Expression and localization of PIWI proteins in testis and ovary of domestic sheep
The piRNA-PIWI protein complex plays crucial roles in safeguarding the genome against inordinate transposon mobilization and regulation of embryonic development. A previous study indicated the presence of piRNA in sheep reproductive organs. However, the tissue distribution and cellular localization...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2020-03-01
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| Series: | Czech Journal of Animal Science |
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| Online Access: | https://cjas.agriculturejournals.cz/artkey/cjs-202003-0002_expression-and-localization-of-piwi-proteins-in-testis-and-ovary-of-domestic-sheep.php |
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| author | Chunyan Li Qiuyue Liu Xiangyu Wang Wenping Hu Deping Han Joram Mwashigadi Mwacharo Caihong Wei Mingxing Chu Ran Di |
| author_facet | Chunyan Li Qiuyue Liu Xiangyu Wang Wenping Hu Deping Han Joram Mwashigadi Mwacharo Caihong Wei Mingxing Chu Ran Di |
| author_sort | Chunyan Li |
| collection | DOAJ |
| description | The piRNA-PIWI protein complex plays crucial roles in safeguarding the genome against inordinate transposon mobilization and regulation of embryonic development. A previous study indicated the presence of piRNA in sheep reproductive organs. However, the tissue distribution and cellular localization of PIWI proteins in sheep remains unclear. Therefore the present study aimed to explore the expression profiles of mRNAs of mammalian PIWI proteins (PIWIL1, PIWIL2, PIWIL4 and AGO3) in 9 tissues derived from adult male and female sheep. Results showed the expression of PIWIL1, PIWIL2, and PIWIL4 was significantly higher in the testis and ovary than in the other tissues. Immunohistochemistry analysis of testes indicated that each of the 4 proteins had specific cellular localizations, and some of the localizations were different from those of other species. All the proteins were mainly localized in the primary spermatocytes, suggesting that they are crucial for silencing of transposon to guarantee the integrity of the gamete genome during early stage of spermatogenesis. In the ovaries, the PIWI proteins were mainly localized in oocytes from antral follicles and leukocytes in ovarian blood. Our results provide insights to better understand the functions of PIWI proteins during spermatogenesis, oogenesis and immune defence in sheep. |
| first_indexed | 2024-04-10T08:24:56Z |
| format | Article |
| id | doaj.art-45f1294b63c141d3b8eefef6d458dfc1 |
| institution | Directory Open Access Journal |
| issn | 1212-1819 1805-9309 |
| language | English |
| last_indexed | 2024-04-10T08:24:56Z |
| publishDate | 2020-03-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Animal Science |
| spelling | doaj.art-45f1294b63c141d3b8eefef6d458dfc12023-02-23T03:33:44ZengCzech Academy of Agricultural SciencesCzech Journal of Animal Science1212-18191805-93092020-03-01653869610.17221/7/2020-CJAScjs-202003-0002Expression and localization of PIWI proteins in testis and ovary of domestic sheepChunyan Li0Qiuyue Liu1Xiangyu Wang2Wenping Hu3Deping Han4Joram Mwashigadi Mwacharo5Caihong Wei6Mingxing Chu7Ran Di8Key Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P. R. ChinaKey Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P. R. ChinaKey Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P. R. ChinaKey Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P. R. ChinaCollege of Veterinary Medicine, China Agricultural University, Beijing, P. R. ChinaSmall Ruminant Genomics, International Centre for Agricultural Research in the Dry Areas (ICARDA), Addis Ababa, EthiopiaKey Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P. R. ChinaKey Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P. R. ChinaKey Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P. R. ChinaThe piRNA-PIWI protein complex plays crucial roles in safeguarding the genome against inordinate transposon mobilization and regulation of embryonic development. A previous study indicated the presence of piRNA in sheep reproductive organs. However, the tissue distribution and cellular localization of PIWI proteins in sheep remains unclear. Therefore the present study aimed to explore the expression profiles of mRNAs of mammalian PIWI proteins (PIWIL1, PIWIL2, PIWIL4 and AGO3) in 9 tissues derived from adult male and female sheep. Results showed the expression of PIWIL1, PIWIL2, and PIWIL4 was significantly higher in the testis and ovary than in the other tissues. Immunohistochemistry analysis of testes indicated that each of the 4 proteins had specific cellular localizations, and some of the localizations were different from those of other species. All the proteins were mainly localized in the primary spermatocytes, suggesting that they are crucial for silencing of transposon to guarantee the integrity of the gamete genome during early stage of spermatogenesis. In the ovaries, the PIWI proteins were mainly localized in oocytes from antral follicles and leukocytes in ovarian blood. Our results provide insights to better understand the functions of PIWI proteins during spermatogenesis, oogenesis and immune defence in sheep.https://cjas.agriculturejournals.cz/artkey/cjs-202003-0002_expression-and-localization-of-piwi-proteins-in-testis-and-ovary-of-domestic-sheep.phppiwi proteinsreproductionlocalizationtestisovary |
| spellingShingle | Chunyan Li Qiuyue Liu Xiangyu Wang Wenping Hu Deping Han Joram Mwashigadi Mwacharo Caihong Wei Mingxing Chu Ran Di Expression and localization of PIWI proteins in testis and ovary of domestic sheep Czech Journal of Animal Science piwi proteins reproduction localization testis ovary |
| title | Expression and localization of PIWI proteins in testis and ovary of domestic sheep |
| title_full | Expression and localization of PIWI proteins in testis and ovary of domestic sheep |
| title_fullStr | Expression and localization of PIWI proteins in testis and ovary of domestic sheep |
| title_full_unstemmed | Expression and localization of PIWI proteins in testis and ovary of domestic sheep |
| title_short | Expression and localization of PIWI proteins in testis and ovary of domestic sheep |
| title_sort | expression and localization of piwi proteins in testis and ovary of domestic sheep |
| topic | piwi proteins reproduction localization testis ovary |
| url | https://cjas.agriculturejournals.cz/artkey/cjs-202003-0002_expression-and-localization-of-piwi-proteins-in-testis-and-ovary-of-domestic-sheep.php |
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