Population dynamics and antigenic drift of Bordetella pertussis following whole cell vaccine replacement, Barcelona, Spain, 1986–2015
ABSTRACTAmong the factors associated with the resurgence of whooping cough, special emphasis has been given to pathogen adaptation after the introduction of the acellular vaccine (ACV). To assess the impact of the vaccine transition strategy from whole-cell vaccine (WCV) to ACV on population dynamic...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-01-01
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| Series: | Emerging Microbes and Infections |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2019.1694395 |
| _version_ | 1797385755487633408 |
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| author | Alba Mir-Cros Albert Moreno-Mingorance M. Teresa Martín-Gómez Gema Codina Thais Cornejo-Sánchez Mireia Rajadell Diego Van Esso Carlos Rodrigo Magda Campins Mireia Jané Tomàs Pumarola Anna Fàbrega Juan José González-López |
| author_facet | Alba Mir-Cros Albert Moreno-Mingorance M. Teresa Martín-Gómez Gema Codina Thais Cornejo-Sánchez Mireia Rajadell Diego Van Esso Carlos Rodrigo Magda Campins Mireia Jané Tomàs Pumarola Anna Fàbrega Juan José González-López |
| author_sort | Alba Mir-Cros |
| collection | DOAJ |
| description | ABSTRACTAmong the factors associated with the resurgence of whooping cough, special emphasis has been given to pathogen adaptation after the introduction of the acellular vaccine (ACV). To assess the impact of the vaccine transition strategy from whole-cell vaccine (WCV) to ACV on population dynamics of Bordetella pertussis in Barcelona (Spain), we studied 339 isolates collected from 1986 to 2015 by PFGE and multi-locus variable-number tandem repeat analysis (MLVA). Additionally, allelic variants for the pertussis toxin and its promoter, pertactin, type 3 fimbriae and fimbrial serotyping were assessed to determine its antigenic drift. A shift was observed in the B. pertussis population as well as in its antigenic profile concurrently with the introduction of ACV in Barcelona. Four out of the five most prevalent PFGE profiles were replaced by new profiles following the ACV introduction. MLVA type 27 was the dominant genotype, and its frequency increased from 25% to 79.3% after WCV replacement. Antigen typing demonstrated the emergence of prn2, ptxP3, fim3-2 and a shift from the fimbriae 3 to the fimbriae 2 serotypes after the ACV introduction. Our findings support the presence of population and antigenic dynamic changes in B. pertussis likely driven by the introduction of ACV. |
| first_indexed | 2024-03-08T21:58:50Z |
| format | Article |
| id | doaj.art-45f7a6d7cebd49e4bc1ddb84a2b6ebde |
| institution | Directory Open Access Journal |
| issn | 2222-1751 |
| language | English |
| last_indexed | 2024-03-08T21:58:50Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj.art-45f7a6d7cebd49e4bc1ddb84a2b6ebde2023-12-19T16:09:58ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512019-01-01811711172010.1080/22221751.2019.1694395Population dynamics and antigenic drift of Bordetella pertussis following whole cell vaccine replacement, Barcelona, Spain, 1986–2015Alba Mir-Cros0Albert Moreno-Mingorance1M. Teresa Martín-Gómez2Gema Codina3Thais Cornejo-Sánchez4Mireia Rajadell5Diego Van Esso6Carlos Rodrigo7Magda Campins8Mireia Jané9Tomàs Pumarola10Anna Fàbrega11Juan José González-López12Department of Clinical Microbiology, Hospital Universitari Vall d'Hebron, Barcelona, SpainDepartment of Clinical Microbiology, Hospital Universitari Vall d'Hebron, Barcelona, SpainDepartment of Clinical Microbiology, Hospital Universitari Vall d'Hebron, Barcelona, SpainDepartment of Clinical Microbiology, Hospital Universitari Vall d'Hebron, Barcelona, SpainDepartment of Clinical Microbiology, Hospital Universitari Vall d'Hebron, Barcelona, SpainDepartment of Clinical Microbiology, Hospital Universitari Vall d'Hebron, Barcelona, SpainPrimary Care Health Centre Service 'Muntanya', Catalan Institute of Health, Barcelona, SpainDepartment of Paediatrics, Hospital Universitari Vall d'Hebron, Barcelona, SpainDepartment of Preventive Medicine and Epidemiology, Hospital Universitari Vall d'Hebron, Barcelona, SpainPublic Health Agency of Catalonia, Barcelona, SpainDepartment of Clinical Microbiology, Hospital Universitari Vall d'Hebron, Barcelona, SpainDepartment of Clinical Microbiology, Hospital Universitari Vall d'Hebron, Barcelona, SpainDepartment of Clinical Microbiology, Hospital Universitari Vall d'Hebron, Barcelona, SpainABSTRACTAmong the factors associated with the resurgence of whooping cough, special emphasis has been given to pathogen adaptation after the introduction of the acellular vaccine (ACV). To assess the impact of the vaccine transition strategy from whole-cell vaccine (WCV) to ACV on population dynamics of Bordetella pertussis in Barcelona (Spain), we studied 339 isolates collected from 1986 to 2015 by PFGE and multi-locus variable-number tandem repeat analysis (MLVA). Additionally, allelic variants for the pertussis toxin and its promoter, pertactin, type 3 fimbriae and fimbrial serotyping were assessed to determine its antigenic drift. A shift was observed in the B. pertussis population as well as in its antigenic profile concurrently with the introduction of ACV in Barcelona. Four out of the five most prevalent PFGE profiles were replaced by new profiles following the ACV introduction. MLVA type 27 was the dominant genotype, and its frequency increased from 25% to 79.3% after WCV replacement. Antigen typing demonstrated the emergence of prn2, ptxP3, fim3-2 and a shift from the fimbriae 3 to the fimbriae 2 serotypes after the ACV introduction. Our findings support the presence of population and antigenic dynamic changes in B. pertussis likely driven by the introduction of ACV.https://www.tandfonline.com/doi/10.1080/22221751.2019.1694395Whooping coughPFGEMLVAantigenic variantspertussis vaccine |
| spellingShingle | Alba Mir-Cros Albert Moreno-Mingorance M. Teresa Martín-Gómez Gema Codina Thais Cornejo-Sánchez Mireia Rajadell Diego Van Esso Carlos Rodrigo Magda Campins Mireia Jané Tomàs Pumarola Anna Fàbrega Juan José González-López Population dynamics and antigenic drift of Bordetella pertussis following whole cell vaccine replacement, Barcelona, Spain, 1986–2015 Emerging Microbes and Infections Whooping cough PFGE MLVA antigenic variants pertussis vaccine |
| title | Population dynamics and antigenic drift of Bordetella pertussis following whole cell vaccine replacement, Barcelona, Spain, 1986–2015 |
| title_full | Population dynamics and antigenic drift of Bordetella pertussis following whole cell vaccine replacement, Barcelona, Spain, 1986–2015 |
| title_fullStr | Population dynamics and antigenic drift of Bordetella pertussis following whole cell vaccine replacement, Barcelona, Spain, 1986–2015 |
| title_full_unstemmed | Population dynamics and antigenic drift of Bordetella pertussis following whole cell vaccine replacement, Barcelona, Spain, 1986–2015 |
| title_short | Population dynamics and antigenic drift of Bordetella pertussis following whole cell vaccine replacement, Barcelona, Spain, 1986–2015 |
| title_sort | population dynamics and antigenic drift of bordetella pertussis following whole cell vaccine replacement barcelona spain 1986 2015 |
| topic | Whooping cough PFGE MLVA antigenic variants pertussis vaccine |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2019.1694395 |
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