Epimerization of Deoxynivalenol by the <i>Devosia</i> Strain A6-243 Assisted by Pyrroloquinoline Quinone
Deoxynivalenol (DON) is a secondary metabolite produced by several <i>Fusarium</i> species that is hazardous to humans and animals after entering food chains. In this study, by adding cofactors, the <i>Devosia</i> strain A6-243 is identified as the DON-transforming bacteria f...
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MDPI AG
2021-12-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/14/1/16 |
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| author | Hui Gao Jiafeng Niu Hua Yang Zhaoxin Lu Libang Zhou Fanqiang Meng Fengxia Lu Meirong Chen |
| author_facet | Hui Gao Jiafeng Niu Hua Yang Zhaoxin Lu Libang Zhou Fanqiang Meng Fengxia Lu Meirong Chen |
| author_sort | Hui Gao |
| collection | DOAJ |
| description | Deoxynivalenol (DON) is a secondary metabolite produced by several <i>Fusarium</i> species that is hazardous to humans and animals after entering food chains. In this study, by adding cofactors, the <i>Devosia</i> strain A6-243 is identified as the DON-transforming bacteria from a bacterial consortium with the ability to biotransform DON of <i>Pseudomonas</i> sp. B6-24 and <i>Devosia</i> strain A6-243, and its effect on the biotransformation process of DON is studied. The <i>Devosia</i> strain A6-243 completely biotransformed 100 μg/mL of DON with the assistance of the exogenous addition of PQQ (pyrroloquinoline quinone) within 48 h and produced non-toxic 3-epi-DON (3-epi-deoxynivalenol), while <i>Pseudomonas</i> sp. B6-24 was not able to biotransform DON, but it had the ability to generate PQQ. Moreover, the <i>Devosia</i> strain A6-243 not only degraded DON, but also exhibited the ability to degrade 3-keto-DON (3-keto-deoxynivalenol) with the same product 3-epi-DON, indicating that DON epimerization by the <i>Devosia</i> strain A6-243 is a two-step enzymatic reaction. The most suitable conditions for the biodegradation process of the <i>Devosia</i> strain A6-243 were a temperature of 16–37 °C and pH 7.0–10, with 15–30 μM PQQ. In addition, the <i>Devosia</i> strain A6-243 was found to completely remove DON (6.7 μg/g) from DON-contaminated wheat. The results presented a reference for screening microorganisms with the ability of biotransform DON and laid a foundation for the development of enzymes for the detoxification of mycotoxins in grain and its products. |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-10T00:24:37Z |
| publishDate | 2021-12-01 |
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| spelling | doaj.art-45fd3248fec7465e8ff8c29dad0c94f72023-11-23T15:35:39ZengMDPI AGToxins2072-66512021-12-011411610.3390/toxins14010016Epimerization of Deoxynivalenol by the <i>Devosia</i> Strain A6-243 Assisted by Pyrroloquinoline QuinoneHui Gao0Jiafeng Niu1Hua Yang2Zhaoxin Lu3Libang Zhou4Fanqiang Meng5Fengxia Lu6Meirong Chen7Laboratory of Enzyme Engineering, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaLaboratory of Enzyme Engineering, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaLaboratory of Enzyme Engineering, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaLaboratory of Enzyme Engineering, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaLaboratory of Enzyme Engineering, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaLaboratory of Enzyme Engineering, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaLaboratory of Enzyme Engineering, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaLaboratory of Enzyme Engineering, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaDeoxynivalenol (DON) is a secondary metabolite produced by several <i>Fusarium</i> species that is hazardous to humans and animals after entering food chains. In this study, by adding cofactors, the <i>Devosia</i> strain A6-243 is identified as the DON-transforming bacteria from a bacterial consortium with the ability to biotransform DON of <i>Pseudomonas</i> sp. B6-24 and <i>Devosia</i> strain A6-243, and its effect on the biotransformation process of DON is studied. The <i>Devosia</i> strain A6-243 completely biotransformed 100 μg/mL of DON with the assistance of the exogenous addition of PQQ (pyrroloquinoline quinone) within 48 h and produced non-toxic 3-epi-DON (3-epi-deoxynivalenol), while <i>Pseudomonas</i> sp. B6-24 was not able to biotransform DON, but it had the ability to generate PQQ. Moreover, the <i>Devosia</i> strain A6-243 not only degraded DON, but also exhibited the ability to degrade 3-keto-DON (3-keto-deoxynivalenol) with the same product 3-epi-DON, indicating that DON epimerization by the <i>Devosia</i> strain A6-243 is a two-step enzymatic reaction. The most suitable conditions for the biodegradation process of the <i>Devosia</i> strain A6-243 were a temperature of 16–37 °C and pH 7.0–10, with 15–30 μM PQQ. In addition, the <i>Devosia</i> strain A6-243 was found to completely remove DON (6.7 μg/g) from DON-contaminated wheat. The results presented a reference for screening microorganisms with the ability of biotransform DON and laid a foundation for the development of enzymes for the detoxification of mycotoxins in grain and its products.https://www.mdpi.com/2072-6651/14/1/16deoxynivalenolepimerizationpyrroloquinoline quinone<i>Devosia</i><i>Pseudomonas</i> |
| spellingShingle | Hui Gao Jiafeng Niu Hua Yang Zhaoxin Lu Libang Zhou Fanqiang Meng Fengxia Lu Meirong Chen Epimerization of Deoxynivalenol by the <i>Devosia</i> Strain A6-243 Assisted by Pyrroloquinoline Quinone Toxins deoxynivalenol epimerization pyrroloquinoline quinone <i>Devosia</i> <i>Pseudomonas</i> |
| title | Epimerization of Deoxynivalenol by the <i>Devosia</i> Strain A6-243 Assisted by Pyrroloquinoline Quinone |
| title_full | Epimerization of Deoxynivalenol by the <i>Devosia</i> Strain A6-243 Assisted by Pyrroloquinoline Quinone |
| title_fullStr | Epimerization of Deoxynivalenol by the <i>Devosia</i> Strain A6-243 Assisted by Pyrroloquinoline Quinone |
| title_full_unstemmed | Epimerization of Deoxynivalenol by the <i>Devosia</i> Strain A6-243 Assisted by Pyrroloquinoline Quinone |
| title_short | Epimerization of Deoxynivalenol by the <i>Devosia</i> Strain A6-243 Assisted by Pyrroloquinoline Quinone |
| title_sort | epimerization of deoxynivalenol by the i devosia i strain a6 243 assisted by pyrroloquinoline quinone |
| topic | deoxynivalenol epimerization pyrroloquinoline quinone <i>Devosia</i> <i>Pseudomonas</i> |
| url | https://www.mdpi.com/2072-6651/14/1/16 |
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