Differential expression of the heat shock protein Hsp70 in natural populations of the tilapia, <it>Sarotherodon melanotheron</it>, acclimatised to a range of environmental salinities
<p>Abstract</p> <p>Background</p> <p>The relationship between environmental variation and induction of heat shock proteins <it>(Hsps) </it>has been much documented under experimental conditions. However, very little is known about such induction in natural p...
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| Format: | Article |
| Language: | English |
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BMC
2010-04-01
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| Series: | BMC Ecology |
| Online Access: | http://www.biomedcentral.com/1472-6785/10/11 |
| _version_ | 1818978888047394816 |
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| author | McKenzie David J Bonhomme François Tine Mbaye Durand Jean-Dominique |
| author_facet | McKenzie David J Bonhomme François Tine Mbaye Durand Jean-Dominique |
| author_sort | McKenzie David J |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The relationship between environmental variation and induction of heat shock proteins <it>(Hsps) </it>has been much documented under experimental conditions. However, very little is known about such induction in natural populations acclimatised to prevailing environmental conditions. Furthermore, while induction of stress proteins has been well documented in response to environmental contaminants and thermal stressors, little is known about whether factors, such as extreme salinity, are also potential inductors. The black-chinned tilapia <it>Sarotherodon melanotheron </it>is unusual for its ability to colonise estuarine environments in West Africa that are characterised by extremely high salinities. The relationships between mRNA levels of the 70 kDa heat shock protein (<it>Hsp70</it>) and Na<sup>+</sup>, K<sup>+</sup>-ATPase1α (<it>Naka</it>) in the gills, environmental salinity, and a life-history trait (condition factor) were investigated in wild populations of this species sampled from three locations in the Saloum estuary, at salinities ranging from 40 to 100 psu.</p> <p>Results</p> <p>The highest <it>Hsp70 </it>and <it>Naka </it>mRNA levels, and the poorest condition factors were recorded in the most saline sampling site (100 psu). The <it>Hsp70 </it>and <it>Naka </it>mRNA were correlated amongst themselves and showed a direct positive correlation with environmental salinity, and a negative correlation with fish condition factor. Thus, the <it>Hsp70 </it>is constitutively overexpressed by <it>S. melanotheron </it>acclimatised to extreme hypersalinity.</p> <p>Conclusions</p> <p>These results indicate that, although <it>S. melanotheron </it>can colonise extremely saline environments, the overexpression of <it>Hsp70 </it>combined with the higher <it>Naka </it>mRNA expression reveals that this represents a chronic stress. The induction of <it>Hsp70 </it>was, therefore, a biomarker of chronic hyper-osmotic stress which presumably can be linked to the impaired growth performance and precocious reproduction that have been demonstrated in the populations at the extremely saline sites.</p> |
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| institution | Directory Open Access Journal |
| issn | 1472-6785 |
| language | English |
| last_indexed | 2024-12-20T16:50:47Z |
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| spelling | doaj.art-45fd3f0aea474d65a655274dcde41ea22022-12-21T19:32:49ZengBMCBMC Ecology1472-67852010-04-011011110.1186/1472-6785-10-11Differential expression of the heat shock protein Hsp70 in natural populations of the tilapia, <it>Sarotherodon melanotheron</it>, acclimatised to a range of environmental salinitiesMcKenzie David JBonhomme FrançoisTine MbayeDurand Jean-Dominique<p>Abstract</p> <p>Background</p> <p>The relationship between environmental variation and induction of heat shock proteins <it>(Hsps) </it>has been much documented under experimental conditions. However, very little is known about such induction in natural populations acclimatised to prevailing environmental conditions. Furthermore, while induction of stress proteins has been well documented in response to environmental contaminants and thermal stressors, little is known about whether factors, such as extreme salinity, are also potential inductors. The black-chinned tilapia <it>Sarotherodon melanotheron </it>is unusual for its ability to colonise estuarine environments in West Africa that are characterised by extremely high salinities. The relationships between mRNA levels of the 70 kDa heat shock protein (<it>Hsp70</it>) and Na<sup>+</sup>, K<sup>+</sup>-ATPase1α (<it>Naka</it>) in the gills, environmental salinity, and a life-history trait (condition factor) were investigated in wild populations of this species sampled from three locations in the Saloum estuary, at salinities ranging from 40 to 100 psu.</p> <p>Results</p> <p>The highest <it>Hsp70 </it>and <it>Naka </it>mRNA levels, and the poorest condition factors were recorded in the most saline sampling site (100 psu). The <it>Hsp70 </it>and <it>Naka </it>mRNA were correlated amongst themselves and showed a direct positive correlation with environmental salinity, and a negative correlation with fish condition factor. Thus, the <it>Hsp70 </it>is constitutively overexpressed by <it>S. melanotheron </it>acclimatised to extreme hypersalinity.</p> <p>Conclusions</p> <p>These results indicate that, although <it>S. melanotheron </it>can colonise extremely saline environments, the overexpression of <it>Hsp70 </it>combined with the higher <it>Naka </it>mRNA expression reveals that this represents a chronic stress. The induction of <it>Hsp70 </it>was, therefore, a biomarker of chronic hyper-osmotic stress which presumably can be linked to the impaired growth performance and precocious reproduction that have been demonstrated in the populations at the extremely saline sites.</p>http://www.biomedcentral.com/1472-6785/10/11 |
| spellingShingle | McKenzie David J Bonhomme François Tine Mbaye Durand Jean-Dominique Differential expression of the heat shock protein Hsp70 in natural populations of the tilapia, <it>Sarotherodon melanotheron</it>, acclimatised to a range of environmental salinities BMC Ecology |
| title | Differential expression of the heat shock protein Hsp70 in natural populations of the tilapia, <it>Sarotherodon melanotheron</it>, acclimatised to a range of environmental salinities |
| title_full | Differential expression of the heat shock protein Hsp70 in natural populations of the tilapia, <it>Sarotherodon melanotheron</it>, acclimatised to a range of environmental salinities |
| title_fullStr | Differential expression of the heat shock protein Hsp70 in natural populations of the tilapia, <it>Sarotherodon melanotheron</it>, acclimatised to a range of environmental salinities |
| title_full_unstemmed | Differential expression of the heat shock protein Hsp70 in natural populations of the tilapia, <it>Sarotherodon melanotheron</it>, acclimatised to a range of environmental salinities |
| title_short | Differential expression of the heat shock protein Hsp70 in natural populations of the tilapia, <it>Sarotherodon melanotheron</it>, acclimatised to a range of environmental salinities |
| title_sort | differential expression of the heat shock protein hsp70 in natural populations of the tilapia it sarotherodon melanotheron it acclimatised to a range of environmental salinities |
| url | http://www.biomedcentral.com/1472-6785/10/11 |
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