Genome-Wide Identification and Expression Profile of <i>OSCA</i> Gene Family Members in <i>Triticum aestivum</i> L.
Hyperosmolality and various other stimuli can trigger an increase in cytoplasmic-free calcium concentration ([Ca<sup>2+</sup>]<sub>cyt</sub>). Members of the <i>Arabidopsis thaliana</i> (L.) <i>reduced hyperosmolality-gated calcium-permeable channels</i&g...
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MDPI AG
2021-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/1/469 |
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| author | Kai Tong Xinyang Wu Long He Shiyou Qiu Shuang Liu Linna Cai Shaofei Rao Jianping Chen |
| author_facet | Kai Tong Xinyang Wu Long He Shiyou Qiu Shuang Liu Linna Cai Shaofei Rao Jianping Chen |
| author_sort | Kai Tong |
| collection | DOAJ |
| description | Hyperosmolality and various other stimuli can trigger an increase in cytoplasmic-free calcium concentration ([Ca<sup>2+</sup>]<sub>cyt</sub>). Members of the <i>Arabidopsis thaliana</i> (L.) <i>reduced hyperosmolality-gated calcium-permeable channels</i> (<i>OSCA</i>) gene family are reported to be involved in sensing extracellular changes to trigger hyperosmolality-induced [Ca<sup>2+</sup>]<sub>cyt</sub> increases and controlling stomatal closure during immune signaling. Wheat (<i>Triticum aestivum</i> L.) is a very important food crop, but there are few studies of its <i>OSCA</i> gene family members. In this study, 42 <i>OSCA</i> members were identified in the wheat genome, and phylogenetic analysis can divide them into four clades. The members of each clade have similar gene structures, conserved motifs, and domains. <i>TaOSCA</i> genes were predicted to be regulated by cis-acting elements such as STRE, MBS, DRE1, ABRE, etc. Quantitative PCR results showed that they have different expression patterns in different tissues. The expression profiles of 15 selected <i>TaOSCAs</i> were examined after PEG (polyethylene glycol), NaCl, and ABA (abscisic acid) treatment. All 15 <i>TaOSCA</i> members responded to PEG treatment, while <i>TaOSCA12</i>/-<i>39</i> responded simultaneously to PEG and ABA. This study informs research into the biological function and evolution of <i>TaOSCA</i> and lays the foundation for the breeding and genetic improvement of wheat. |
| first_indexed | 2024-03-10T03:37:28Z |
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| language | English |
| last_indexed | 2024-03-10T03:37:28Z |
| publishDate | 2021-12-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-45ec3fa934434e29b8de651ff34465fc2023-11-23T11:40:29ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-12-0123146910.3390/ijms23010469Genome-Wide Identification and Expression Profile of <i>OSCA</i> Gene Family Members in <i>Triticum aestivum</i> L.Kai Tong0Xinyang Wu1Long He2Shiyou Qiu3Shuang Liu4Linna Cai5Shaofei Rao6Jianping Chen7State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, ChinaCollege of Life Science, China Jiliang University, Hangzhou 310058, ChinaMinistry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, College of Agriculture & Biotechnology, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, ChinaHyperosmolality and various other stimuli can trigger an increase in cytoplasmic-free calcium concentration ([Ca<sup>2+</sup>]<sub>cyt</sub>). Members of the <i>Arabidopsis thaliana</i> (L.) <i>reduced hyperosmolality-gated calcium-permeable channels</i> (<i>OSCA</i>) gene family are reported to be involved in sensing extracellular changes to trigger hyperosmolality-induced [Ca<sup>2+</sup>]<sub>cyt</sub> increases and controlling stomatal closure during immune signaling. Wheat (<i>Triticum aestivum</i> L.) is a very important food crop, but there are few studies of its <i>OSCA</i> gene family members. In this study, 42 <i>OSCA</i> members were identified in the wheat genome, and phylogenetic analysis can divide them into four clades. The members of each clade have similar gene structures, conserved motifs, and domains. <i>TaOSCA</i> genes were predicted to be regulated by cis-acting elements such as STRE, MBS, DRE1, ABRE, etc. Quantitative PCR results showed that they have different expression patterns in different tissues. The expression profiles of 15 selected <i>TaOSCAs</i> were examined after PEG (polyethylene glycol), NaCl, and ABA (abscisic acid) treatment. All 15 <i>TaOSCA</i> members responded to PEG treatment, while <i>TaOSCA12</i>/-<i>39</i> responded simultaneously to PEG and ABA. This study informs research into the biological function and evolution of <i>TaOSCA</i> and lays the foundation for the breeding and genetic improvement of wheat.https://www.mdpi.com/1422-0067/23/1/469abiotic stress<i>reduced hyperosmolality-gated calcium-permeable channels</i>wheatwhole genome expression analysis |
| spellingShingle | Kai Tong Xinyang Wu Long He Shiyou Qiu Shuang Liu Linna Cai Shaofei Rao Jianping Chen Genome-Wide Identification and Expression Profile of <i>OSCA</i> Gene Family Members in <i>Triticum aestivum</i> L. International Journal of Molecular Sciences abiotic stress <i>reduced hyperosmolality-gated calcium-permeable channels</i> wheat whole genome expression analysis |
| title | Genome-Wide Identification and Expression Profile of <i>OSCA</i> Gene Family Members in <i>Triticum aestivum</i> L. |
| title_full | Genome-Wide Identification and Expression Profile of <i>OSCA</i> Gene Family Members in <i>Triticum aestivum</i> L. |
| title_fullStr | Genome-Wide Identification and Expression Profile of <i>OSCA</i> Gene Family Members in <i>Triticum aestivum</i> L. |
| title_full_unstemmed | Genome-Wide Identification and Expression Profile of <i>OSCA</i> Gene Family Members in <i>Triticum aestivum</i> L. |
| title_short | Genome-Wide Identification and Expression Profile of <i>OSCA</i> Gene Family Members in <i>Triticum aestivum</i> L. |
| title_sort | genome wide identification and expression profile of i osca i gene family members in i triticum aestivum i l |
| topic | abiotic stress <i>reduced hyperosmolality-gated calcium-permeable channels</i> wheat whole genome expression analysis |
| url | https://www.mdpi.com/1422-0067/23/1/469 |
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