Development of hydroxyapatite-coated nonwovens for efficient isolation of somatic stem cells from adipose tissues
Adipose-derived stem cells (ASCs) are an attractive cell source for cell therapy. Despite the increasing number of clinical applications, the methodology for ASC isolation is not optimized for every individual. In this study, we developed an effective material to stabilize explant cultures from smal...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Regenerative Therapy |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352320422000505 |
| _version_ | 1811178790020186112 |
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| author | Ryota Chijimatsu Taiga Takeda Shinsaku Tsuji Kohei Sasaki Koichi Kato Rie Kojima Noriko Michihata Toshiya Tsubaki Aya Matui Miharu Watanabe Sakae Tanaka Taku Saito |
| author_facet | Ryota Chijimatsu Taiga Takeda Shinsaku Tsuji Kohei Sasaki Koichi Kato Rie Kojima Noriko Michihata Toshiya Tsubaki Aya Matui Miharu Watanabe Sakae Tanaka Taku Saito |
| author_sort | Ryota Chijimatsu |
| collection | DOAJ |
| description | Adipose-derived stem cells (ASCs) are an attractive cell source for cell therapy. Despite the increasing number of clinical applications, the methodology for ASC isolation is not optimized for every individual. In this study, we developed an effective material to stabilize explant cultures from small-fragment adipose tissues. Methods: Polypropylene/polyethylene nonwoven sheets were coated with hydroxyapatite (HA) particles. Adipose fragments were then placed on these sheets, and their ability to trap tissue was monitored during explant culture. The yield and properties of the cells were compared to those of cells isolated by conventional collagenase digestion. Results: Hydroxyapatite-coated nonwovens immediately trapped adipose fragments when placed on the sheets. The adhesion was stable even in culture media, leading to cell migration and proliferation from the tissue along with the nonwoven fibers. A higher fiber density further enhanced cell growth. Although cells on nonwoven explants could not be fully collected with cell dissociation enzymes, the cell yield was significantly higher than that of conventional monolayer culture without impacting stem cell properties. Conclusions: Hydroxyapatite-coated nonwovens are useful for the effective primary explant culture of connective tissues without enzymatic cell dissociation. |
| first_indexed | 2024-04-11T06:24:58Z |
| format | Article |
| id | doaj.art-45efff1590584f13b4569158d556ec60 |
| institution | Directory Open Access Journal |
| issn | 2352-3204 |
| language | English |
| last_indexed | 2024-04-11T06:24:58Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Regenerative Therapy |
| spelling | doaj.art-45efff1590584f13b4569158d556ec602022-12-22T04:40:24ZengElsevierRegenerative Therapy2352-32042022-12-01215261Development of hydroxyapatite-coated nonwovens for efficient isolation of somatic stem cells from adipose tissuesRyota Chijimatsu0Taiga Takeda1Shinsaku Tsuji2Kohei Sasaki3Koichi Kato4Rie Kojima5Noriko Michihata6Toshiya Tsubaki7Aya Matui8Miharu Watanabe9Sakae Tanaka10Taku Saito11The University of Tokyo, Bone and Cartilage Regenerative Medicine, Graduate School of Medicine, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan; The University of Tokyo, Sensory and Motor System Medicine, Graduate School of Medicine, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan; Okayama University Hospital, Center for Comprehensive Genomic Medicine, 2-5-1, Shikada-chou, Kita-ku, Okayama, 700-8558, Japan; Corresponding author. Okayama University Hospital, Center for Comprehensive Genomic Medicine, 2-5-1, Shikada-chou, Kita-ku, Okayama, 700-8558, JapanThe University of Tokyo, Bone and Cartilage Regenerative Medicine, Graduate School of Medicine, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan; CPC Corporation, 3-18-16 Minami-Aoyama, Minato-ku, Tokyo, 107-0062, JapanCPC Corporation, 3-18-16 Minami-Aoyama, Minato-ku, Tokyo, 107-0062, Japan; Avenue Cell Clinic, 3-18-16 Minami-Aoyama, Minato-ku, Tokyo, 107-0062, JapanJapan Vilene Company, Ltd., Central Research Laboratory, 7 Kita-tone, Koga-shi, Ibaraki, 306-0213, JapanJapan Vilene Company, Ltd., Central Research Laboratory, 7 Kita-tone, Koga-shi, Ibaraki, 306-0213, JapanJapan Vilene Company, Ltd., Central Research Laboratory, 7 Kita-tone, Koga-shi, Ibaraki, 306-0213, JapanJapan Vilene Company, Ltd., Central Research Laboratory, 7 Kita-tone, Koga-shi, Ibaraki, 306-0213, JapanThe University of Tokyo, Sensory and Motor System Medicine, Graduate School of Medicine, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, JapanCPC Corporation, 3-18-16 Minami-Aoyama, Minato-ku, Tokyo, 107-0062, JapanCPC Corporation, 3-18-16 Minami-Aoyama, Minato-ku, Tokyo, 107-0062, JapanThe University of Tokyo, Sensory and Motor System Medicine, Graduate School of Medicine, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, JapanThe University of Tokyo, Sensory and Motor System Medicine, Graduate School of Medicine, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, JapanAdipose-derived stem cells (ASCs) are an attractive cell source for cell therapy. Despite the increasing number of clinical applications, the methodology for ASC isolation is not optimized for every individual. In this study, we developed an effective material to stabilize explant cultures from small-fragment adipose tissues. Methods: Polypropylene/polyethylene nonwoven sheets were coated with hydroxyapatite (HA) particles. Adipose fragments were then placed on these sheets, and their ability to trap tissue was monitored during explant culture. The yield and properties of the cells were compared to those of cells isolated by conventional collagenase digestion. Results: Hydroxyapatite-coated nonwovens immediately trapped adipose fragments when placed on the sheets. The adhesion was stable even in culture media, leading to cell migration and proliferation from the tissue along with the nonwoven fibers. A higher fiber density further enhanced cell growth. Although cells on nonwoven explants could not be fully collected with cell dissociation enzymes, the cell yield was significantly higher than that of conventional monolayer culture without impacting stem cell properties. Conclusions: Hydroxyapatite-coated nonwovens are useful for the effective primary explant culture of connective tissues without enzymatic cell dissociation.http://www.sciencedirect.com/science/article/pii/S2352320422000505Adipose stem cellsExplant cultureNonwovensHydroxyapatite |
| spellingShingle | Ryota Chijimatsu Taiga Takeda Shinsaku Tsuji Kohei Sasaki Koichi Kato Rie Kojima Noriko Michihata Toshiya Tsubaki Aya Matui Miharu Watanabe Sakae Tanaka Taku Saito Development of hydroxyapatite-coated nonwovens for efficient isolation of somatic stem cells from adipose tissues Regenerative Therapy Adipose stem cells Explant culture Nonwovens Hydroxyapatite |
| title | Development of hydroxyapatite-coated nonwovens for efficient isolation of somatic stem cells from adipose tissues |
| title_full | Development of hydroxyapatite-coated nonwovens for efficient isolation of somatic stem cells from adipose tissues |
| title_fullStr | Development of hydroxyapatite-coated nonwovens for efficient isolation of somatic stem cells from adipose tissues |
| title_full_unstemmed | Development of hydroxyapatite-coated nonwovens for efficient isolation of somatic stem cells from adipose tissues |
| title_short | Development of hydroxyapatite-coated nonwovens for efficient isolation of somatic stem cells from adipose tissues |
| title_sort | development of hydroxyapatite coated nonwovens for efficient isolation of somatic stem cells from adipose tissues |
| topic | Adipose stem cells Explant culture Nonwovens Hydroxyapatite |
| url | http://www.sciencedirect.com/science/article/pii/S2352320422000505 |
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