Diagnostic efficacy of rapid assays used for the detection of hepatitis B virus surface antigen
<p><strong>Objectives:</strong> Two rapid tests, <em>CORTEZ’S HBsAg one step</em> and <em>CTK Biotech’s Onsite HBsAg, </em>for the detection of HBsAg in the serum were compared using the SURASE B-96 (TMB) enzyme immunoassay (EIA) as the comparator.</p>...
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| Format: | Article |
| Language: | English |
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Sri Lankan Society for Microbiology
2013-07-01
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| Series: | Sri Lankan Journal of Infectious Diseases |
| Subjects: | |
| Online Access: | https://sljid.sljol.info/articles/5172 |
| _version_ | 1828332873610952704 |
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| author | EWS Chameera F Noordeen H Pandithasundara AMSB Abeykoon |
| author_facet | EWS Chameera F Noordeen H Pandithasundara AMSB Abeykoon |
| author_sort | EWS Chameera |
| collection | DOAJ |
| description | <p><strong>Objectives:</strong> Two rapid tests, <em>CORTEZ’S HBsAg one step</em> and <em>CTK Biotech’s Onsite HBsAg, </em>for the detection of HBsAg in the serum were compared using the SURASE B-96 (TMB) enzyme immunoassay (EIA) as the comparator.</p><p><strong>Methods:</strong> Fifty blood samples (n=50) were tested using the two rapid tests and the EIA.</p><p><strong></strong><strong>Results:</strong> Based on the EIA, 5 sera were positive for HBsAg and 45 sera were negative for HBsAg. However, 4 sera were positive while 46 sera were negative for HBsAg when tested by Biotech’s Onsite HBsAg. Three (n=3) sera were positive while 47 sera were negative for HBsAg when tested by CORTEZ’S HBsAg one step detection kit. The specificity and the positive predictive value (PPV) were 100% for both rapid tests. However, the sensitivity and negative predictive value (NPV) for Biotech’s Onsite HBsAg assay and CORTEZ’S HBsAg one step assay were 80% and 97.82% and 60% and 95.74%, respectively.</p><p><strong></strong><strong>Conclusions:</strong> Both rapid tests showed less sensitivity and NPV than the EIA. Sensitivity and NPV were higher with <em>Onsite HBsAg</em> than the <em>CORTEZ’S HBsAg</em> making the former slightly better than the latter for detecting serum HBsAg. According to our findings, the accuracy of rapid tests for HBsAg detection varied compared to EIA, suggesting the importance of validating rapid tests routinely when used in diagnostic laboratories.</p><p class="Default">DOI: <a href="http://dx.doi.org/10.4038/sljid.v3i2.5172">http://dx.doi.org/10.4038/sljid.v3i2.5172</a></p> <p class="Default"><em>Sri Lankan Journal of Infectious Diseases 2013; Vol.3(2):21-27</em></p> |
| first_indexed | 2024-04-13T21:13:11Z |
| format | Article |
| id | doaj.art-45f34b73d243477fac3a71c190f353e3 |
| institution | Directory Open Access Journal |
| issn | 2012-8169 2448-9654 |
| language | English |
| last_indexed | 2024-04-13T21:13:11Z |
| publishDate | 2013-07-01 |
| publisher | Sri Lankan Society for Microbiology |
| record_format | Article |
| series | Sri Lankan Journal of Infectious Diseases |
| spelling | doaj.art-45f34b73d243477fac3a71c190f353e32022-12-22T02:29:47ZengSri Lankan Society for MicrobiologySri Lankan Journal of Infectious Diseases2012-81692448-96542013-07-0132212710.4038/sljid.v3i2.51724625Diagnostic efficacy of rapid assays used for the detection of hepatitis B virus surface antigenEWS Chameera0F Noordeen1H Pandithasundara2AMSB Abeykoon3Faculty of Medicine, University of Peradeniya.Faculty of Medicine, University of PeradeniyaFaculty of Medicine, University of PeradeniyaFaculty of Medicine, University of Peradeniya<p><strong>Objectives:</strong> Two rapid tests, <em>CORTEZ’S HBsAg one step</em> and <em>CTK Biotech’s Onsite HBsAg, </em>for the detection of HBsAg in the serum were compared using the SURASE B-96 (TMB) enzyme immunoassay (EIA) as the comparator.</p><p><strong>Methods:</strong> Fifty blood samples (n=50) were tested using the two rapid tests and the EIA.</p><p><strong></strong><strong>Results:</strong> Based on the EIA, 5 sera were positive for HBsAg and 45 sera were negative for HBsAg. However, 4 sera were positive while 46 sera were negative for HBsAg when tested by Biotech’s Onsite HBsAg. Three (n=3) sera were positive while 47 sera were negative for HBsAg when tested by CORTEZ’S HBsAg one step detection kit. The specificity and the positive predictive value (PPV) were 100% for both rapid tests. However, the sensitivity and negative predictive value (NPV) for Biotech’s Onsite HBsAg assay and CORTEZ’S HBsAg one step assay were 80% and 97.82% and 60% and 95.74%, respectively.</p><p><strong></strong><strong>Conclusions:</strong> Both rapid tests showed less sensitivity and NPV than the EIA. Sensitivity and NPV were higher with <em>Onsite HBsAg</em> than the <em>CORTEZ’S HBsAg</em> making the former slightly better than the latter for detecting serum HBsAg. According to our findings, the accuracy of rapid tests for HBsAg detection varied compared to EIA, suggesting the importance of validating rapid tests routinely when used in diagnostic laboratories.</p><p class="Default">DOI: <a href="http://dx.doi.org/10.4038/sljid.v3i2.5172">http://dx.doi.org/10.4038/sljid.v3i2.5172</a></p> <p class="Default"><em>Sri Lankan Journal of Infectious Diseases 2013; Vol.3(2):21-27</em></p>https://sljid.sljol.info/articles/5172rapid immunochromatography assayshepatitis b virusserum hbsagenzyme immunoassays |
| spellingShingle | EWS Chameera F Noordeen H Pandithasundara AMSB Abeykoon Diagnostic efficacy of rapid assays used for the detection of hepatitis B virus surface antigen Sri Lankan Journal of Infectious Diseases rapid immunochromatography assays hepatitis b virus serum hbsag enzyme immunoassays |
| title | Diagnostic efficacy of rapid assays used for the detection of hepatitis B virus surface antigen |
| title_full | Diagnostic efficacy of rapid assays used for the detection of hepatitis B virus surface antigen |
| title_fullStr | Diagnostic efficacy of rapid assays used for the detection of hepatitis B virus surface antigen |
| title_full_unstemmed | Diagnostic efficacy of rapid assays used for the detection of hepatitis B virus surface antigen |
| title_short | Diagnostic efficacy of rapid assays used for the detection of hepatitis B virus surface antigen |
| title_sort | diagnostic efficacy of rapid assays used for the detection of hepatitis b virus surface antigen |
| topic | rapid immunochromatography assays hepatitis b virus serum hbsag enzyme immunoassays |
| url | https://sljid.sljol.info/articles/5172 |
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