Fecal Metaproteomic Analysis Reveals Unique Changes of the Gut Microbiome Functions After Consumption of Sourdough Carasau Bread
Sourdough-leavened bread (SB) is acknowledged for its great variety of valuable effects on consumer's metabolism and health, including a low glycemic index and a reduced content of the possible carcinogen acrylamide. Here, we aimed to investigate how these effects influence the gut microbiota c...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2019-07-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.01733/full |
| _version_ | 1818897786595180544 |
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| author | Marcello Abbondio Antonio Palomba Alessandro Tanca Cristina Fraumene Daniela Pagnozzi Monica Serra Fabio Marongiu Ezio Laconi Sergio Uzzau Sergio Uzzau |
| author_facet | Marcello Abbondio Antonio Palomba Alessandro Tanca Cristina Fraumene Daniela Pagnozzi Monica Serra Fabio Marongiu Ezio Laconi Sergio Uzzau Sergio Uzzau |
| author_sort | Marcello Abbondio |
| collection | DOAJ |
| description | Sourdough-leavened bread (SB) is acknowledged for its great variety of valuable effects on consumer's metabolism and health, including a low glycemic index and a reduced content of the possible carcinogen acrylamide. Here, we aimed to investigate how these effects influence the gut microbiota composition and functions. Therefore, we subjected rats to a diet supplemented with SB, baker's yeast leavened bread (BB), or unsupplemented diet (chow), and, after 4 weeks of treatment, their gut microbiota was analyzed using a metaproteogenomic approach. As a result, diet supplementation with SB led to a reduction of specific members of the intestinal microbiota previously associated to low protein diets, namely Alistipes and Mucispirillum, or known as intestinal pathobionts, i.e., Mycoplasma. Concerning functions, asparaginases expressed by Bacteroides were observed as more abundant in SB-fed rats, leading to hypothesize that in their colonic microbiota the enzyme substrate, asparagine, was available in higher amounts than in BB- and chow-fed rats. Another group of protein families, expressed by Clostridium, was detected as more abundant in animal fed SB-supplemented diet. Of these, manganese catalase, small acid-soluble proteins (SASP), Ser/Thr kinase PrkA, and V-ATPase proteolipid subunit have been all reported to take part in Clostridium sporulation, strongly suggesting that the diet supplementation with SB might promote environmental conditions inducing metabolic dormancy of Clostridium spp. within the gut microbiota. In conclusion, our data describe the effects of SB consumption on the intestinal microbiota taxonomy and functions in rats. Moreover, our results suggest that a metaproteogenomic approach can provide evidence of the interplay between metabolites deriving from bread digestion and microbial metabolism. |
| first_indexed | 2024-12-19T19:21:43Z |
| format | Article |
| id | doaj.art-45f6f24eb4394bdca9dc562b100aca42 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-19T19:21:43Z |
| publishDate | 2019-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-45f6f24eb4394bdca9dc562b100aca422022-12-21T20:08:58ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-07-011010.3389/fmicb.2019.01733465686Fecal Metaproteomic Analysis Reveals Unique Changes of the Gut Microbiome Functions After Consumption of Sourdough Carasau BreadMarcello Abbondio0Antonio Palomba1Alessandro Tanca2Cristina Fraumene3Daniela Pagnozzi4Monica Serra5Fabio Marongiu6Ezio Laconi7Sergio Uzzau8Sergio Uzzau9Department of Biomedical Sciences, University of Sassari, Sassari, ItalyPorto Conte Ricerche, Science and Technology Park of Sardinia, Alghero, ItalyPorto Conte Ricerche, Science and Technology Park of Sardinia, Alghero, ItalyPorto Conte Ricerche, Science and Technology Park of Sardinia, Alghero, ItalyPorto Conte Ricerche, Science and Technology Park of Sardinia, Alghero, ItalyDepartment of Biomedical Sciences, University of Cagliari, Cagliari, ItalyDepartment of Biomedical Sciences, University of Cagliari, Cagliari, ItalyDepartment of Biomedical Sciences, University of Cagliari, Cagliari, ItalyDepartment of Biomedical Sciences, University of Sassari, Sassari, ItalyPorto Conte Ricerche, Science and Technology Park of Sardinia, Alghero, ItalySourdough-leavened bread (SB) is acknowledged for its great variety of valuable effects on consumer's metabolism and health, including a low glycemic index and a reduced content of the possible carcinogen acrylamide. Here, we aimed to investigate how these effects influence the gut microbiota composition and functions. Therefore, we subjected rats to a diet supplemented with SB, baker's yeast leavened bread (BB), or unsupplemented diet (chow), and, after 4 weeks of treatment, their gut microbiota was analyzed using a metaproteogenomic approach. As a result, diet supplementation with SB led to a reduction of specific members of the intestinal microbiota previously associated to low protein diets, namely Alistipes and Mucispirillum, or known as intestinal pathobionts, i.e., Mycoplasma. Concerning functions, asparaginases expressed by Bacteroides were observed as more abundant in SB-fed rats, leading to hypothesize that in their colonic microbiota the enzyme substrate, asparagine, was available in higher amounts than in BB- and chow-fed rats. Another group of protein families, expressed by Clostridium, was detected as more abundant in animal fed SB-supplemented diet. Of these, manganese catalase, small acid-soluble proteins (SASP), Ser/Thr kinase PrkA, and V-ATPase proteolipid subunit have been all reported to take part in Clostridium sporulation, strongly suggesting that the diet supplementation with SB might promote environmental conditions inducing metabolic dormancy of Clostridium spp. within the gut microbiota. In conclusion, our data describe the effects of SB consumption on the intestinal microbiota taxonomy and functions in rats. Moreover, our results suggest that a metaproteogenomic approach can provide evidence of the interplay between metabolites deriving from bread digestion and microbial metabolism.https://www.frontiersin.org/article/10.3389/fmicb.2019.01733/fullgut microbiotametagenomicsmetaproteomicsfood processessourdoughdiet |
| spellingShingle | Marcello Abbondio Antonio Palomba Alessandro Tanca Cristina Fraumene Daniela Pagnozzi Monica Serra Fabio Marongiu Ezio Laconi Sergio Uzzau Sergio Uzzau Fecal Metaproteomic Analysis Reveals Unique Changes of the Gut Microbiome Functions After Consumption of Sourdough Carasau Bread Frontiers in Microbiology gut microbiota metagenomics metaproteomics food processes sourdough diet |
| title | Fecal Metaproteomic Analysis Reveals Unique Changes of the Gut Microbiome Functions After Consumption of Sourdough Carasau Bread |
| title_full | Fecal Metaproteomic Analysis Reveals Unique Changes of the Gut Microbiome Functions After Consumption of Sourdough Carasau Bread |
| title_fullStr | Fecal Metaproteomic Analysis Reveals Unique Changes of the Gut Microbiome Functions After Consumption of Sourdough Carasau Bread |
| title_full_unstemmed | Fecal Metaproteomic Analysis Reveals Unique Changes of the Gut Microbiome Functions After Consumption of Sourdough Carasau Bread |
| title_short | Fecal Metaproteomic Analysis Reveals Unique Changes of the Gut Microbiome Functions After Consumption of Sourdough Carasau Bread |
| title_sort | fecal metaproteomic analysis reveals unique changes of the gut microbiome functions after consumption of sourdough carasau bread |
| topic | gut microbiota metagenomics metaproteomics food processes sourdough diet |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.01733/full |
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