusi in reindeers. The objective of the present study was to detect and genotype E. bieneusi in reindeers in China, and assess the zoonotic potential. Findings 125 fecal specimens were collected from wild reindeers in the northeast forest region of Great Hinggan Mountains of China. By PCR and sequencing of the internal transcribed spacer (ITS) region of the ribosomal RNA (rRNA) gene of E. bieneusi, an average infection rate of 16.8 % (21/125) was observed in reindeers. E. bieneusi was detected in two age groups: 7.7 % (3/39) in the youths (aged 1 to 2 years) and 22.2 % (18/81) in the adults (aged 3 to 8 years). Five genotypes were identified: one known genotype Peru6 (n = 6) and four novel genotypes named as CHN-RD1 (n = 12), and CHN-RD 2 to CHN-RD4 (one each). In phylogenetic analysis, all the novel genotypes together with known genotype Peru 6 were clustered into group 1. Conclusions This is the first report of E. bieneusi infection in reindeers, expanding the host range of E. bieneusi. The fact of genotype Peru 6 previously reported in humans and the result of all the novel genotypes falling into zoonotic group 1 suggest the possibility of E. bieneusi transmitted from reindeers to humans. |
| first_indexed | 2024-03-13T07:28:15Z |
| format | Article |
| id | doaj.art-45eb06688067487b97a5e926fb28f221 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-03-13T07:28:15Z |
| publishDate | 2015-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-45eb06688067487b97a5e926fb28f2212023-06-04T11:13:00ZengBMCParasites & Vectors1756-33052015-10-01811510.1186/s13071-015-1155-0First detection and genotyping of Enterocytozoon bieneusi in reindeers (Rangifer tarandus): a zoonotic potential of ITS genotypesWeishi Liu0Chunyu Nie1Longxian Zhang2Rongjun Wang3Aiqin Liu4Wei Zhao5Heping Li6College of Wildlife Resources, Northeast Forestry UniversityCollege of Wildlife Resources, Northeast Forestry UniversityCollege of Animal Science and Veterinary Medicine, Henan Agricultural UniversityCollege of Animal Science and Veterinary Medicine, Henan Agricultural UniversityDepartment of Parasitology, Harbin Medical UniversityDepartment of Parasitology, Harbin Medical UniversityCollege of Wildlife Resources, Northeast Forestry UniversityAbstract Background Enterocytozoon bieneusi is the most common pathogen of 14 microsporidian species infecting humans worldwide. In China, E. bieneusi has been reported in some common livestock and environmental specimens. However, no information is available on occurrence of E. bieneusi in reindeers. The objective of the present study was to detect and genotype E. bieneusi in reindeers in China, and assess the zoonotic potential. Findings 125 fecal specimens were collected from wild reindeers in the northeast forest region of Great Hinggan Mountains of China. By PCR and sequencing of the internal transcribed spacer (ITS) region of the ribosomal RNA (rRNA) gene of E. bieneusi, an average infection rate of 16.8 % (21/125) was observed in reindeers. E. bieneusi was detected in two age groups: 7.7 % (3/39) in the youths (aged 1 to 2 years) and 22.2 % (18/81) in the adults (aged 3 to 8 years). Five genotypes were identified: one known genotype Peru6 (n = 6) and four novel genotypes named as CHN-RD1 (n = 12), and CHN-RD 2 to CHN-RD4 (one each). In phylogenetic analysis, all the novel genotypes together with known genotype Peru 6 were clustered into group 1. Conclusions This is the first report of E. bieneusi infection in reindeers, expanding the host range of E. bieneusi. The fact of genotype Peru 6 previously reported in humans and the result of all the novel genotypes falling into zoonotic group 1 suggest the possibility of E. bieneusi transmitted from reindeers to humans.https://doi.org/10.1186/s13071-015-1155-0Enterocytozoon bieneusiITS geneGenotyingReindeersZoonotic |
| spellingShingle | Weishi Liu Chunyu Nie Longxian Zhang Rongjun Wang Aiqin Liu Wei Zhao Heping Li First detection and genotyping of Enterocytozoon bieneusi in reindeers (Rangifer tarandus): a zoonotic potential of ITS genotypes Parasites & Vectors Enterocytozoon bieneusi ITS gene Genotying Reindeers Zoonotic |
| title | First detection and genotyping of Enterocytozoon bieneusi in reindeers (Rangifer tarandus): a zoonotic potential of ITS genotypes |
| title_full | First detection and genotyping of Enterocytozoon bieneusi in reindeers (Rangifer tarandus): a zoonotic potential of ITS genotypes |
| title_fullStr | First detection and genotyping of Enterocytozoon bieneusi in reindeers (Rangifer tarandus): a zoonotic potential of ITS genotypes |
| title_full_unstemmed | First detection and genotyping of Enterocytozoon bieneusi in reindeers (Rangifer tarandus): a zoonotic potential of ITS genotypes |
| title_short | First detection and genotyping of Enterocytozoon bieneusi in reindeers (Rangifer tarandus): a zoonotic potential of ITS genotypes |
| title_sort | first detection and genotyping of enterocytozoon bieneusi in reindeers rangifer tarandus a zoonotic potential of its genotypes |
| topic | Enterocytozoon bieneusi ITS gene Genotying Reindeers Zoonotic |
| url | https://doi.org/10.1186/s13071-015-1155-0 |
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