HISTOLOGICAL ASPECTS OF THE MEDULLARY BONE CORRELATED TO DIFFERENT STAGES OF THE EGG FORMATION IN HENS
The experiment was carried out on a batch formed by 10 laying hens, ISA Brown hybrid, at the first laying cycles, 50 weeks old, hold single in cages and fed with granulated forage. The hens were watched for 3 weeks in order to establish the moment of the oviposition and depending on that their slaug...
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| Format: | Article |
| Language: | English |
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Agroprint Timisoara
2023-11-01
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| Series: | Scientific Papers Animal Science and Biotechnologies |
| Subjects: | |
| Online Access: | https://spasb.ro/index.php/public_html/article/view/1789 |
| _version_ | 1797216624772644864 |
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| author | ALINA GHIȘE MARIANA ŞINCAI DIANA ARGHERIE OLGA RADA GABI DUMITRESCU RODICA ZEHAN |
| author_facet | ALINA GHIȘE MARIANA ŞINCAI DIANA ARGHERIE OLGA RADA GABI DUMITRESCU RODICA ZEHAN |
| author_sort | ALINA GHIȘE |
| collection | DOAJ |
| description | The experiment was carried out on a batch formed by 10 laying hens, ISA Brown
hybrid, at the first laying cycles, 50 weeks old, hold single in cages and fed with
granulated forage. The hens were watched for 3 weeks in order to establish the
moment of the oviposition and depending on that their slaughtering was set out so
that the different stages of the egg formation could be observed. The hens were
divided into 4 groups – I, II, III, IV- depending on the time elapsed from the last
oviposition. Femur fragments have been drawn from the central zone of the
diaphises and transformed in hematoxiline-eozine and alcian-blue stained
preparations, the trichromic Mallory method and the Dorfmann-Epstein method for
the emphasising of the alkaline phosphatasis. At the hens of the first group the
presence of the medullary bone is shown and is characterised by an intense
ossification process with compact bone structure, with well formed osseus
trabeculae that occupy the medullar cavity. There is also present a rich content of
acid mucopolyssacharide. The hens from the second group (II) have a well formed
medullary bone, with visible osseus trabeculae. From the histological point of view,
the medullary bone of the hens from the third group (III) is rarefied with large
areolae, with collagen fibres that lack osein, having thus a characteristic aspect of
massive decalcification. At the hens from the fourth group (IV) the medullary bone
shows synthesis processes and bone matrix forming, positive, intensive FAL activity,
that proves the presence of active osteoblasts, i.e. bone remodelling processes. |
| first_indexed | 2024-04-24T11:48:56Z |
| format | Article |
| id | doaj.art-45f7ab4399e04d15824ecb985f9e4bdf |
| institution | Directory Open Access Journal |
| issn | 1841-9364 2344-4576 |
| language | English |
| last_indexed | 2024-04-24T11:48:56Z |
| publishDate | 2023-11-01 |
| publisher | Agroprint Timisoara |
| record_format | Article |
| series | Scientific Papers Animal Science and Biotechnologies |
| spelling | doaj.art-45f7ab4399e04d15824ecb985f9e4bdf2024-04-09T08:38:11ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-11-014114144141789HISTOLOGICAL ASPECTS OF THE MEDULLARY BONE CORRELATED TO DIFFERENT STAGES OF THE EGG FORMATION IN HENSALINA GHIȘE0MARIANA ŞINCAI1DIANA ARGHERIE2OLGA RADA3GABI DUMITRESCU4RODICA ZEHAN5Faculty of Veterinary Medicine, Timişoara, Romania Faculty of Veterinary Medicine, Timişoara, Romania Faculty of Veterinary Medicine, Timişoara, Romania Faculty of Agriculture, Timişoara, Romania Faculty of Animal Sciences and Biotechnologies, Timişoara, Romania Faculty of Agriculture, Timişoara, Romania The experiment was carried out on a batch formed by 10 laying hens, ISA Brown hybrid, at the first laying cycles, 50 weeks old, hold single in cages and fed with granulated forage. The hens were watched for 3 weeks in order to establish the moment of the oviposition and depending on that their slaughtering was set out so that the different stages of the egg formation could be observed. The hens were divided into 4 groups – I, II, III, IV- depending on the time elapsed from the last oviposition. Femur fragments have been drawn from the central zone of the diaphises and transformed in hematoxiline-eozine and alcian-blue stained preparations, the trichromic Mallory method and the Dorfmann-Epstein method for the emphasising of the alkaline phosphatasis. At the hens of the first group the presence of the medullary bone is shown and is characterised by an intense ossification process with compact bone structure, with well formed osseus trabeculae that occupy the medullar cavity. There is also present a rich content of acid mucopolyssacharide. The hens from the second group (II) have a well formed medullary bone, with visible osseus trabeculae. From the histological point of view, the medullary bone of the hens from the third group (III) is rarefied with large areolae, with collagen fibres that lack osein, having thus a characteristic aspect of massive decalcification. At the hens from the fourth group (IV) the medullary bone shows synthesis processes and bone matrix forming, positive, intensive FAL activity, that proves the presence of active osteoblasts, i.e. bone remodelling processes.https://spasb.ro/index.php/public_html/article/view/1789laying fowlmedullary boneosteoclastosteoblast |
| spellingShingle | ALINA GHIȘE MARIANA ŞINCAI DIANA ARGHERIE OLGA RADA GABI DUMITRESCU RODICA ZEHAN HISTOLOGICAL ASPECTS OF THE MEDULLARY BONE CORRELATED TO DIFFERENT STAGES OF THE EGG FORMATION IN HENS Scientific Papers Animal Science and Biotechnologies laying fowl medullary bone osteoclast osteoblast |
| title | HISTOLOGICAL ASPECTS OF THE MEDULLARY BONE CORRELATED TO DIFFERENT STAGES OF THE EGG FORMATION IN HENS |
| title_full | HISTOLOGICAL ASPECTS OF THE MEDULLARY BONE CORRELATED TO DIFFERENT STAGES OF THE EGG FORMATION IN HENS |
| title_fullStr | HISTOLOGICAL ASPECTS OF THE MEDULLARY BONE CORRELATED TO DIFFERENT STAGES OF THE EGG FORMATION IN HENS |
| title_full_unstemmed | HISTOLOGICAL ASPECTS OF THE MEDULLARY BONE CORRELATED TO DIFFERENT STAGES OF THE EGG FORMATION IN HENS |
| title_short | HISTOLOGICAL ASPECTS OF THE MEDULLARY BONE CORRELATED TO DIFFERENT STAGES OF THE EGG FORMATION IN HENS |
| title_sort | histological aspects of the medullary bone correlated to different stages of the egg formation in hens |
| topic | laying fowl medullary bone osteoclast osteoblast |
| url | https://spasb.ro/index.php/public_html/article/view/1789 |
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